Guest guest Posted October 22, 2009 Report Share Posted October 22, 2009 This will give you more than you thought you needed to know, lol. It's from Dr. Woeller's AutismActionPlan.org site which I am a member of. Biofilm Protocol Biofllms are a polysaccharide-type matrix (think of a large gelatinous glob) secreted not only by pathogenic and opportunistic bacteria and yeast, but the normal bacteria (flora) in our digestive system as well. The opportunistic pathogens secrete these biofilms as a defense mechanism to prevent immune detection which allows them to survive in an otherwise hostile environment. Unfortunately, with expansion of abnormal bacteria and yeast biofilms, the normal flora in the digestive system are reduced in number and overtime the digestive system becomes more and more stressed with increasing numbers of bad bacteria and yeast. In many situations these bacteria and yeast secrete toxins which adversely affect health. An example of biofilms is dental cavities. Bacteria in the mouth can produce biofilms which lead to cavity formation. A brand of mouthwash is now available called Biotene (found in most drug stores) which is specifically designed to help breakdown biofilms in the mouth. The biofilms are held together by a combination of negatively charged minerals (aka. ions) such as calcium, magnesium and iron. In fact, iron seems to play a role in how many different types of pathogenic bacteria evade the immune system. Because these biofilms are negatively charged with ionic minerals they are vulnerable to a certain chelator called EDTA (ethylenediaminetetraacetic acid). The EDTA helps bind these ions which makes the biofilm less stable. The addition of specific enzymes helps to breakdown the polysaccharide matrix, and the combination of the two – enzyme plus EDTA – makes the bacteria and yeast encased in the biofilm sludge exposed and vulnerable to attack. This not only allows the immune system access to the hiding pathogens, but antibiotics, anti-fungals or natural remedies such as herbs are now able to adversely affect the colonies of hiding bacteria and yeast. The Biofilm Protocol is a therapy that can be employed in those individuals where conventional anti-bacterial or anti-yeast treatments have not resolved the issue. Listed here are a few things to consider with the use of this protocol: • Lack of stool culture findings for yeast and/or bacteria – but, individual seems to improve when placed on antibiotics or antifungal medication. • Adverse behavior in autism-spectrum disorders such as aggression, agitation, head-banging and others that is thought to be gut related, but no direct evidence of digestive pathogens. • Individuals with Obsessive-Compulsive Disorder (OCD), perseverative and/or repetitive behaviors – who tend to get worse in the spring and/or fall, yet do not test positive for strep infections. • Persistent and unresolved yeast and/or bacteria as evidenced on Organic Acid Test from Great Plains Laboratory (such as arabinose for yeast or HPHPA for clostridia bacteria). Listed below is an outline for the traditional Biofilm Protocol. NOTE: See Suggested Supplement List at end of this page. Step #1 - Lysis (breakdown) and Detachment of the Polysaccharide Matrix, i.e. Biofilm Take remedy on an empty stomach (30 to 45 minutes before food) minimally 30 to 60 minutes before Step #2 • Interfase Plus (Klaire Labs or New Beginnings) – 2 to 4 capsules before or in-between meals (30 minutes prior or 90 minutes after meals). This product is available from Klaire Labs (http://www.klaire.com/K-INTP_proddetail.htm) or 888-488-2488. You must use an access code. New Beginnings also requires an access code. NOTE: This combination product includes specific digestive enzymes that help degrade the biofilm matrix, as well as disodium EDTA to help bind the biofilm matrix ions such as iron. EDTA is poorly absorbed orally so there is no issue of systemic metal detoxification. Step #2 – Attacking the Microbe(s) – yeast and bacteria • Vancomycin (prescription antibiotic) dosed at 15mg/kg per dose. Example: 40lbs (40 divided by 2.2 = 18) child weighs 18 kilograms (kg). 