MMP profile in paired serum and synovial fluid samples of patients with RA

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Rheum Dis. 2004 Jul;63(7):881-3.

MMP profile in paired serum and synovial fluid samples of patients with

rheumatoid arthritis.

Tchetverikov I, Ronday HK, Van El B, Kiers GH, Verzijl N, TeKoppele JM,

Huizinga TW, DeGroot J, Hanemaaijer R.

TNO Prevention and Health, PO Box 2215, 2301 CE, Leiden, The

Netherlands. R.Hanemaaijer@...

OBJECTIVE: To analyse matrix metalloproteinases (MMPs) and tissue

inhibitor-1 of MMPs (TIMP-1) levels in the systemic circulation and

synovial fluid (SF) of patients with RA and to compare these levels with

inflammatory and collagen degradation markers. METHODS:

ProMMP-1, -2, -3, -8, -9, TIMP-1, levels of MMP/alpha(2)-macroglobulin

complexes, and collagen degradation products were measured by sandwich

ELISA, activity assays, and HPLC in paired SF and serum samples from 15

patients with RA and 13 with OA. RESULTS: MMPs were higher in SF of

patients with RA than in OA or controls. MMP levels in SF of patients

with OA were higher than in controls. In serum, levels of proMMP-3, -8

and -9 were higher in patients with RA than in OA or controls, whereas

only proMMP-8 and -9 were higher in serum of patients with OA than in

controls. A strong correlation was seen between serum and SF levels of

MMP-8 and -9 in RA. Increased levels of MMP/alpha(2)-macroglobulin

complexes indicated an MMP/TIMP imbalance in serum and SF in RA. SF

hydroxyproline correlated significantly with SF levels of proMMP-9 in

RA.

CONCLUSIONS: Systemic MMP-8 and -9 levels represent the situation in the

inflamed joint; MMP-9 is likely to be involved in degradation of joint

collagen. The hypothesis of MMP/TIMP imbalance in RA is strengthened.

PMID: 15194590

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