CFS, MS & Healthy Controls -Differences After Moderate Exercise

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http://www.ncbi.nlm.nih.gov/pubmed/22210239

PubMed.gov

US National Library of Medicine National Institutes of Health

Psychosom Med. 2011 Dec 30. [Epub ahead of print]

Differences in Metabolite-Detecting,

Adrenergic, and Immune Gene

Expression After Moderate Exercise

in Patients With Chronic Fatigue

Syndrome, Patients With Multiple

Sclerosis, and Healthy Controls.

White AT, Light AR, Hughen RW, Vanhaitsma

TA, Light KC.

Departments of Exercise and Sport Science (A.T.W.,

T.A.V.), Anesthesiology (A.R.L., R.W.H., K.C.L.), and

Neuroscience (A.R.L.) and The Brain Institute (A.T.W.),

University of Utah, Salt Lake City, Utah.

Abstract

Objective

Chronic fatigue syndrome (CFS) and multiple sclerosis (MS)

are characterized by debilitating fatigue, yet evaluation of

this symptom is subjective.

We examined metabolite-detecting, adrenergic, and immune

gene expression (messenger ribonucleic acid [mRNA]) in

patients with CFS (n = 22) versus patients with MS (n = 20)

versus healthy controls (n = 23) and determined their

relationship to fatigue and pain before and after exercise.

Methods

Blood samples and fatigue and pain ratings were obtained at

baseline and 0.5, 8, 24, and 48 hours after sustained

moderate exercise.

Leukocyte mRNA of four metabolite-detecting receptors

(acid-sensing ion channel 3, purinergic type 2X4 and 2X5

receptors, and transient receptor potential vanilloid type 1)

and four adrenergic (a-2a, beta-1, and beta-2 receptors and

catechol-O-methyltransferase) and five immune markers

(CD14, toll-like receptor 4 [TLR4], interleukin [iL] 6, IL-10,

and lymphotoxin a) was examined using quantitative

polymerase chain reaction.

Results

Patients with CFS had greater postexercise increases in

fatigue and pain (10-29 points above baseline, p < .001) and

greater mRNA increases in purinergic type 2X4 receptor,

transient receptor potential vanilloid type 1, CD14, and all

adrenergic receptors than controls (mean ± standard error =

1.3 ± 0.14- to 3.4 ± 0.90-fold increase above baseline, p =

..04-.005).

Patients with CFS with comorbid fibromyalgia (n = 18) also

showed greater increases in acid-sensing ion channel 3 and

purinergic type 2X5 receptors (p < .05).

Patients with MS had greater postexercise increases than

controls in beta-1 and beta-2 adrenergic receptor

expressions (1.4 ± 0.27- and 1.3 ± 0.06-fold increases,

respectively, p = .02 and p < .001) and greater decreases in

TLR4 (p = .02). In MS, IL-10 and TLR4 decreases correlated

with higher fatigue scores.

Conclusions

Postexercise mRNA increases in metabolite-detecting

receptors were unique to patients with CFS, whereas both

patients with MS and patients with CFS showed abnormal

increases in adrenergic receptors.

Among patients with MS, greater fatigue was correlated with

blunted immune marker expression.

PMID: 22210239 [PubMed - as supplied by publisher]