18kg x 15mg = 272mg. Vancomycin commonly come in 125, 250, and 500mg capsules. Rounding down the 250mg capsule would be appropriate for this child. Vancomycin can be compounded into an oral suspension as well from specialized pharmacies. • Strep Bacteria – if you are suspicious that strep bacteria are causing continued problems, such as that seen in OCD, TIC's, perseverative behavior than using a penicillin type medication such as amoxicillin may be warranted. • Other antibiotic (prescription) examples that could be used: Alinia, Flagyl, Keflex, Zithromax. • Natural Remedies (herbs, supplements): o Grapefruit Seed Extract (GSE) o Oil of Oregano (OO) o Caprylic Acid (example: MCT Oil from New Beginnings) o Uva Ursi (UU) o Combination Herbal Remedies for yeast and bacteria (example: Candida Defense Formula – from New Beginnings) NOTE: There is no exact anti-microbe format to follow. If you do not have access to an antibiotic (or do not feel comfortable using one) than you can employ more herbal remedies such as those listed above. With the natural remedies it is possible to rotate between different ones to help maximize the effect. One example is to rotate between GSE, OO, MCT, UU or others every 3 to 5 days. Step #3 – Cleaning Up This is a critical step in this whole biofilm treatment program. Absolutely DO NOT SKIP. Give this remedy(s) 1-2 hours after Step #2 - if possible or at night (depending on when you give the biofilm protocol remedies). Step #3 is all about binding the broken-down matrix and other byproducts of yeast and bacteria die-off. Also, because biofilms are held together by ions such as iron, calcium, magnesium, they may encase heavy metals such as mercury, aluminum, lead, etc. The EDTA that was used in Step #1 will help bind some of these toxins, but also the incorporation of other products listed below can help as well. Remedy Examples: All can be mixed in juice (or food) if needed. • Activated Charcoal (approximately 400 to 500mg) – This is best mixed in juice - and/or • PectaSol-C (Modified Citrus Pectin) – available from Research Nutritionals at www.researchednutritionals.com – 1 to 2 scoops. Also requires an access code. NOTE: Pecta-Sol is also a source of fiber beneficial for digestive support, and does help diminish the level of heavy metal toxins in the body gently overtime as well. • Apple Pectin – can find at most health food stores – 1 to 2 tablespoons. Apple Pectin is another source of gentle fiber - and/or * TruFiber - this is a fiber support product from New Beginnings - generally 1/2 to 1 scoop twice daily NOTE: In some cases the use of many different products at the same time can be used. One of the effects of these agents is to bind toxins generated in the gut and prevent them from being absorbed back into to body. Once these gut toxins are bound to one of these remedies that are effectively eliminated through the stool. Other Considerations: As part of an ongoing biomedical treatment program the continuation of dietary modifications such as healthy and non-toxic foods (such as organic and hormone-free), as well as low sugar, gluten and casein-free, etc. is critical. The addition of other food items can be helpful as well to help promote normal flora and health to the digestive system: • Cultured Vegetables • Kefir – coconut milk • Kombucha - Synergy Drinks (they have a wide variety) Also, the use of probiotics, essential fatty acids, and other basic vitamins (multi-vitamin) and minerals is important. If you are using an antibiotic such as Vancomycin, Amoxicillin, Keflex, etc. than the additional use of Nystatin (minimally) is recommended to prevent too much yeast overgrowth. Also, the use of natural anti-yeast herbs such as capyrilic acid (i.e. MCT Oil), grapefruit seed extract, oil of oregano, garlic extracts, etc. are helpful too. Final Comment: The Biofilm Protocol is not necessarily done in isolation – meaning you would not want to discontinue or avoid other therapies such a dietary intervention (GF/CF, Specific Carbohydrate, Low Oxalate), Methyl-B12 therapy, etc. when doing this program. However, it is important to remember that the Biofilm Protocol is meant to eradicate suspected persistent infections that are not being resolved through normal measures. The process takes time – sometimes 2 to 6 months (longer in some cases) – and should not be viewed as a short-term fix. Also, each person is unique in how they will respond to this program. Some kids who have deep-seated infections may experience irritability, agitation or exacerbation of other behavior in the initial stages of this program. These typical die-off reactions are not uncommon. This is why Step #3 is so important to help bind the intestinal toxins that are produced from pathogenic bacteria and yeast die-off. You need to move at your own pace with this program, expecting to do some