Re: Re: Can you save personal papers?

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Carl and Stan.

This paper is highly relevant and important to the implications of this

discussion.

Mycopathologia. <javascript:AL_get(this, 'jour', 'Mycopathologia.');> 2004

Jul;158(1):87-97. <javascript:PopUpMenu2_Set(Menu15487326);>

Protein translation inhibition by Stachybotrys chartarum conidia with and

without the mycotoxin containing polysaccharide matrix.*Karunasena

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*Cooley

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*Straus

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*Straus

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..

Department of Microbiology and Immunology, Texas Tech University Health

Sciences Center, Lubbock, TX 79430, USA.

Recent studies have correlated the presence of Stachybotrys chartarum in

structures with SBS. S. chartarum produces mycotoxins that are thought to

produce some of the symptoms reported in sick-building syndrome (SBS). The

conidia (spores) produced by Stachybotrys species are not commonly found in

the air of buildings that have been found to contain significant interior

growth of this organism. This could be due in part to the large size of the

Stachybotrys spores, or the organism growing in hidden areas such as wall

cavities. However, individuals in buildings with significant Stachybotrys

growth frequently display symptoms that may be attributed to exposure to the

organism's mycotoxins. In addition, Stachybotrys colonies produce a " slime "

or polysaccharide (carbohydrate) matrix that coats the hyphae and the

spores. The intent of this project was to determine whether the carbohydrate

matrix and the mycotoxins embedded in it could be removed from the spores by

repeated washings with either aqueous or organic solvents. The results

demonstrated that the process of spore washing removed compounds that were

toxic in a protein translation assay as compared to spores that were washed

with an organic solution, however a correlation between carbohydrate removal

during the washing process and the removal of mycotoxins from the spore

surface was not observed. These data demonstrated that mycotoxins are not

likely to be found exclusively in the carbohydrate matrix of the spores.

Therefore, mycotoxin removal from the spore surface can occur without

significant loss of polysaccharide. We also showed that toxic substances may

be removed from the spore surface with an aqueous solution. These results

suggest that satratoxins are soluble in aqueous solutions without being

bound to water-soluble moieties, such as the carbohydrate slime matrix.

PMID: 15487326 [PubMed - indexed for MEDLINE]