Mycotoxin Detection in Human Samples from Patients Exposed to Environmental Mold

[Guest guest]

Int. J. Mol. Sci. 2009, 10(4), 1465-1475; doi:10.3390/ijms10041465

Article

Mycotoxin Detection in Human Samples from Patients Exposed to Environmental

Molds

http://www.mdpi.com/1422-0067/10/4/1465

Dennis G. Hooper 1,* , E. Bolton 1, Frederick T. Guilford 2 and C.

Straus 3

1 RealTime Laboratories, LLC, 13016 Bee Street #203, Dallas, TX 79234, USA

2 5050 El Camino Real, #110, Los Altos, CA 94022, USA

3 Department of Microbiology and Immunology, Texas Tech University Health

Sciences Center, Lubbock, TX 79430, USA

* Author to whom correspondence should be addressed.

Received: 4 February 2009; in revised form: 13 March 2009 / Accepted: 27 March

2009 / Published: 1 April 2009

This article belongs to the special issue Mycotoxins: Mechanisms of

Toxicological Activity - Treatment and Prevention

Download PDF Full-Text (85 KB)

Abstract: The goal of this study was to determine if selected mycotoxins

(trichothecenes, aflatoxins, and ochratoxins) could be extracted and identified

in human tissue and body fluids from patients exposed to toxin producing molds

in their environment. Human urine and methanol extracted tissues and sputum were

examined. Trichothecenes were tested using competitive ELISA techniques.

Aflatoxins B1, B2, G1, and G2, and ochratoxin A were tested by using

immunoaffinity columns and fluorometry. Test sensitivity and specificity were

determined. Levels of detection for the various mycotoxins varied from 0.2 ppb

for trichothecenes, 1.0 ppb for aflatoxins, and 2.0 ppb for ochratoxins.

Trichothecene levels varied in urine, sputum, and tissue biopsies (lung, liver,

brain) from undetectable (<0.2 ppb) to levels up to 18 ppb. Aflatoxin levels

from the same types of tissues varied from 1.0 to 5.0 ppb. Ochratoxins isolated

in the same type of tissues varied from 2.0 ppb to > 10.0 ppb. Negative control

patients had no detectable mycotoxins in their tissues or fluids. These data

show that mycotoxins can be detected in body fluids and human tissue from

patients exposed to mycotoxin producing molds in the environment, and

demonstrate which human tissues or fluids are the most likely to yield positive

results.

Keywords: Aflatoxin; ochratoxin; trichothecene; human samples

[Guest guest]

Here's the link and I attached a copy of the full report.

http://www.mdpi.com/1422-0067/10/4/1465

________________________________

From: dragonflymcs <dragonflymcs@...>

Sent: Thursday, September 10, 2009 10:48:18 AM

Subject: Re: [] Mycotoxin Detection in Human Samples from Patients

Exposed to Environmental Mold

Can you please submit his link again, TY

God Bless !!

dragonflymcs

Mayleen