AW: EN>DE pure multiplex testing (urgent)

[Guest guest]

Dear ,

Yes, very helpful and in fact it confirms my own hunch about the meaning.

Thanks a bunch.

Astrid

-----Ursprüngliche Nachricht-----

Von: medical_translation

[mailto:medical_translation ] Im Auftrag von cgtradmed

Gesendet: Tuesday, May 22, 2012 11:02 AM

An: medical_translation

Betreff: Re: EN>DE pure multiplex testing (urgent)

Hi Astrid,

Can this help you ?:

" Host: Keeping in mind the strengths and weaknesses of multiplexed platforms

is there a maximum number of tests that can practically be multiplexed

together, Dr. Klee?

Dr. Klee: That is a very difficult question, if you look at the

gnomic testing, they're doing thousands of them, you're looking at some of

these array type things where they're doing hundreds, these issues that I

just alluded to in terms of false positives or optimization of assays, I

think become overwhelming.

My recommendation would be to keep it down to an accountable number; if you

count with your fingers say ten, if you only want to count on one hand,

five. Somewhere in that range I think is doable with these multiplexed

assays, but once you get much higher than that, I think these other issues

of optimization, of quality control, and another thing we didn't even talk

about is cross reactivity between the different substances that are being

tested in that same sample.

So I think there’s some major issues once you get beyond ten analytes that

you're trying to measure in one particular well.

Host: Well, lastly, Dr. Mater, based on your experience what advice would

you give to those who are developing multiplexed devices?

Dr. Master: Well, I think there are several key lessons at this

point, and the first is to know your question, because this is going to

fundamentally affect the number of analytes that are chosen. So knowing the

disease and the kind of answer you expect out of your device, I think, is

going to be critical. ***On the one hand you might be focusing on individual

analytes as a pure multiplex panel, and on the other hand you might be

looking at a pattern as a whole.*** "

In : Measurement and Quality Control in Protein Microassays :

http://www.aacc.org/events/podcast/Documents/082809Master.pdf

Le 22/05/2012 10:45, Astrid Kaeser a écrit :

> Dear all,

>

> I am editing a questionnaire on the use of biomarkers in oncology and

> stumbling over the terms (left in English by the translator):

> - panel based multiplex

> - pure multiplex testing

>

> As for panel based multiplex tests I understand that they are based on

> a panel of markers, but I am struggling with the meaning of " pure

> multiplex testing " and could not find an appropriate explanation on the

internet.

>

> Many thanks for your help in advance

> Astrid

>

>

>

>

>

> ------------------------------------

>

> URL: http://groups.yahoo.com/group/medical_translation

>

> In case of any problem with this list, you can reach the moderator at

cgtradmed@....

> The FAQs of our list are available at :

> http://groups.yahoo.com/group/medical_translation/files/M_T-FAQS.doc

>

> To unsubscribe, please send an *empty* message to

> medical_translation-UNSUBSCRIBE@...! Groups Links

>

>

>

>

>

------------------------------------

URL: http://groups.yahoo.com/group/medical_translation

In case of any problem with this list, you can reach the moderator at

cgtradmed@....

The FAQs of our list are available at :

http://groups.yahoo.com/group/medical_translation/files/M_T-FAQS.doc

To unsubscribe, please send an *empty* message to

medical_translation-UNSUBSCRIBE@...! Groups Links