experimentation with different remedies to find the right combination for your child. The ultimate goal is to restore normal flora and normal flora biofilm in the digestive system. This takes time, and the process will not happen overnight. However, patience and persistence will pay-off overtime. It is generally recommended to do the Biofilm Protocol steps one time per day in the beginning. I would advocate doing this for at 3 to 6 weeks before adding a second dose. Again, it is based on an individuals tolerance. Suggested Supplement List: Klaire Labs (http://www.klaire.com/K-INTP_proddetail.htm) or 888-488-2488. • Interfase Plus * TruFiber - no access code required New Beginnings (through supplement section): • Grapefruits Seed Extract – 3 to 5 drops • Oregano Oil (Oreganol) - 1 to 2 capsules • MCT Oil – 1 to 2 tablespoons • Uva Ursi – 1 to 2 capsules Researched Nutritionals (www.researchednutritionals.com): • Pecta-Sol C – 1 to 2 scoops Health Food Store: • Activated Charcoal – approximately 250 to 500mg • Apple Pectin – 1 to 2 tablespoons Final Comment – whenever you are starting a new therapy – particularly one that involves the treatment of intestinal bacteria and yeast – it is important to start low and go slow. What this means is be patient with your approach as you implement more remedies. You can also increase the dosage of a supplement or herbal remedy overtime as the treatment process moves forward. It is highly recommended that you have some testing done for your child - minimally an Organic Acid Test and Comprehensive Digestive Stool Analysis (see Lab Ordering Section) - prior to attempting this protocol. The reason is you want to try and identify particular gut pathogens so you have a better idea of what to use in the protocol. Also, if you suspect that your child is constipated or has a sluggish bowel than it is advisable to have this evaluated before implementing this program. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted October 24, 2009 Report Share Posted October 24, 2009 I am hoping along with our other biomedical treatments, the combination will do just that. > > > From: G <luckylot@...> > Subject: [ ] Re: biofilm protocol > > Date: Thursday, October 22, 2009, 9:57 AM > > >  > > > > This will give you more than you thought you needed to know, lol. It's from Dr. Woeller's AutismActionPlan. org site which I am a member of. > > Biofilm Protocol > > Biofllms are a polysaccharide- type matrix (think of a large gelatinous glob) secreted not only by pathogenic and opportunistic bacteria and yeast, but the normal bacteria (flora) in our digestive system as well. The opportunistic pathogens secrete these biofilms as a defense mechanism to prevent immune detection which allows them to survive in an otherwise hostile environment. Unfortunately, with expansion of abnormal bacteria and yeast biofilms, the normal flora in the digestive system are reduced in number and overtime the digestive system becomes more and more stressed with increasing numbers of bad bacteria and yeast. In many situations these bacteria and yeast secrete toxins which adversely affect health. An example of biofilms is dental cavities. Bacteria in the mouth can produce biofilms which lead to cavity formation. A brand of mouthwash is now available called Biotene (found in most drug stores) which is specifically designed to help breakdown > biofilms in the mouth. > > The biofilms are held together by a combination of negatively charged minerals (aka. ions) such as calcium, magnesium and iron. In fact, iron seems to play a role in how many different types of pathogenic bacteria evade the immune system. Because these biofilms are negatively charged with ionic minerals they are vulnerable to a certain chelator called EDTA (ethylenediaminetet raacetic acid). The EDTA helps bind these ions which makes the biofilm less stable. The addition of specific enzymes helps to breakdown the polysaccharide matrix, and the combination of the two †" enzyme plus EDTA †" makes the bacteria and yeast encased in the biofilm sludge exposed and vulnerable to attack. This not only allows the immune system access to the hiding pathogens, but antibiotics, anti-fungals or natural remedies such as herbs are now able to adversely affect the colonies of hiding bacteria and yeast. > > The Biofilm Protocol is a therapy that can be employed in those individuals where conventional anti-bacterial or anti-yeast treatments have not resolved the issue. Listed here are a few things