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O/T Researchers Develop GPC-LC-MS/MS Method to Screen for 31 Mycotoxins in Edibl

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PRESS RELEASE

MarketWatch (press release) - USA

Researchers Develop GPC-LC-MS/MS Method to Screen for 31 Mycotoxins

in Edible Oil

Comprehensive Mycotoxin Screen Enables Compliance with New

Regulations at Lower Cost

http://www.marketwatch.com/news/story/researchers-develop-gpc-lc-msms-

method-screen/story.aspx?guid=%7B77CFFA93-EE7C-4528-95C6-2D2D4D642A1A%

7D & dist=msr_1

Last update: 10:26 a.m. EST Feb. 25, 2009

MOUNTAIN VIEW, Calif., Feb 25, 2009 (BUSINESS WIRE) -- Pickering

Laboratories today announced that researchers at the Munich Technical

University and Bavarian Health and Food Safety Authority have

developed an innovative, comprehensive mycotoxin screening method

based on the use of LCTech's GPC ULTRA gel permeation chromatography

instrument. Distributed by Pickering Laboratories in North America

for sample cleanup prior to separation and detection, the new GPC

instrument separates low weight mycotoxins from high molecular weight

food constituents prior to high performance liquid chromatography

(HPLC) separation and mass spectroscopy (MS) detection. The new

method substantially lowers the cost of performing a comprehensive

mycotoxin screen by detecting 31 mycotoxins in a single analysis run.

A proposed European Community regulation sets a maximum level of

1000ug/kg for fumonisins (FB1/FB2) in corn oil, making it more

important than ever to develop a comprehensive mycotoxin screen.

Development of a comprehensive screen is a demanding challenge

because of the wide range of chemical and physical properties of the

individual mycotoxins and the large number of naturally occurring

food constituents that may be extracted along with the mycotoxins and

interfere with separation and detection.

Traditional methods for detecting and quantifying low levels of

mycotoxins are specific to individual mycotoxins, so it is time-

consuming, difficult and expensive to carry out a comprehensive

screen. Earlier GPC-based methods have been based on commonly-used

polystyrene polymer beads, which are suitable for zeralenone (ZEA),

ochratoxin A (OTA), aflatoxins and trichothecenes. However, this

material does not provide sufficient selectivity to separate

fumonisins (FB1/FB2) from the oil fraction.

C. Gottshalk and J. Bauer of Munich Technical University, J. Barthel

and G. Engelhardt of the Bavarian Health and Food Safety Authority

and U. Aulwurm of LCTech worked together to develop a method to

detect 31 different mycotoxins, including ZEA, fumonisins,

trichothecenes (types A, B and D), aflatoxins, ochratoxin A and other

mycotoxins, from edible oils. The new method relies on the fact that

mycotoxins fall into the 200 to 600 molecular weight range and thus

can be separated using GPC from food constituents that have much

higher molecular weights.

To develop this comprehensive screen, researchers used the LCTech's

GPC ULTRA automated sample cleanup and concentration instrument,

which is distributed by Pickering Laboratories in Canada, Mexico, and

the United States. They tried a range of different columns and found

that the LCTech's MykoClean column successfully separated all 31

analytes from the oil. The researchers studied influencing factors

such as the eluent composition, pH, temperature and the column

loading capacity. The recoveries obtained with the combined GPC-LC-

MS/MS method range between 74% and 104%. The limits of quantification

achieved with the new method comply with the maximum levels for

analytes regulated by EC 1881/2006 regulations from December 19,

2006.

For additional detail, please see the poster at

http://www.pickeringlabs.com/pdf/080425-ua-

Poster_30_Mykotoxin_Workshop1.pdf

About the GPC ULTRA

The GPC ULTRA is designed for removing fats, proteins, and other

large bio-molecules from samples to prevent interference in the

analysis of mycotoxins, chlorinated hydrocarbon pesticides,

polychlorinated biphenyls (PCBs), antibiotics, polycyclic aromatic

hydrocarbons (PAHs) and other semi-volatile compounds. The GPC ULTRA

features a unique rotary evaporation chamber that combines a silicon

oil bath and precise heat transfer with a laser- directed volume

control for precision evaporation of solvents. It also includes an

auto sampler, precision pumps and valve injection system, a sample

loop and a matched GPC column. Vario PC software controls and

monitors the instrument's operation. (For additional detail, see

http://www.pickeringlabs.com/catalog/sample_preparation_GPC_ULTRA.htm)

About LCTech

LCTech GmbH, based in the Bavarian town of Dorfen, develops and

distributes innovative products and methods for the preparation and

analysis of food, feed and environmental samples. The range of

products covers GPC systems as well as solutions for the analysis of

mycotoxins. Worldwide, LCTech is represented by a well-developed

distributor network

Globally, public and private laboratories in the food and feed

industries and in pharmacy and research rely on the modern and

efficient products and individual service guaranteed by LCTech. For

further information, please visit www.LCTech.de

About Pickering Laboratories

Pickering Laboratories is a pioneer in biochemical reagents and

instrumentation. For 25 years, Pickering has developed applications

required by government and private laboratories. Pickering leadership

in both the design and manufacture of biochemical reagents and

instrumentation assures the quality of both. With the addition of

LCTech mycotoxin products to the company's product line, Pickering

now enables customers to analyze and process mycotoxins with more

tools from a known source of quality products and excellent service.

Pickering's team of dedicated research and application chemists,

engineers, operational specialists, and experienced managers produce

and sell direct and through 87 distributors in 150 countries in North

and South America, Europe, the Middle East, Africa, Asia, and the

Caribbean. Headquarters are in Mountain View, California. For

additional information, please visit www.pickeringlabs.com

Note to Editors:

For a poster describing the research above, please see

http://www.pickeringlabs.com/pdf/080425-ua-

Poster_30_Mykotoxin_Workshop1.pdf

Graphics and images from the poster are available upon request.

Please contact Marchant at 919-451-0776 or

linda.marchant@...

SOURCE: Pickering Laboratories

Pickering Laboratories, Inc.

Mike Gottschalk, 800-654-3330 or 650-694-6700

mgottschalk@...

or

Cayenne Communication

Marchant, 919-451-0776

linda.marchant@...

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