Re: Tony, Fowler's experiment

[Guest guest]

That's so complicated. I would grow a lawn of staph epi and use

antibiotic discs to see what inhibited the growths and in the middle

of the plate I would put a drop of fowlers solution. Similar to the

antibiotic disc test but only missing the filt=er paper normally used

so you know the agent being tested by the markings on the paper

discs..This is the absolutely perfect test to know if you get a

response from even attempting arsenic. I found though that you could

still develop resistance easily, otherwise we'd have no argument over

the success of what tarello is all about.Arsenic distributes better

than any other agent and if used wisely in the correct mix of

treatments it would have a 90 plus % cure rate IMO..

>

> You don't have a benchtop centrifuge / microcentrifuge, do you? I

> just looked at used ones and they are not cheap, bout USD$300 for

the

> cheapest used one I could find in 2 minutes:

>

> http://www.used-line.com/c5523858s4-Eppendorf_5414.htm

>

> Anyway, if you felt like it you could probably test Fowler's on non-

> growing bacteria. It would only use up a miniscule amount of your

> Fowler's. All you do is take a colony of staph epi or whatever bug

> you want, resuspend it in a beaker of saline. That virtually stops

> their metabolism since there's no nutriment. Pour half of that

beaker

> into one tube and half into another. One tube is a control. Treat

the

> other tube with Fowler's for maybe 6 hours. Then plate out both

tubes

> on nutrient agar to see if a bunch of the treated ones died,

compared

> to the control (you count the colonies).

>

> Problem is you have to get rid of the Fowler's before plating.

> Otherwise you won't get colonies, even if they are still alive. The

> Fowler's will inhibit their growth into colonies even if it doesn't

> kill them. The only way I know how to get it out, is to centrifuge

> the bugs. That gets all the bugs into a solid pellet, so that you

can

> pour off the fluid containing Fowler's. Then you would redisolve

them

> in saline and pellet them again, a few more times, to get rid of

any

> Fowler's. (And you gotta do the same with the control.) Maybe

there's

> some other way to do it, such as filtering.

>

> I want to get me a centrifuge... probably will at some point.

>