to consider with the use of this protocol: > > • Lack of stool culture findings for yeast and/or bacteria †" but, individual seems to improve when placed on antibiotics or antifungal medication. > • Adverse behavior in autism-spectrum disorders such as aggression, agitation, head-banging and others that is thought to be gut related, but no direct evidence of digestive pathogens. > • Individuals with Obsessive-Compulsiv e Disorder (OCD), perseverative and/or repetitive behaviors †" who tend to get worse in the spring and/or fall, yet do not test positive for strep infections. > • Persistent and unresolved yeast and/or bacteria as evidenced on Organic Acid Test from Great Plains Laboratory (such as arabinose for yeast or HPHPA for clostridia bacteria). > > Listed below is an outline for the traditional Biofilm Protocol. > > NOTE: See Suggested Supplement List at end of this page. > > Step #1 - Lysis (breakdown) and Detachment of the Polysaccharide Matrix, i.e. Biofilm > > Take remedy on an empty stomach (30 to 45 minutes before food) minimally 30 to 60 minutes before Step #2 > > • Interfase Plus (Klaire Labs or New Beginnings) †" 2 to 4 capsules before or in-between meals (30 minutes prior or 90 minutes after meals). This product is available from Klaire Labs (http://www.klaire. com/K-INTP_ proddetail. htm) or 888-488-2488. You must use an access code. New Beginnings also requires an access code. > > NOTE: This combination product includes specific digestive enzymes that help degrade the biofilm matrix, as well as disodium EDTA to help bind the biofilm matrix ions such as iron. EDTA is poorly absorbed orally so there is no issue of systemic metal detoxification. > > Step #2 †" Attacking the Microbe(s) †" yeast and bacteria > > • Vancomycin (prescription antibiotic) dosed at 15mg/kg per dose. > > Example: 40lbs (40 divided by 2.2 = 18) child weighs 18 kilograms (kg). 18kg x 15mg = 272mg. Vancomycin commonly come in 125, 250, and 500mg capsules. Rounding down the 250mg capsule would be appropriate for this child. Vancomycin can be compounded into an oral suspension as well from specialized pharmacies. > > • Strep Bacteria †" if you are suspicious that strep bacteria are causing continued problems, such as that seen in OCD, TIC's, perseverative behavior than using a penicillin type medication such as amoxicillin may be warranted. > > • Other antibiotic (prescription) examples that could be used: Alinia, Flagyl, Keflex, Zithromax. > > • Natural Remedies (herbs, supplements) : > > o Grapefruit Seed Extract (GSE) > o Oil of Oregano (OO) > o Caprylic Acid (example: MCT Oil from New Beginnings) > o Uva Ursi (UU) > > o Combination Herbal Remedies for yeast and bacteria (example: Candida Defense Formula †" from New Beginnings) > > NOTE: There is no exact anti-microbe format to follow. If you do not have access to an antibiotic (or do not feel comfortable using one) than you can employ more herbal remedies such as those listed above. With the natural remedies it is possible to rotate between different ones to help maximize the effect. One example is to rotate between GSE, OO, MCT, UU or others every 3 to 5 days. > > Step #3 †" Cleaning Up > > This is a critical step in this whole biofilm treatment program. Absolutely DO NOT SKIP. > > Give this remedy(s) 1-2 hours after Step #2 - if possible or at night (depending on when you give the biofilm protocol remedies). Step #3 is all about binding the broken-down matrix and other byproducts of yeast and bacteria die-off. Also, because biofilms are held together by ions such as iron, calcium, magnesium, they may encase heavy metals such as mercury, aluminum, lead, etc. The EDTA that was used in Step #1 will help bind some of these toxins, but also the incorporation of other products listed below can help as well. > > Remedy Examples: > > All can be mixed in juice (or food) if needed. > > • Activated Charcoal (approximately 400 to 500mg) †" This is best mixed in juice - and/or > • PectaSol-C (Modified Citrus Pectin) †" available from Research Nutritionals at www.researchednutri tionals.com †" 1 to 2 scoops. Also requires an access code. > > NOTE: Pecta-Sol is also a source of fiber beneficial for digestive support, and does help diminish the level of heavy metal toxins in the body gently overtime as well. > > • Apple Pectin †" can find at most health food stores †" 1 to 2 tablespoons. Apple Pectin is another source of gentle fiber - and/or > > * TruFiber - this is a fiber support product from New