Monoclonal Antibodies Farmer’s Lung Antigens Specific Binding IgG

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Here is another one from 1993:

These purified antigens can be used as reliable reagents in the specific

diagnosis of farmer’s lung disease.

_Vol. 102, No. 1, 1993 _

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Original Paper

Monoclonal Antibodies against Farmer’s Lung Antigens having Specific

Binding to IgG Antibodies

Anoopa Kumar, Elms, Viswanath P. Kurup

Department of Medicine, Allergy-Immunology Division, Medical College of

Wisconsin, V.A. Research Service 151, Milwaukee, Wisc., USA

(http://content.karger.com/ProdukteDB/produkte.asp?Doi=236552#AC) Int Arch

Allergy Immunol 1993;102:67-71 (DOI: 10.1159/000236552)

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Words

* Monoclonal antibodies

* Farmer’s lung disease

* IgG antibodies

* Thermophilic actinomycetes

* <ITALIC>Saccharopolyspora rect

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Abstract

Hypersensitivity pneumonitis resulting from environmental exposure to

Saccharopolyspora rectivirgula (Micropolyspora faeni) among farmers has been

well recognized. The diagnosis of the disease depends on demonstration of

circulating antibodies against S. rectivirgula. However, dependable pure

antigens are not available for serodiagnosis. In the present study we have

employed hybridoma technology to obtain monoclonal antibodies against S.

rectivirgula antigens. These monoclonal antibodies were employed to purify

antigens through affinity chromatography. When tested in ELISA, high levels of

antibodies were demonstrated against these antigens in farmer’s lung

patient sera compared to exposed but asymptomatic individuals from the same

household. In Western blots patient sera reacted with components of crude

antigens with molecular masses of 28, 35, 60, 65 and 68 kD and 4 components

above 100 kD, while the monoclonal antibodies reacted only with the 60-kD

protein. These purified antigens can be used as reliable reagents in the

specific diagnosis of farmer’s lung disease.

Copyright © 1993 S. Karger AG, Basel

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Contacts

Correspondence to: Dr. Viswanath P. Kurup, Allergy-Immunology Division,

Research Service 151, V.A. Medical Center, Milwaukee, WI 53295–1000 (USA)

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Information

Published online: September 03, 2009

Number of Print Pages : 5

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