stachy, lungs,1998

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effects of stachy,condida and toxin on lung surfactant 1998

1998;6(1):27-33.

Effects of Stachybotrys chartarum (atra) conidia and isolated toxin on lung

surfactant production and homeostasis.

Mason CD, Rand TG, Oulton M, Mac JM, JE.

Department of Biology, St. 's University, Halifax, Nova Scotia, Canada.

This study evaluated the effects of Stachybotrys chartarum conidia and a

trichothecene, isosatratoxin-F, on choline incorporation into DSPC by fetal

rabbit alveolar type II cells and on alveolar surfactant subtypes in mice.

Exposure of fetal rabbit type II cells to S. chartarum conidia at concentrations

of 10(3) to 10(6) conidia ml(-1) significantly depressed [3H] choline

incorporation after 24 h of exposure. Exposure of the rabbit cells to 10(5) to

10(6) conidia ml(-1) also resulted in significantly depressed [3H] choline

uptake after 48 h. Additionally, fetal rabbit alveolar type II cells exposed to

isosatratoxin-F in concentrations ranging from 10(-9) to 10(-4) M showed a

significant reduction in [3H] choline incorporation into DSPC. Alveolar

surfactant phospholipid concentrations in the different metabolic subfractions

of lung lavage fluid of mice intratracheally exposed to either 50 microl of

10(7) ml(-1) S. chartarum conidia or 50 microl 10(-7) M isosatratoxin-F showed

some significant changes at 12, 24, 48, and 72 h post-exposure, compared to the

surfactant subfractions of control mice which were either untreated, exposed to

saline or to 50 microl of 10(-7) ml(-1) Cladosporium cladosporioides conidia. In

both the S. chartarum- and the isosatratoxin-F-treated mice, exposure

significantly increased P10, P100, and S100 phospholipid concentrations, while

the P60 phospholipid concentrations were depressed. In contrast, C.

cladosporioides-treated mice showed only one significant change in subfraction

phospholipid concentration: P60 was depressed at 48 h post-exposure. These

results reveal that alveolar type II cells are sensitive to exposure to S.

chartarum conidia and to isosatratoxin F. Sensitivity is manifest by alterations

in the normal metabolic processing of alveolar surfactant. In exposed mice, this

effect appears to involve a significant increase in newly secreted surfactant

and an accumulation of the used surfactant forms.

PMID: 9851509 [PubMed - indexed for MEDLINE]

reference fromsmoking,lungs

http://www.tobaccoinduceddiseases.com/content/2/1/3