Beginnings - generally 1/2 to 1 scoop twice daily > > NOTE: In some cases the use of many different products at the same time can be used. One of the effects of these agents is to bind toxins generated in the gut and prevent them from being absorbed back into to body. Once these gut toxins are bound to one of these remedies that are effectively eliminated through the stool. > > Other Considerations: > > As part of an ongoing biomedical treatment program the continuation of dietary modifications such as healthy and non-toxic foods (such as organic and hormone-free) , as well as low sugar, gluten and casein-free, etc. is critical. The addition of other food items can be helpful as well to help promote normal flora and health to the digestive system: > > • Cultured Vegetables > • Kefir †" coconut milk > • Kombucha - Synergy Drinks (they have a wide variety) > > Also, the use of probiotics, essential fatty acids, and other basic vitamins (multi-vitamin) and minerals is important. > > If you are using an antibiotic such as Vancomycin, Amoxicillin, Keflex, etc. than the additional use of Nystatin (minimally) is recommended to prevent too much yeast overgrowth. Also, the use of natural anti-yeast herbs such as capyrilic acid (i.e. MCT Oil), grapefruit seed extract, oil of oregano, garlic extracts, etc. are helpful too. > > Final Comment: > > The Biofilm Protocol is not necessarily done in isolation †" meaning you would not want to discontinue or avoid other therapies such a dietary intervention (GF/CF, Specific Carbohydrate, Low Oxalate), Methyl-B12 therapy, etc. when doing this program. However, it is important to remember that the Biofilm Protocol is meant to eradicate suspected persistent infections that are not being resolved through normal measures. > > The process takes time †" sometimes 2 to 6 months (longer in some cases) †" and should not be viewed as a short-term fix. > > Also, each person is unique in how they will respond to this program. Some kids who have deep-seated infections may experience irritability, agitation or exacerbation of other behavior in the initial stages of this program. These typical die-off reactions are not uncommon. This is why Step #3 is so important to help bind the intestinal toxins that are produced from pathogenic bacteria and yeast die-off. > > You need to move at your own pace with this program, expecting to do some experimentation with different remedies to find the right combination for your child. The ultimate goal is to restore normal flora and normal flora biofilm in the digestive system. This takes time, and the process will not happen overnight. However, patience and persistence will pay-off overtime. It is generally recommended to do the Biofilm Protocol steps one time per day in the beginning. I would advocate doing this for at 3 to 6 weeks before adding a second dose. Again, it is based on an individuals tolerance. > > Suggested Supplement List: > Klaire Labs (http://www.klaire. com/K-INTP_ proddetail. htm) or 888-488-2488. > > • Interfase Plus > > * TruFiber - no access code required > > New Beginnings (through supplement section): > > • Grapefruits Seed Extract †" 3 to 5 drops > • Oregano Oil (Oreganol) - 1 to 2 capsules > • MCT Oil †" 1 to 2 tablespoons > • Uva Ursi †" 1 to 2 capsules > > Researched Nutritionals (www.researchednutr itionals. com): > > • Pecta-Sol C †" 1 to 2 scoops > > Health Food Store: > > • Activated Charcoal †" approximately 250 to 500mg > • Apple Pectin †" 1 to 2 tablespoons > > Final Comment †" whenever you are starting a new therapy †" particularly one that involves the treatment of intestinal bacteria and yeast †" it is important to start low and go slow. What this means is be patient with your approach as you implement more remedies. You can also increase the dosage of a supplement or herbal remedy overtime as the treatment process moves forward. > > It is highly recommended that you have some testing done for your child - minimally an Organic Acid Test and Comprehensive Digestive Stool Analysis (see Lab Ordering Section) - prior to attempting this protocol. The reason is you want to try and identify particular gut pathogens so you have a better idea of what to use in the protocol. > > Also, if you suspect that your child is constipated or has a sluggish bowel than it is advisable to have this evaluated before implementing this program. > > > Quote Link to comment Share on other sites More sharing options...
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