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Vestn Ross Akad Med Nauk. 2001;(11):29-34.Links

[ultrastructural analysis as a method of studying bacteremia in

infectious diseases]

[Article in Russian]

Didenko LV.

To study bacteremic processes with transmission electron

microscopy, blood groups were examined in the representative groups of

patients with typhoid fever, generalized forms of yersinosis,

pseudotuberculosis, Flexner's shigelosis, and Sonne's shigelosis at

different stages of acute cyclic diseases and in those with chronic

Salmonella typhi carriage. Bacteremia of typical unaltered causative

organisms is shown to occur only in the feverish period of disease.

The morphofunctional organization of a causative agent in this period

is similar to that of museum bacterial strains, except that the

bacteria circulating in the blood of patients have vesicles that are

morphologically equivalent to endotoxin. In reconvalescence, the blood

circulation of causative organisms continues, but they appear as

morphologically changed bacteria and as forms with their defective

cellular wall (spheroplasts and protoplasts). Transmission electron

microscopy reveals bacteria of other systematic groups in the

patients' blood when acute Salmonella typhi carriage is under way or

when there are typhoid fever-induced complications or relapses,

clinically unfavorable running of typhoid fever, generalized forms of

yersinosis and pseudotuberculosis are present. In chronic Salmonella

typhi carriage, the patients' blood displays altered bacterial cells

and forms with defective cell wall, among them there are prominent

morphological types that are structurally identical to uncultured

bacterial forms. The study of blood samples from infected patients has

show that transmission lectron microscopy can be used to detect blood

circulating microorganisms at different stages of acute and chronic

patterns of an infectious process.

PMID: 11837203 [PubMed - indexed for MEDLINE]