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CHRONIC INFLAMATION,

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Pathobiology of Inflammation and Repair

Part 3: Chronic Inflammation and Repair

go over chart on p. 74

Chronic Inflammation

Basic Features

prolonged, actively inflammed, tissue destruction, and attempts at healing

Setting for chronic inflammation

persistent infections- granulomatous formation

prolonged exposure to toxic agents

autoimmune diseases

Characterized by:

infiltration with mononuclear cells- macs, lymphs, and plasma cells

tissue destruction

angiogenesis and fibrogenesis

Mononuclear infiltration

major player is tissue macrophage

Mononuclear phagocyte system (RE system)

monocytes begin to migrate early in acute inflammation, and are predominant cell

type in 48 hours.

macs are activated by cytokines

Products of these macrophages are involved in tissue injury and fibrosis

Three mechanisms of macrophage accumulation

continued recruitment of monocytes from the circulation by continued expression

of adhesion molecules and chemotactic factors C5a, IL-8, etc.

local proliferation of macrophages

immobilization of macs at target site

other cells involved include lymphs, plasma cells, eosinophils, and mast cells

Fibrosis (repair by connective tissue)

attempts at repair lead to scarring and fibrosis

Four components of process

angiogenesis

migration and proliferation of fibroblasts

deposition of extracellular matrix

remodeling

Granulation tissue is angiogenesis and fibrosis

Granulomatous inflammation

distinctive pattern of chronic inflammatory response in which the predominant

cell type is an activated macrophage with a modified (epitheloid) appearance

Granulomas are small round collections of macrophages surrounded by a rim of

lymphocytes

Two types: Foreign body granulomas and immune granulomas

Granulomatous inflammation occurs with: TB, sarcoidosis, leprosy, cat scratch

fever, schistosomiasis, syphilis, deep fungal diseases, inorganic metals and

dusts

Can be caseating or non-caseating

Inability to digest inciting agent + CMI

Repair

Two distinct processes may be involved:

Regeneration- replacement of dead tissue by parenchymal cells of the same type

Replacement- connective tissue stroma (scar formation)

Chronology of the Repair process

Removal of the inflammatory exudate

36 hrs to 3-4 weeks

begins with migration of macrophages

Regeneration by parenchyma or fibrous proliferation

type of replacement depends on the type of tissue injured

begins within a few hours

Factors influencing the repair process

Types of cells

Labile cells- continue to multiply throughout life

Stabile cells- contain a latent capacity to regenerate, but under normal

conditions do not undergo mitotic activity

Permanent Cells- no regenerative capacity (ex. cardiac myocytes and neurons)

Repair by connective tissue

Sequence of scar formation

fibroblasts and endothelial proliferation

begins as early as 24 hours and migration along fibrin strands begins within a

few days

Collagen is detectable in 4 days

increase in collagen leads to increased wound strength

progressive devascularization ( weeks to months )

Integration of Parenchymal and Connective Tissue Healing

Wound Healing

Healing by first intention (primary union)

ideal healing

occurs in a clean wound when the edges have been approximated

Healing by second intention (secondary union)

extensive tissue loss

dead tissue must be removed before healing can occur

contraction of collagen after healing has occurred causes depression at the scar

site

Stimulation of Cell Proliferation

No one knows how cell proliferation is initiated.

see areas of research on p 71-2

Wound Strength

Collagen is the ultimate source of strength of the healed wound

Factors which modify the Inflammatory-Reparative Response

age is not a factor

A severe protein depletion impairs wound healing

Vit C is important in formation of collagen

hydrocortisones inhibit inflammatory reaction

the better the blood supply, the more resistant to infection

immobilization is important in healing of bone and excessive hemorrage

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