RE: Viable Bacteria in spaces; was Re: infra red

[Guest guest]

I am out of the office until the middle of next week. I would have to look at

the data table. We cultuted on sheep blood agar. I do not recall off the top

the culture media for gram positive.

Jack Thrasher, Ph.D.

Toxicologist, Immunotoxicologist, Fetal toxicologist

Cell - 575-937-1150 Cell-Thrasher

Crawley, M.ED., LADC

Trauma Specialist

Cell -775-309-3994

www.drthrasher.org

From: scottarmour@...

Date: Thu, 23 Dec 2010 15:48:53 +0000

Subject: [] Viable Bacteria in spaces; was Re: infra red

I'm curious, what/how are you testing for the bacteria? When you report

2.5million/g, is that based on the cultures? or is that a relative measure

extrapolated from the endotoxin sampling?

I've rarely seen much live/viable (in cfu's) once the surfaces and components

being tested are dry. The bacteria doesn't live without the water. So several

weeks after cleanup is unlikely to find viable bacteria in the samples.

Endotoxins are of course a different matter with regard to remaining in the

environment.

I realize that in the last few years we have seen research that is indicating

live/viable/culturable bacteria after weeks, even months. I'm skeptical. Maybe

spores are surviving. But some of the species don't produce spores. I admit,

I've not studied in detail the reports and the results.

> >

>

> > A negative IR may mean the water which was once there is now gone. But any

mold which grew while damp will still be there undetected.

> >

> > Carl Grimes

> > Healthy Habitats LLC

> > (fm my Blackberry)

> >

> >

>

>

>

>

>

>

[Guest guest]

: The results were in CFU. The apartment had water intrusion of rain and

sewage overflow. We took samples of what appeared to be mold growth in bulk.

The apartment was still damp. We also found increased levels of Gram positive

bacteria. When I return to my office next week I can give more details after i

review the file on this client. Of great interest is that the three occupants

have skeletal muscle myopathy for which mitochondrial studies are being done.

They were all positive for urine and nasal trichothecenes, aflatoxins and

ochratoxin. Aflatoxins are a mitochondrial poison. I am still searching the

literature on trichothecenes and ochratoxin regarding mitochondrial function.

Jack Thrasher, Ph.D.

Toxicologist, Immunotoxicologist, Fetal toxicologist

Cell - 575-937-1150 Cell-Thrasher

Crawley, M.ED., LADC

Trauma Specialist

Cell -775-309-3994

www.drthrasher.org

From: scottarmour@...

Date: Thu, 23 Dec 2010 15:48:53 +0000

Subject: [] Viable Bacteria in spaces; was Re: infra red

I'm curious, what/how are you testing for the bacteria? When you report

2.5million/g, is that based on the cultures? or is that a relative measure

extrapolated from the endotoxin sampling?

I've rarely seen much live/viable (in cfu's) once the surfaces and components

being tested are dry. The bacteria doesn't live without the water. So several

weeks after cleanup is unlikely to find viable bacteria in the samples.

Endotoxins are of course a different matter with regard to remaining in the

environment.

I realize that in the last few years we have seen research that is indicating

live/viable/culturable bacteria after weeks, even months. I'm skeptical. Maybe

spores are surviving. But some of the species don't produce spores. I admit,

I've not studied in detail the reports and the results.