Potential viral pathogenic mechanism for new variant inflammatory bowel disease

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Mol Pathol 2002 Apr;55(2):84-90 Books,

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Potential viral pathogenic mechanism for new variant inflammatory

bowel disease.

Uhlmann V, CM, Sheils O, Pilkington L, Silva I, Killalea A,

Murch SB, - J, Thomson M, Wakefield AJ, O'Leary JJ.

Department of Pathology, Coombe Women's Hospital, Dublin 8, Ireland

Department of Histopathology, Trinity College, Dublin, Ireland,

Department of Paediatric Gastroenterology, the Royal Free Hospital,

London, UK Department of Medicine, The Royal Free Hospital and

University College Medical School, London, UK.

Aims: A new form of inflammatory bowel disease (ileocolonic

lymphonodular hyperplasia) has been described in a cohort of

children with developmental disorder. This study investigates the

presence of persistent measles virus in the intestinal tissue of

these patients (new variant inflammatory bowel disease) and a series

of controls by molecular analysis. Methods: Formalin fixed, paraffin

wax embedded and fresh frozen biopsies from the terminal ileum were

examined from affected children and histological normal controls.

The measles virus Fusion (F) and Haemagglutinin (H) genes were

detected by TaqMan reverse transcription polymerase chain reaction

(RT-PCR) and the Nucleocapsid (N) gene by RT in situ PCR.

Localisation of the mRNA signal was performed using a specific

follicular dendritic cell antibody. Results: Seventy five of 91

patients with a histologically confirmed diagnosis of ileal

lymphonodular hyperplasia and enterocolitis were positive for

measles virus in their intestinal tissue compared with five of 70

control patients. Measles virus was identified within the follicular

dendritic cells and some lymphocytes in foci of reactive follicular

hyperplasia. The copy number of measles virus ranged from one to 300

000 copies/ng total RNA. Conclusions: The data confirm an

association between the presence of measles virus and gut pathology

in children with developmental disorder.

PMID: 11950955 [PubMed - as supplied by publisher]