Transcriptomic analysis of the exit from dormancy of Aspergillus fumigatus conid

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Transcriptomic analysis of the exit from dormancy of Aspergillus

fumigatus conidia

7thSpace Interactive (press release) - New York,NY*

http://7thspace.com/headlines/293013/transcriptomic_analysis_of_the_e

xit_from_dormancy_of_aspergillus_fumigatus_conidia.html

Establishment of aspergillosis is depending upon the exit from

dormancy and germination of the conidia of Aspergillus fumigatus in

the lung. To gain an understanding of the molecular mechanisms

underlying the early steps of conidial germination, we undertook a

transcriptomic analysis using macroarrays constructed with PCR

fragments from >3,000 genes (around one third of the annotated A.

fumigatus genome).

Results: Major results of this analysis are the following: (i)

conidia stored pre-packaged mRNAs transcripts (27% of genes have

transcripts in the resting conidia; (ii) incubation at 37degreesC in

a nutritive medium induced up- and down-regulation of genes: 19% of

the total number of genes deposited on the array were up-regulated

whereas 22% of the genes with pre-packaged mRNA in the resting

conidia were down-regulated; (iii) most modifications were seen

during the first 30 min of germination whereas very little

modification of gene expression occurred during the following hour;

(iv) one-year old conidia and one-week old conidia behaved similarly

at transcriptional level.

Conclusions: Transcriptomic data indicate that the exit from

dormancy is associated with a shift from a fermentative metabolism

to a respiratory metabolism as well as a trend toward immediate

protein synthesis.

Author: Claude Lamarre, Serguei Sokol, Jean- Debeaupuis,

Henry, Celine Lacroix, Philippe Glaser, Jean-Yves Coppee,

Jean-Marie Francois and Jean- Latge

Credits/Source: BMC Genomics 2008, 9:417

[Guest guest]

what does this mean as far as if your colonized? that intake of sugar

or alcohol or even the smell what activate it?

--- In , " tigerpaw2c " <tigerpaw2c@...>

wrote:

>

> Transcriptomic analysis of the exit from dormancy of Aspergillus

> fumigatus conidia

> 7thSpace Interactive (press release) - New York,NY*

>

>

http://7thspace.com/headlines/293013/transcriptomic_analysis_of_the_e

> xit_from_dormancy_of_aspergillus_fumigatus_conidia.html

>

> Establishment of aspergillosis is depending upon the exit from

> dormancy and germination of the conidia of Aspergillus fumigatus in

> the lung. To gain an understanding of the molecular mechanisms

> underlying the early steps of conidial germination, we undertook a

> transcriptomic analysis using macroarrays constructed with PCR

> fragments from >3,000 genes (around one third of the annotated A.

>

> fumigatus genome).

>

> Results: Major results of this analysis are the following: (i)

> conidia stored pre-packaged mRNAs transcripts (27% of genes have

> transcripts in the resting conidia; (ii) incubation at 37degreesC

in

> a nutritive medium induced up- and down-regulation of genes: 19% of

> the total number of genes deposited on the array were up-regulated

> whereas 22% of the genes with pre-packaged mRNA in the resting

> conidia were down-regulated; (iii) most modifications were seen

> during the first 30 min of germination whereas very little

> modification of gene expression occurred during the following hour;

> (iv) one-year old conidia and one-week old conidia behaved

similarly

> at transcriptional level.

>

> Conclusions: Transcriptomic data indicate that the exit from

> dormancy is associated with a shift from a fermentative metabolism

> to a respiratory metabolism as well as a trend toward immediate

> protein synthesis.

>

> Author: Claude Lamarre, Serguei Sokol, Jean- Debeaupuis,

> Henry, Celine Lacroix, Philippe Glaser, Jean-Yves Coppee,

> Jean-Marie Francois and Jean- Latge

> Credits/Source: BMC Genomics 2008, 9:417

>