important study from PhD indicating which drugs do what exactly when fighting Lyme..... results you need to know about

[Guest guest]

important study from PhD indicating which drugs do what exactly when fighting

Lyme.......surprising results you need to know about

                   blessings

                           Shan

Evaluation of in-vitro antibiotic susceptibility of different morphological

forms of Borrelia burgdorferi.http://www.ncbi.nlm.nih.gov/pubmed/21753890

    

Sapi E, Kaur N, Anyanwu S, Luecke DF, Datar A, Patel S, Rossi M, Stricker RB.

SourceLyme Disease Research Group, Department of Biology and Environmental

Sciences, University of New Haven, New Haven, CT, USA;

Abstract

BACKGROUND: Lyme disease is a tick-borne illness caused by the spirochete

Borrelia burgdorferi. Although antibiotic therapy is usually effective early in

the disease, relapse may occur when administration of antibiotics is

discontinued. Studies have suggested that resistance and recurrence of Lyme

disease might be due to formation of different morphological forms of B.

burgdorferi, namely round bodies (cysts) and biofilm-like colonies. Better

understanding of the effect of antibiotics on all morphological forms of B.

burgdorferi is therefore crucial to provide effective therapy for Lyme disease.

METHODS: Three morphological forms of B. burgdorferi (spirochetes, round bodies,

and biofilm-like colonies) were generated using novel culture methods. Minimum

inhibitory concentration and minimum bactericidal concentration of five

antimicrobial agents (doxycycline, amoxicillin, tigecycline, metronidazole, and

tinidazole) against spirochetal forms of B. burgdorferi were evaluated using the

standard published microdilution technique. The susceptibility of spirochetal

and round body forms to the antibiotics was then tested using fluorescent

microscopy (BacLightâ„¢ viability staining) and dark field microscopy (direct

cell counting), and these results were compared with the microdilution

technique. Qualitative and quantitative effects of the antibiotics against

biofilm-like colonies were assessed using fluorescent microscopy and dark field

microscopy, respectively.

RESULTS: Doxycycline reduced spirochetal structures ∼90% but increased the

number of round body forms about twofold. Amoxicillin reduced spirochetal forms

by ∼85%-90% and round body forms by ∼68%, while treatment with metronidazole

led to reduction of spirochetal structures by ∼90% and round body forms by

∼80%. Tigecycline and tinidazole treatment reduced both spirochetal and round

body forms by ∼80%-90%. When quantitative effects on biofilm-like colonies

were evaluated, the five antibiotics reduced formation of these colonies by only

30%-55%. In terms of qualitative effects, only tinidazole reduced viable

organisms by ∼90%. Following treatment with the other antibiotics, viable

organisms were detected in 70%-85% of the biofilm-like colonies.

CONCLUSION: Antibiotics have varying effects on the different morphological

forms of B. burgdorferi. Persistence of viable organisms in round body forms and

biofilm-like colonies may explain treatment failure and persistent symptoms

following antibiotic therapy of Lyme disease.

PMID:21753890 [PubMed]

PMCID:PMC3132871

Free PMC Article

Images from this publication.........

[Guest guest]

the Bob Beck Lyme protocol, uses electric current and strong pulsed

electromagnetism (to create pulses of electric current deep in tissue) to drive

lyme out of the body.

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On 28-Apr-2012, at 1:13 AM, surpriseshan2@... wrote:

> important study from PhD indicating which drugs do what exactly when fighting

Lyme.......surprising results you need to know about

> blessings

> Shan

>

> Evaluation of in-vitro antibiotic susceptibility of different morphological

forms of Borrelia burgdorferi.http://www.ncbi.nlm.nih.gov/pubmed/21753890

>

>

>

> Sapi E, Kaur N, Anyanwu S, Luecke DF, Datar A, Patel S, Rossi M, Stricker RB.

> SourceLyme Disease Research Group, Department of Biology and Environmental

Sciences, University of New Haven, New Haven, CT, USA;

>

> Abstract

>

> BACKGROUND: Lyme disease is a tick-borne illness caused by the spirochete

Borrelia burgdorferi. Although antibiotic therapy is usually effective early in

the disease, relapse may occur when administration of antibiotics is

discontinued. Studies have suggested that resistance and recurrence of Lyme

disease might be due to formation of different morphological forms of B.

burgdorferi, namely round bodies (cysts) and biofilm-like colonies. Better

understanding of the effect of antibiotics on all morphological forms of B.

burgdorferi is therefore crucial to provide effective therapy for Lyme disease.

>

> METHODS: Three morphological forms of B. burgdorferi (spirochetes, round

bodies, and biofilm-like colonies) were generated using novel culture methods.

Minimum inhibitory concentration and minimum bactericidal concentration of five

antimicrobial agents (doxycycline, amoxicillin, tigecycline, metronidazole, and

tinidazole) against spirochetal forms of B. burgdorferi were evaluated using the

standard published microdilution technique. The susceptibility of spirochetal

and round body forms to the antibiotics was then tested using fluorescent

microscopy (BacLightâ„¢ viability staining) and dark field microscopy (direct

cell counting), and these results were compared with the microdilution

technique. Qualitative and quantitative effects of the antibiotics against

biofilm-like colonies were assessed using fluorescent microscopy and dark field

microscopy, respectively.

>

> RESULTS: Doxycycline reduced spirochetal structures ∼90% but increased the

number of round body forms about twofold. Amoxicillin reduced spirochetal forms

by ∼85%-90% and round body forms by ∼68%, while treatment with metronidazole

led to reduction of spirochetal structures by ∼90% and round body forms by

∼80%. Tigecycline and tinidazole treatment reduced both spirochetal and round

body forms by ∼80%-90%. When quantitative effects on biofilm-like colonies

were evaluated, the five antibiotics reduced formation of these colonies by only

30%-55%. In terms of qualitative effects, only tinidazole reduced viable

organisms by ∼90%. Following treatment with the other antibiotics, viable

organisms were detected in 70%-85% of the biofilm-like colonies.

>

> CONCLUSION: Antibiotics have varying effects on the different morphological

forms of B. burgdorferi. Persistence of viable organisms in round body forms and

biofilm-like colonies may explain treatment failure and persistent symptoms

following antibiotic therapy of Lyme disease.

>

> PMID:21753890 [PubMed]

> PMCID:PMC3132871

> Free PMC Article

>

> Images from this publication.........

>

>