Re: Bowen closes; new lab will use flow cytometry

[Guest guest]

You might want to check, but I recall Lyda Mattman culturing lyme

spirokets hundreds of times from the cells infected intracellular

bodies identified with the polyvalent antibody used in the Bowen Lyme

assay. In fact, I had the opportunity to see my cells lit up with that

antibody at a 1:128 dilution. I got 2 months of Doxycycline for that

lovely picture and then was cut off.

Tom Brown

>

> Barb,

> I see they are gonna use a flow cytometer with this in the future.

That

> can sort cells - it can isolate all the objects labelled with the

> antibody, and throw away the other stuff. Maybe after concentrating

a

> bunch of the stuff, they will be able to get a PCR signal out of it?

> They certainly should be able to.

>

> Seems the close-down comes because Dr Whitaker of Bowen is mostly in

> bed with back problems... sorry news to hear.

>

> (Source for all this is Eurolyme.)

>

[Guest guest]

I too would have to check, but I remember a statement about her

performing hundreds of cultures against the qRIBb, and they all worked.

Don't remember anything being said about any negative controls, or how

she ID'd the cultures.

The message on eurolyme said Dr Whitaker was working on some papers.

It'd be great to see something come out.

>

> You might want to check, but I recall Lyda Mattman culturing lyme

> spirokets hundreds of times from the cells infected intracellular

> bodies identified with the polyvalent antibody used in the Bowen Lyme

> assay. In fact, I had the opportunity to see my cells lit up with

that

> antibody at a 1:128 dilution. I got 2 months of Doxycycline for that

> lovely picture and then was cut off.

>

> Tom Brown

>

[Guest guest]

It's not the same assay as Lida used.. that was part of the

controvery in the first place.

Barb

> >

> > Barb,

> > I see they are gonna use a flow cytometer with this in the

future.

> That

> > can sort cells - it can isolate all the objects labelled with the

> > antibody, and throw away the other stuff. Maybe after

concentrating

> a

> > bunch of the stuff, they will be able to get a PCR signal out of

it?

> > They certainly should be able to.

> >

> > Seems the close-down comes because Dr Whitaker of Bowen is mostly

in

> > bed with back problems... sorry news to hear.

> >

> > (Source for all this is Eurolyme.)

> >

>

[Guest guest]

How do you mean? I can't look it up right now, but I read somewhere

was that Mattman cultured it (how she identified it, it didn't say)

from a bunch of samples that were positive by the qRIBb. This was to

compare the qRIBb to culture, a gold standard.

> > >

> > > Barb,

> > > I see they are gonna use a flow cytometer with this in the

> future.

> > That

> > > can sort cells - it can isolate all the objects labelled with

the

> > > antibody, and throw away the other stuff. Maybe after

> concentrating

> > a

> > > bunch of the stuff, they will be able to get a PCR signal out

of

> it?

> > > They certainly should be able to.

> > >

> > > Seems the close-down comes because Dr Whitaker of Bowen is

mostly

> in

> > > bed with back problems... sorry news to hear.

> > >

> > > (Source for all this is Eurolyme.)

> > >

> >

>