Oxford Journals Life Sciences Carcinogenesis Volume 9

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ingested AFB1 becomes covalently bound to serum albumin, a value

very similar to that observed when rats are administered AFB1.

Oxford Journals Life Sciences Carcinogenesis Volume 9, Number 7 Pp.

1323-1325

http://carcin.oxfordjournals.org/cgi/content/abstract/9/7/1323

Articles by Gan, L.-S.

Articles by Tannenbaum, S. R.

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Articles by Gan, L.-S.

Articles by Tannenbaum, S. R.

© 1988 Oxford University Press

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Serum albumin adducts in the molecular epidemiology of aflatoxin

carcinogenesis: correlation with aflatoxin B1 intake and urinary

excretion of aflatoxin M1

Liang-Shang Gan, L. Skipper, Xiaocong Peng, D. Groopman 1,

Jun-shi Chen 2, Gerald N. Wogan and R. Tannenbaum

Department of Applied Biological Sciences, Massachusetts Institute

of Technology Cambridge, MA 02178

1Boston University School of Public Health Boston, MA 02118, USA

2Institute of Nutrition and Food Hygiene, Chinese Academy of

Preventive Medicine Beijing, China

Aflatoxin-serum albumin adducts in the blood of 42 residents of

Guangxi Province, People's Republic of China, were determined and

compared with intake of aflatoxin B1 (AFB1) and excretion of

aflatoxin M1 (AFM1) in urine. Blood specimens were obtained during

the same period that urine was collected and that diet was sampled.

Serum albumin was isolated from blood by affinity chromatography on

Reactive Blue 2-Sepharose and subjected to enzymatic proteolysis

using Pronase. Immunoreactive products were purified by

immunoaffinity chromatography and quantified by competitive

radioimmunoassay. A highly significant correlation (r = 0.60, P <

0.00003) of adduct level with AFM, excretion was observed. An

equally highly significant correlation of adduct level with intake

(r = 0.69, P < 0.000001) was also observed. From the slope of the

regression line for adduct level as a function of intake, it was

calculated that 1.4–2.3% of ingested AFB1 becomes covalently bound

to serum albumin, a value very similar to that observed when rats

are administered AFB1.

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