New Study Sheds Light On 'Dark States' In DNA

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New Study Sheds Light On 'Dark States' In DNA

http://www.sciencedaily.com/releases/2007/01/070109142101.htm

Chemists at Ohio State University have probed an unusual high-energy

state produced in single nucleotides -- the building blocks of DNA

and RNA -- when they absorb ultraviolet (UV) light.

This is the first time scientists have been able to probe the " dark "

energy state -- so called because it cannot be detected by

fluorescence techniques used to study other high-energy states

created in DNA by UV light.

The study suggests that DNA employs a variety of means to dissipate

the energy it absorbs when bombarded by UV light.

Scientists know that UV light can cause genetic alterations that

prevent DNA from replicating properly, and these mutations can lead

to diseases such as cancer.

The faster a DNA molecule can dissipate UV energy, the lesser the

chance that it will sustain damage -- so goes the conventional

scientific wisdom. So the dark states, which are much longer lived

than previously known states created by UV light, may be linked to

DNA damage.

The existence of this dark energy state -- dubbed n(pi)*

(pronounced " n-pi-star " ) -- had previously been predicted by

calculations. Other experiments hinted at its existence, but this is

the first time it has been shown to exist in three of the five bases

of the genetic code -- cytosine, thymine and uracil.

The detection of this dark state in single bases in solution

increases the chances that it may be found in the DNA double helix,

said Bern Kohler, associate professor of chemistry at Ohio State and

head of the research team.

The Ohio State chemists determined that, when excited by ultraviolet

light, these three bases dissipate energy through the dark state

anywhere from 10-50 percent of the time.

The rest of the time, energy is dissipated through a set of energy

states that do fluoresce in the lab. These " bright " energy states

dissipate the energy much faster, in less than one picosecond.

A picosecond is one millionth of one millionth of a second -- an

inconceivably short length of time. Light travels at 186,000 miles

per second, but in twenty picoseconds it would only travel just

under a quarter of an inch. Still, a picosecond is not so fast

compared to the speed of some chemical reactions in living cells.

In tests of single DNA bases, the dark state lasted for 10-150

picoseconds -- much longer than the bright state. The chemists

reported their results in the Proceedings of the National Academy of

Sciences.

" We want to know, what makes DNA resist damage by UV light? " said

Kohler. " In 2000, we showed that single DNA bases can dissipate UV

energy in less than one picosecond. But now we know that there are

other energy states that have relatively long lifetimes. "

" Now we see that there is a family of energy states in DNA

responsible for energy dissipation, and this is a major correction

in how we view DNA photostability. "

Until now, the proposed dark energy state of DNA was a little like

the dark matter in the universe – there was no direct way of probing

it. The Ohio State chemists used a technique called transient

absorption, which is based on the idea that molecules absorb light

at specific wavelengths, and allows them to study events happening

in less than a picosecond.

They found that DNA dissipates UV energy through the dark state 10-

50 percent of the time, depending on which DNA base is excited, and

whether a sugar molecule is attached to the base or not.

Next, Kohler's lab is investigating whether the dark state can be

linked to DNA damage.

" What are the photochemical consequences of long-lived states? Are

they precursors to some of the chemical photoproducts that we know

cause damage? That's the Holy Grail in this field -- connecting our

growing knowledge of the electronic states of DNA with the

photoproducts that damage it, " he said.

Kohler's coauthors include E. Crespo-, a former

postdoctoral researcher at Ohio State, and M. Hare, who just

obtained his Ph.D. from the university and is about to begin a

position as a postdoctoral researcher at the University of Notre

Dame.

Note: This story has been adapted from a news release issued by Ohio

State University.