Guest guest Posted January 10, 2011 Report Share Posted January 10, 2011 This is very good and yet troubling to me.I had always thought that the mold spores collected on the plate within my home (aspergillus genera, penicillium genera, etc.) at about 700 per cu meters was explainable by the colonies of mold growing in the basement. But I didn't really think that through. The spores would have had to woosh up into my second floor unit at that moment and then immediately settle out of the air. Right? Because how could I have so many spores without a constant colony supply and how could it be so far away? Unless the carpet in the hallway is actively growing mold--it has over 200,000 spores per cu meters but the back door is always closed on that hallway. Isn't it more likely that there is a hidden source of mold within my unit itself that is causing the release of those spores? The other thing I wonder is my continued, daily allergic reaction. The mold was cleaned up in the basement. Water has come in again but I do not see active colonies yet. Plus, the air is quite dry, under 40%. How could I--and my cat, who has had three bouts of allergic bronchitis since moving in--continue to have the same allergic symptoms? Please help! Thanks, AN Sent from my iPhone On Jan 10, 2011, at 1:09 PM, " Carl E. Grimes " <grimes@...> wrote: I think it will help if we all have a clearer understanding of what mold is and what it isn't. Google has some really good photos which can be accessed by searching for " mold growth " and then clicking on " Images. " What you see is often like the first image (and most of the others). This iconic image by Terry Brennan shows colonies of a variety of types of mold resulting from airborne moisture flow through the top of the door and across the ceiling. It is not growing on the walls. Where you sample will make a huge difference in the lab results. The image 3rd to the right is much different. Mold is growing on the walls but not the ceiling because the water source soaked up the drywall from the floor. Also, notice how the color changes right near the top of the growth. Different mold because of slightly different moisture levels at the edge of the dampness. If this area were to be sampled you would get drastically different results based on the exact location of the sample. If any of the above mold growth were sporulating then their spores, along with others having nothing to do with the situation, might show up depending on the type of sample, where collected, etc etc. BTW, I've seen air samples from rooms like many in these images with the conclusion that " there is no mold problem. " Because of the assinine and discredited methods of interpreting results, plus the bias of who collected the samples. But that is for another time. Scrolling down you will see " Page 2 " and the first image is a culture plate with mold growth. It looks much different than the previous images for obvious reasons and because it is from a settling plate. Only the spores in the air which are alive and which are heavy enough to settle from the air and happen by chance to land on the plate which has the right kind of food (agar) will grow. They may or may not be from the " growth " in the previous images. What none of these show is bacteria, actinomycetes, and anything other then mold *growth.* The growth, or colony, is what is visible. Spores are the seeds and are too small to see with the naked eye. Got to Page 4 and the first image shows spores flowing from a single stalk of the mold growth colony. All of which is too small to see with the naked eye. Page 5, second down, left side. This identifies some of the many parts of a mold growth colony. The illustration below it is a diagram of mold growth. BTW, the MC (moisture content of the surface) will determine whether or not mold spores will germinate and form colonies. Of all the different types of spores always in the air only some will grow. Change the MC slightly and different ones will grow. BTW, the surface with MC where the mold is growing is where the " biofilm " will form. It is not automatic and is not part of the mold " plant. " It is a film of dampness along with dust, old mold, bacteria and other " filth " which accumulates on the surface over time. As it develops other molds and bacteria previously unable to grow may now start to " make babies " and further populate their little " city " called a " biofilm. " Go back to the culture plate on Page 2. What you see is mold growth which will have some spores. But the massive amount of substance is " biomass " and not spores. Which is part of what Dr Thrasher means when he says about mold, " It's more than just spores. " But it means much more. With dampness there will also be growth of bacteria. It just doesn't grow in a way we can see it and few test for it. General samples for bacteria is limited to the shape (rod, cone, spiral) and whether it changes color with a stain (gram positive) or doesn't (gram negative). Carl Grimes Healthy Habitats LLC Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 10, 2011 Report Share Posted January 10, 2011 AN, Your questions require some explanation about spores, sporulation, air pathways, and sampling. When spores are released they usually become airborne like dandelion fluff. (Except recent evidence says some shoot out like a bullet at a distance of over a foot!) Because they are so small they can stay airborne like dust. The heavier ones settle quickly but the lighter ones can take hours. Fragments are even smaller and can sometimes stay in the air all the time. They will travel to where ever the air goes. There will always be some slight air movement between floors of a building because nothing is built airtight. So even if it takes a day for the basement air to slowly circulate to the 2nd level that's plenty of time if the mold