EN>DE pure multiplex testing (urgent)

[Guest guest]

Dear all,

I am editing a questionnaire on the use of biomarkers in oncology and

stumbling over the terms (left in English by the translator):

- panel based multiplex

- pure multiplex testing

As for panel based multiplex tests I understand that they are based on a

panel of markers, but I am struggling with the meaning of " pure multiplex

testing " and could not find an appropriate explanation on the internet.

Many thanks for your help in advance

Astrid

[Guest guest]

Hi Astrid,

Can this help you ?:

" Host: Keeping in mind the strengths and weaknesses of

multiplexed platforms is there a maximum number of tests

that can practically be multiplexed together, Dr. Klee?

Dr. Klee: That is a very difficult question, if you look at the

gnomic

testing, they're doing thousands of them, you're looking at

some of these array type things where they're doing

hundreds, these issues that I just alluded to in terms of

false positives or optimization of assays, I think become

overwhelming.

My recommendation would be to keep it down to an

accountable number; if you count with your fingers say ten,

if you only want to count on one hand, five. Somewhere in

that range I think is doable with these multiplexed assays,

but once you get much higher than that, I think these other

issues of optimization, of quality control, and another thing

we didn't even talk about is cross reactivity between the

different substances that are being tested in that same

sample.

So I think there’s some major issues once you get beyond

ten analytes that you're trying to measure in one particular

well.

Host: Well, lastly, Dr. Mater, based on your experience what

advice would you give to those who are developing

multiplexed devices?

Dr. Master: Well, I think there are several key lessons at this

point, and

the first is to know your question, because this is going to

fundamentally affect the number of analytes that are

chosen. So knowing the disease and the kind of answer you

expect out of your device, I think, is going to be critical. ***On

the one hand you might be focusing on individual analytes

as a pure multiplex panel, and on the other hand you might

be looking at a pattern as a whole.*** "

In : Measurement and Quality Control in Protein Microassays :

http://www.aacc.org/events/podcast/Documents/082809Master.pdf

Le 22/05/2012 10:45, Astrid Kaeser a écrit :

> Dear all,

>

> I am editing a questionnaire on the use of biomarkers in oncology and

> stumbling over the terms (left in English by the translator):

> - panel based multiplex

> - pure multiplex testing

>

> As for panel based multiplex tests I understand that they are based on a

> panel of markers, but I am struggling with the meaning of " pure multiplex

> testing " and could not find an appropriate explanation on the internet.

>

> Many thanks for your help in advance

> Astrid

>

>

>

>

>

> ------------------------------------

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