Guest guest Posted February 27, 2006 Report Share Posted February 27, 2006 DCC017001517 WEST COAST ANALYTICAL SERVICES November 2O, 1992 BRODY, M.D. INC. GARRY S hire Medical Building #504 11411 hire Avenue Downey, California 90241 Attn: Dr. Garry Brody JOB NO. 22488 LABORATORY REPORT Samples Received: One (1) Breast Milk Sample Date Received: 9-30-92 Purchase Order No: COD The sample was analyzed as follows: Samples Analyzed Analysis Results One (1) Breast Milk Silicone by GC/FID Table 1 The sample was analyzed using modification of the procedure developed by Dow Chemical for silicon. (po1ydimethy1silaxane) in water. The sample is reacted with hydrochloric acid to hydrolyze the silicone to dimethyldisilanol, than with hexamethyldisiloxane (HMDS) to generate dimethoxydimethlsilane (D). This compound is then determined by gas chromatography (GC). The sample results are shown in Table 1. The detection limit is high because of an interference in the method blank which was shown to be due to D in the reagents. This was shown using both QC and gas chromatography-mass spectrometry (GCMS). Attempts to eliminate this interference by distilling the HMDS failed. No other interferences from the matrix were observed. Bettina Oelke Senior Chemist Shelton Technical Director This report to be reproduced in its entirety. ============================================= GARRY S. BRODY, M.D., INC. Job # 22488 Dr. Garry Brady November 20, 1992 LABORATORY REPORT The chemical used in this procedure for calibration is dichlorodimethylsilane (DCDS). The silicone gel used in implants could not be dissolved in any solvent, so calibration solutions could not be made from the gel. An attempt to disperse the gel in both homogenized milk and whole cream using ultrasonication also failed. A sample of the gel, unweighed, was taken successfully through the analytical procedure showing that the test method is valid. In conclusion, the method appears to work, but it appears that it is impossible to disperse or dissolve the silicone gel in body fluids in high enough concentrations to be measured. Dow developed the original test for low molecular weight silicones, used as anti-foams-and surfactants in water treatment, forms that are-water soluble. Perhaps the test could be applied to tissues, however the presence of silicone in tissues may be obvious from the situation. Since silicone gel is not soluble in body fluids above 5ppm it may be unlikely to metabolize fast enough to show an elemental silicon. The rate of elimination is probably faster than the rate of metabolization. While these results are negative, do you have any suggestions for further research or where and how these results could be published? =============================== WEST COAST ANALYTICAL SYSTEMS GARRY S. BRODY, M.D. * INC Dr. Carry Brady LABORATORY REPORT Job #22488 November 20, 1992 Table 1 Silicone by GC/FID Sample ID Parts per million (mg/l) Breast Milk, Chase D. L. N. D. Detection Limit *High detection limit due to an interference in blank. Date extracted: 10/2/92 Date Analyzed: 10/6/92 Quote Link to comment Share on other sites More sharing options...
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