growth has been there for at least a day. Even if the mold growth is removed properly, spores and fragments will continue to be in the air and on surfaces where they settled like dust. If on surfaces then when the air is disturbed by walking by or sitting down they become airborne again and available to be breathed into your nose, sinus, and lungs. If you have forced air heat or A/C then the fan forcibly and quickly circulates air from all places in the house to all other places fairly uniformly. So even if you don't go into your basement the air in your basement comes to you. Again, it's not just the spores but also hyphae and various fragments. So a settling plate could show zero (0) spores even when there is massive mold growth somewhere in the house. Also, and especially with forced air, the heat tends to dry the spores rendering them unable to germinate in dampness or on the settling plate. Next you asked about at hidden source of mold somewhere else in you house. Exactly! There might be and there might not be. And this sample gives no information about whether there is or isn't. It's impossible to do determine that by air samples alone. Other information such as moisture levels and history is needed to sort that out. Finally, you and your cats could well be reacting to the airborne mold and fragments discussed above; or to the bacteria which was growing along with the mold; or to chemicals used in the removal of the mold (if any were used); or something else. Once sensitized by the mold in the basement your body is still sensitive to other irritants and allergens. There may be no mold left to react to but other things are still triggering the inflammatory response. If someone hits you in the face does the pain stop when they remove their fist? Would not your pain increase from even a loving caress? Or from actions totally unrelated to the hit, such as walking or chewing? But at this point we don't have answers to those questions. Carl Grimes Healthy Habitats LLC ----- This is very good and yet troubling to me.I had always thought that the mold spores collected on the plate within my home (aspergillus genera, penicillium genera, etc.) at about 700 per cu meters was explainable by the colonies of mold growing in the basement. But I didn't really think that through. The spores would have had to woosh up into my second floor unit at that moment and then immediately settle out of the air. Right? Because how could I have so many spores without a constant colony supply and how could it be so far away? Unless the carpet in the hallway is actively growing mold--it has over 200,000 spores per cu meters but the back door is always closed on that hallway. Isn't it more likely that there is a hidden source of mold within my unit itself that is causing the release of those spores? The other thing I wonder is my continued, daily allergic reaction. The mold was cleaned up in the basement. Water has come in again but I do not see active colonies yet. Plus, the air is quite dry, under 40%. How could I--and my cat, who has had three bouts of allergic bronchitis since moving in--continue to have the same allergic symptoms? Please help! Thanks, AN Sent from my iPhone On Jan 10, 2011, at 1:09 PM, " Carl E. Grimes " <grimes@...> wrote: I think it will help if we all have a clearer understanding of what mold is and what it isn't. Google has some really good photos which can be accessed by searching for " mold growth " and then clicking on " Images. " What you see is often like the first image (and most of the others). This iconic image by Terry Brennan shows colonies of a variety of types of mold resulting from airborne moisture flow through the top of the door and across the ceiling. It is not growing on the walls. Where you sample will make a huge difference in the lab results. The image 3rd to the right is much different. Mold is growing on the walls but not the ceiling because the water source soaked up the drywall from the floor. Also, notice how the color changes right near the top of the growth. Different mold because of slightly different moisture levels at the edge of the dampness. If this area were to be sampled you would get drastically different results based on the exact location of the sample. If any of the above mold growth were sporulating then their spores, along with others having nothing to do with the situation, might show up depending on the type of sample, where collected, etc etc. BTW, I've seen air samples from rooms like many in these images with the conclusion that " there is no mold problem. " Because of the assinine and discredited methods of interpreting results, plus the bias of who collected the samples. But that is for another time. Scrolling down you will see " Page 2 " and the first image is a culture plate with mold growth. It looks much different than the previous images for obvious reasons and because it is from a settling plate. Only the spores in the air which are alive and which are heavy enough to settle from the air and happen by chance to land on the plate which has the right kind of food (agar) will grow. They may or may not be from the " growth " in the previous images. What none of these show is bacteria, actinomycetes, and anything other then mold *growth.