(mentions CMT) Differential gene expression in chronic inflammatory demyelinatin

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J Neurol Sci. 2009 Nov 16.

Differential gene expression in chronic inflammatory demyelinating

polyneuropathy (CIDP) skin biopsies.

Lee G, Xiang Z, Brannagan TH 3rd, Chin RL, Latov N.

Department of Neurology and Neuroscience, Weill Medical College of Cornell

University, 525 E. 68th St, New York City, NY 10021, United States.

Gene expression analysis previously identified molecular markers that are

up-regulated in sural nerve biopsies from patients with chronic inflammatory

demyelinating polyneuropathy (CIDP). To determine whether the same or additional

genes are also up-regulated in skin, we applied gene microarray profiling and

quantitative real-time PCR (qPCR) analysis to skin punch biopsies from patients

with CIDP and controls.

Five genes, allograft inflammatory factor 1 (AIF-1), lymphatic hyaluronan

receptor (LYVE-1/XLKD1), FYN binding protein (FYB), P2RY1 (purinergic receptor

P2Y, G-protein-coupled, 1), and MLLT3 (myeloid/lymphoid or mixed-lineage

leukemia translocated to, 3), all associated with immune cells or inflammatory

processes, were elevated in punch skin biopsies from patients with CIDP as

compared to normal subjects or patients with Charcot-Marie-Tooth Type 1 (CMT1).

The average fold change of the 5 genes over normal expression, as determined by

qPCR, was significantly elevated in skin biopsies from patients with CIDP in

comparison to CMT1 or diabetic neuropathy, and similar to that seen in Lyme

disease. The findings indicate the presence of inflammatory changes in the skin

of patients with CIDP.