Research & Technical Areas: Chemical/Bio Defense

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SITIS Topic Details

Proposals Accepted:

Program: STTR

Topic Number: A10a-T021 (Army)

Title: DIPAIN based assay for the T-2 Toxin

Research & Technical Areas: Chemical/Bio Defense

http://www.dodsbir.net/Sitis/display_topic.asp

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Acquisition Program:

Objective: Develop a rapid assay for T-2 Toxin.

Description: The T-2 toxin belongs to the family of trichothecene mycotoxins

that are produced by fungi. The T-2 toxin was first found in stored corn in

1971. Trichothecene mycotoxins are extremely hazardous to humans. Exposure to

nanogram quantities of T-2 toxin can cause severe reactions. Central nervous

systems injury as well as gastrointestinal and hematological toxicity can occur

from ingestion or inhalation of very small quantities of these toxins. A very

promising approach for the detection of trace quantities of trichothecene

mycotoxins involves the use of 2-(diphenylacetyl)-l,3-indanedione-l-hydrazone

(DIPAIN II) and its derivatives as reagents on solid supports. DIPAIN

derivatives undergo significant fluorescence enhancement in the visible range

under UV excitation when in contact with trichothecene mycotoxins. The presence

of the toxin is indicated by the enhanced fluorescence of the DIPAIN derivative.

The goal of this program is to develop DIPAIN-derivative based test-strips that

indicate the presence of trace quantities of trichothecene mycotoxins in aqueous

solutions. The T-2 toxin will be used as a test case for this effort.

PHASE I: Examine the interaction of T-2 toxin

(12,13-epoxytrichothec-9-ene-3,4,8,15-tetraol 4,15-diacetate

8-(3-methylbutanoate)) and DIPAIN-II

(2-(diphenylacetyl)-l,3-indanedione-l-hydrazone). Design an assay in the form of

a test strip that can detect 50 nanograms of T-2 toxin in less than 10 seconds

using the enhanced fluorescence of the DIPAIN-II molecule or other suitable

DIPAIN derivatives. Examine UV sources that are suitable for field usage that

can be used to detect the enhanced fluorescence of the DIPAIN-II molecule.

PHASE II: Produce prototype test strips for detecting the T-2 Toxin using

DIPAIN-II or other suitable DIPAIN derivatives. Design and build a hand-held

ticket reader for detecting the ticket reader should battery operated and weigh

less than 5 pounds. Demonstrate detection of 50 nanograms for less of the T-2

toxin on the test strip in 10 seconds or less. Examine methods for extending

this technology to other trichothecene mycotoxins. In particular examine methods

where multiple toxins can be monitored on a single test strip in a multiplexed

mode.

PHASE III DUAL USE APPLICATIONS: There are environmental applications for a

rapid, inexpensive method to monitor for trichothecene mycotoxins in food and

water. Food safety is essential to citizens of the US. Also, first responders

such as Civilian Support Teams and Fire Departments have a critical need for a

rugged, inexpensive sensor that can be transported to the field to test for

possible contamination by toxins.

References:

1. A. Mosher and A. Brawn,

2-aralkanoyl-1-3-indanedione,1-mono-hydrozones, U.S. Patent Number 2,883,423,

1959.

2. Thaddeus J. Novak and Quinn-Doggett,

" 2-(Diphenylacetyl)-1,3-Indanedione-1-Hydrazone (Dipain) Derivatives for

Detection of Trichothecene Mycotoxins " , Analytical Letters, Volume 24, Issue 6,

pages 913-924, June 1991.

3. P. Ashman, H. , and J. Poziomek, " Decision Tree for

chemical detection applicatons, " Anal. Chem., Vol. 57, pages 1951-1955, 1985.

4. P.N. Krishnan, Luke A. Burke, and O. Jensen, " Theoretical study of the

two tautomers of 2-(diphenylacetyl)-1,3-indandione

1-(p-(dimethylamino)benzaldazine) " , Journal of Molecular Structure (THEOCHEM),

volume 305, pages 9-12, 1994.

5. J. Poziomek and Eleanor V. Crabtree, " Chemical Detection of Selected

Hallucinogens in Water " , Analytical Letters, Volume 14, Issue 14, pages

1185-1189, 1981.

6. Fun S. Chu, " Mycotoxin Analysis " , in Analyzing Food for Nutrition Labeling

and Hazardous Contaminants, Ike J. Jeon and G. Ikins, editors, Series:

Food Science and Technology Volume: 65, CRC Press, pages 283-332, 1994.

Keywords: trichothecene mycotoxins, DIPAIN, T-2 Toxin, UV spectrum.

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