* The growth, or colony, is what is visible. Spores are the seeds and are too small to see with the naked eye. Got to Page 4 and the first image shows spores flowing from a single stalk of the mold growth colony. All of which is too small to see with the naked eye. Page 5, second down, left side. This identifies some of the many parts of a mold growth colony. The illustration below it is a diagram of mold growth. BTW, the MC (moisture content of the surface) will determine whether or not mold spores will germinate and form colonies. Of all the different types of spores always in the air only some will grow. Change the MC slightly and different ones will grow. BTW, the surface with MC where the mold is growing is where the " biofilm " will form. It is not automatic and is not part of the mold " plant. " It is a film of dampness along with dust, old mold, bacteria and other " filth " which accumulates on the surface over time. As it develops other molds and bacteria previously unable to grow may now start to " make babies " and further populate their little " city " called a " biofilm. " Go back to the culture plate on Page 2. What you see is mold growth which will have some spores. But the massive amount of substance is " biomass " and not spores. Which is part of what Dr Thrasher means when he says about mold, " It's more than just spores. " But it means much more. With dampness there will also be growth of bacteria. It just doesn't grow in a way we can see it and few test for it. General samples for bacteria is limited to the shape (rod, cone, spiral) and whether it changes color with a stain (gram positive) or doesn't (gram negative). Carl Grimes Healthy Habitats LLC Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 10, 2011 Report Share Posted January 10, 2011 Hi Carl, Thanks--I love the metaphors! Dandelion fluff, bullets...would be so cool if I didn't hate mold with a burning passion (mold is neutral but my feelings are not). Anyway, I know that some spores are heavier than others and settle out quickly--so what I was thinking was, wouldn't the air need a constant resupply of those spores (or hyphae)? We don't have central air and do not use our condo's back door and do not go in the basement. So, though I'd been reading about convection air flow from, say, opening windows, I still thought that maybe a more likely source would be in the condo. I am definitely, definitely sensitized now to the extreme. Perfume used to be a mild irritant and now it sends me into fits of coughing and sneezing. I've never been able to handle third hand smoke. But nobody smokes in our building (isn't allowed) and of us four owners, no one uses heavy scents. Of course I use scent free products. So I'm pretty sure it's the mold or associated bacteria--though it's complicated, I know. I feel that I am now inflamed by the world--I rarely get relief these days, not for more than an hour, from itching, sneezing, stinging eyes, etc. How does bacteria " travel " ? Can bacteria in a basement affect occupants two stories up? I know some bacteria are dispersed through the air, some through contact. Can bacteria go through fabric? (Now I'm thinking about the recent discussion about vaginosis)? How does bacteria affect people aside from tissue colonization? Thanks, Carl! I really love some of your ways of looking at things--dandelions...that puts a smile on a very ugly (to me) topic--blech, mold, blech WDBs! AN (PS still planning on calling re:book but am still working on this other manuscript) On Jan 10, 2011, at 7:42 PM, " Carl E. Grimes " <grimes@...> wrote: AN, Your questions require some explanation about spores, sporulation, air pathways, and sampling. When spores are released they usually become airborne like dandelion fluff. (Except recent evidence says some shoot out like a bullet at a distance of over a foot!) Because they are so small they can stay airborne like dust. The heavier ones settle quickly but the lighter ones can take hours. Fragments are even smaller and can sometimes stay in the air all the time. They will travel to where ever the air goes. There will always be some slight air movement between floors of a building because nothing is built airtight. So even if it takes a day for the basement air to slowly circulate to the 2nd level that's plenty of time if the mold growth has been there for at least a day. Even if the mold growth is removed properly, spores and fragments will continue to be in the air and on surfaces where they settled like dust. If on surfaces then when the air is disturbed by walking by or sitting down they become airborne again and available to be breathed into your nose, sinus, and lungs. If you have forced air heat or A/C then the fan forcibly and quickly circulates air from all places in the house to all other places fairly uniformly. So even if you don't go into your basement the air in your basement comes to you. Again, it's not just the spores but also hyphae and various fragments. So a settling plate could show zero (0) spores even when there is massive mold growth somewhere in the house. Also, and especially with forced air, the heat tends to dry the spores rendering them unable to germinate in dampness or on the settling plate. Next you asked about at hidden source of mold somewhere else in you house. Exactly! There might be and there might not be. And this sample gives no information about whether there is or isn't. It's impossible to do determine that by air samples alone. Other information such as moisture levels and history is needed to sort that out. Finally, you and your cats could well be reacting to the airborne mold and fragments discussed above; or to the bacteria which was growing along with the mold; or to chemicals used in the removal of the mold (if any were used); or something else. Once sensitized by the mold in the basement your body is still sensitive to other irritants and allergens. There may be no mold left to react to but other things are still triggering the inflammatory response. If someone hits you in the face does the pain stop when they remove their fist? Would not your pain increase from even a loving caress? Or from actions totally unrelated to the hit, such as walking or chewing? But at this point we don't have answers to those questions. Carl Grimes Healthy Habitats LLC Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 AN, I'll try to keep this short about bacteria but we have to sort out some concepts and definitions