Muscle-derived stem cells isolated as non-adherent population give rise to cardi

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Exp Cell Res. 2008 Jan 19

Muscle-derived stem cells isolated as non-adherent population give

rise to cardiac, skeletal muscle and neural lineages.

Arsic N, Mamaeva D, Lamb NJ, Fernandez A.

Cell Biology Unit, Institute for Human Genetics, CNRS, 141 rue de la

Cardonille, Montpellier, France.

Stem cells with the ability to differentiate in specialized cell

types can be extracted from a wide array of adult tissues including

skeletal muscle. Here we have analyzed a population of cells isolated

from skeletal muscle on the basis of their poor adherence on uncoated

or collagen-coated dishes that show multi-lineage differentiation in

vitro.

When analysed under proliferative conditions, these cells express

stem cell surface markers Sca-1 (65%) and Bcrp-1 (80%) but also MyoD

(15%), Neuronal beta III-tubulin (25%), GFAP (30%) or Nkx2.5 (1%).

Although capable of growing as non-attached spheres for months, when

given an appropriate matrix, these cells adhere giving rise to

skeletal muscle, neuronal and cardiac muscle cell lineages. A similar

cell population could not be isolated from either bone marrow or

cardiac tissue suggesting their specificity to skeletal muscle.

When injected into damaged muscle, these non-adherent muscle-derived

cells are retrieved expressing Pax7, in a sublaminar position

characterizing satellite cells and participate in forming new

myofibers. These data show that a non-adherent stem cell population

can be specifically isolated and expanded from skeletal muscle and

upon attachment to a matrix spontaneously differentiate into muscle,

cardiac and neuronal lineages in vitro.

Although competing with resident satellite cells, these cells are

shown to significantly contribute to repair of injured muscle in vivo

supporting that a similar muscle-derived non-adherent cell population

from human muscle may be useful in treatment of neuromuscular

disorders.