GreeneChipPm ids pathogens

[Guest guest]

http://www.cdc.gov/ncidod/EID/13/1/73.htm

Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases

Gustavo Palacios...

Abstract

To facilitate rapid, unbiased, differential diagnosis of infectious

diseases, we designed GreeneChipPm, a panmicrobial microarray

comprising 29,455 sixty-mer oligonucleotide probes for vertebrate

viruses, bacteria, fungi, and parasites. Methods for nucleic acid

preparation, random primed PCR amplification, and labeling were

optimized to allow the sensitivity required for application with

nucleic acid extracted from clinical materials and cultured isolates.

Analysis of nasopharyngeal aspirates, blood, urine, and tissue from

persons with various infectious diseases confirmed the presence of

viruses and bacteria identified by other methods, and implicated

Plasmodium falciparum in an unexplained fatal case of hemorrhagic

feverlike disease during the Marburg hemorrhagic fever outbreak in

Angola in 2004–2005.

http://www.cidrap.umn.edu/cidrap/content/bt/bioprep/news/

dec0706chip.html

The GreeneChip is a slide with more than 29,000 probes, or short

strips of genetic material, attached. " When human fluid and tissue

samples are applied to the chip, these probes will stick to any

closely related genetic material in the samples, " the National

Institute of Allergy and Infectious Diseases (NIAID), which supported

the research, said in a news release. " This allows the rapid and

specific identification of any pathogens therein—even those related

to but genetically distinct from the ones represented on the chip. "

- Kate

[Guest guest]

Wow. That's good. Now I wonder if they'll ever stop throwing out the slides that show positive for staphs? The so-called sample contamination issue is killing us. penny Kate <KateDunlay@...> wrote: http://www.cdc.gov/ncidod/EID/13/1/73.htmPanmicrobial Oligonucleotide Array for Diagnosis of Infectious DiseasesGustavo Palacios...AbstractTo facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the

sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004–2005.http://www.cidrap.umn.edu/cidrap/content/bt/bioprep/news/ dec0706chip.htmlThe GreeneChip is a slide with more than 29,000 probes, or short strips of genetic material, attached. "When human fluid and tissue samples are applied to the chip, these probes will stick to any closely related genetic material in the samples," the National Institute of Allergy and Infectious Diseases (NIAID), which supported the research, said in a news release. "This allows

the rapid and specific identification of any pathogens therein—even those related to but genetically distinct from the ones represented on the chip."- Kate