too complex for here. Simply put, bacteria have a physical size although they are too small to see. They are bigger than air molecules but smaller than dust. Because they are alive they eat and excrete waste. The waste is molecules. If the " holes " in fabric are smaller than the bacteria then the bacteria usually can't move through it. But reproductive buds might. If the bacteria, or especially mold, chemically breaksdown the fabric then the " hole " is bigger and perhaps they can. So bacteria can become airborne and travel on normal air currents. Their " waste " products can also. But because that is chemical in nature they can react chemically with a surface they are on rather than inertly like a piece of sand or dust. Inside the body is very different than outside the body. When bacteria infect they may or may not be able to penetrate or move through membranes of organs, blood vessels, lungs, etc. In trying to keep this short and simple I may be just creating confusion and more questions. These types of questions are excellent and need to be asked. But a useful answer depends on understanding micro-organisms in general plus particles and their size, their aerodynamic properties which determine how they travel thru air, moisture content vs water activity, and a whole cirriculum of other topics. It is often simpler to accept what Joe Lstiburek calls the 4 Ps. People, Pollutants, Pathways, and Pressure. Without people there is no people problem. Pollutants are whatever is not supportive or is harmful to people. Pathways is how the pollutants move from where they are generated and growing to where the person is. Pressure, usually air pressure but also vapor pressure and a few others, is what moves the pollutants through the pathway to where the people are. Carl Grimes Healthy Habitats LLC ----- Hi Carl, Thanks--I love the metaphors! Dandelion fluff, bullets...would be so cool if I didn't hate mold with a burning passion (mold is neutral but my feelings are not). Anyway, I know that some spores are heavier than others and settle out quickly--so what I was thinking was, wouldn't the air need a constant resupply of those spores (or hyphae)? We don't have central air and do not use our condo's back door and do not go in the basement. So, though I'd been reading about convection air flow from, say, opening windows, I still thought that maybe a more likely source would be in the condo. I am definitely, definitely sensitized now to the extreme. Perfume used to be a mild irritant and now it sends me into fits of coughing and sneezing. I've never been able to handle third hand smoke. But nobody smokes in our building (isn't allowed) and of us four owners, no one uses heavy scents. Of course I use scent free products. So I'm pretty sure it's the mold or associated bacteria--though it's complicated, I know. I feel that I am now inflamed by the world--I rarely get relief these days, not for more than an hour, from itching, sneezing, stinging eyes, etc. How does bacteria " travel " ? Can bacteria in a basement affect occupants two stories up? I know some bacteria are dispersed through the air, some through contact. Can bacteria go through fabric? (Now I'm thinking about the recent discussion about vaginosis)? How does bacteria affect people aside from tissue colonization? Thanks, Carl! I really love some of your ways of looking at things-- dandelions...that puts a smile on a very ugly (to me) topic--blech, mold, blech WDBs! AN (PS still planning on calling re:book but am still working on this other manuscript) On Jan 10, 2011, at 7:42 PM, " Carl E. Grimes " <grimes@...> wrote: AN, Your questions require some explanation about spores, sporulation, air pathways, and sampling. When spores are released they usually become airborne like dandelion fluff. (Except recent evidence says some shoot out like a bullet at a distance of over a foot!) Because they are so small they can stay airborne like dust. The heavier ones settle quickly but the lighter ones can take hours. Fragments are even smaller and can sometimes stay in the air all the time. They will travel to where ever the air goes. There will always be some slight air movement between floors of a building because nothing is built airtight. So even if it takes a day for the basement air to slowly circulate to the 2nd level that's plenty of time if the mold growth has been there for at least a day. Even if the mold growth is removed properly, spores and fragments will continue to be in the air and on surfaces where they settled like dust. If on surfaces then when the air is disturbed by walking by or sitting down they become airborne again and available to be breathed into your nose, sinus, and lungs. If you have forced air heat or A/C then the fan forcibly and quickly circulates air from all places in the house to all other places fairly uniformly. So even if you don't go into your basement the air in your basement comes to you. Again, it's not just the spores but also hyphae and various fragments. So a settling plate could show zero (0) spores even when there is massive mold growth somewhere in the house. Also, and especially with forced air, the heat tends to dry the spores rendering them unable to germinate in dampness or on the settling plate. Next you asked about at hidden source of mold somewhere else in you house. Exactly! There might be and there might not be. And this sample gives no information about whether there is or isn't. It's impossible to do determine that by air samples alone. Other information such as moisture levels and history is needed to sort that out. Finally, you and your cats could well be reacting to the airborne mold and fragments discussed above; or to the bacteria which was growing along with the mold; or to chemicals used in the removal of the mold (if any were used); or something else. Once sensitized by the mold in the basement your body is still sensitive to other irritants and allergens. There may be no mold left to react to but other things are still triggering the inflammatory response. If someone hits you in the face does the pain stop when they remove their fist? Would not your pain increase from even a loving caress? Or from actions totally unrelated to the hit, such as walking or chewing? But at this point we don't have answers to those questions. Carl Grimes Healthy Habitats LLC ---------- The following section of this message contains a file attachment prepared for transmission using the Internet MIME message format. If you are using Pegasus Mail, or any other MIME-compliant system, you should be able to save it or view it from within your mailer. If you cannot, please ask your system administrator for assistance. ---- File information ----------- File: DEFAULT.BMP Date: 15 Jun 2009, 23:10 Size: 358 bytes. Type: Unknown Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 Hi Carl, and thank you. This gives me great, basic information. No confusion. It makes perfect sense. From: Carl E. Grimes <grimes@...> Subject: Re: [] Mold Tutorial from Images Date: Wednesday, January 12, 2011, 8:37 AM AN, I'll try to keep this short about bacteria but we have to sort out some concepts and definitions too complex for here. Simply put, bacteria have a physical size although they are too small to see. They are bigger than air molecules but smaller than dust. Because they are alive they eat and excrete waste. The waste is molecules. If the " holes " in fabric are smaller than the bacteria then the bacteria usually can't move through it. But reproductive buds might. If the bacteria, or especially mold, chemically breaksdown the fabric then the " hole " is bigger and perhaps they can. So bacteria can become airborne and travel on normal air currents. Their " waste " products can also. But because that is chemical in nature they can react chemically with a surface they are on rather than inertly like a piece of sand or dust. Inside the body is very different than outside the body. When bacteria infect they may or may not be able to penetrate or move through membranes of organs, blood vessels, lungs, etc. In trying to keep this short and simple I may be just creating confusion and more questions. These types of questions are excellent and need to be asked. But a useful answer depends on understanding micro-organisms in general plus particles and their size, their aerodynamic properties which determine how they travel thru air, moisture content vs water activity, and a whole cirriculum of other topics. It is often simpler to accept what Joe Lstiburek calls the 4 Ps. People, Pollutants, Pathways, and Pressure. Without people there is no people problem. Pollutants are whatever is not supportive or is harmful to people. Pathways is how the pollutants move from where they are generated and growing to where the person is. Pressure, usually air pressure but also vapor pressure and a few others, is what moves the pollutants through the pathway to where the people are. Carl Grimes Healthy Habitats LLC Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 Carl and : We are losing sight of key elements. There is sufficient information in the literature (Reponen's group, Brasel, Gorny, etc). Activity in the indoor environment from humans causes the production of energy waves that are sufficient to fractionate the colonies of fungi and bacteria. This causes the releases of particulates into three size ranges: <1 micron; 1-2.5 microns and >2.5 microns. The <1 micron fractions is up to 500 times more concentrated than are the larger particles. The finer particulates are cell wall fragments of both fungi and bacteria. They are inhaled deep into the alveoli and do the following: 1) release their toxins into the blood stream and 2) nano size particle actually cross the alveolar membrane and enter the systemic circulation. Once again, emphasizes on spore counts by various members of this forum is towards spore counts. We need to turn our attention towards particulates of all sizes. The finer particulates are components of the dust that infiltrates all nooks and crannies. The particulates can also bind to fabrics (clothes, couches. carpets, beddings). I have found that the fine particles contain the mycotoxins, endotoxins, beta glucans, among other contaminants. Finally, there is sufficient information in the literature that shows inhaled nano particles are distributed throughout the body, including the brain in humans and animals. I refer all to the research of Dr. Lilian Garciduenas regarding the systemic distribution in children, young adults and canines. Re: [] Mold Tutorial from Images AN, I'll try to keep this short about bacteria but we have to sort out some concepts and definitions too complex for here. Simply put, bacteria have a physical size although they are too small to see. They are bigger than air molecules but smaller than dust. Because they are alive they eat and excrete waste. The waste is molecules. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 Excellent points, Dr Thrasher. I'd like to add two points and a resource: 1. As the particle size decreases the surface area increases, which results in an increase in the activity of chemical properties in addition to the particulate properties. Adding further complexity to inteaction with materials and inside the body. 2. As the particle size approaches nano there is an increased accumulation of the particles at the branching junctions of the alveoli resulting in a larger quantity of the total than with each one individually. FYI, nano needs to be defined because there is a lot of confusion and fear because it is so foreign sounding and unknown. There are various definitions but the more general one is the range of nano sized particles begins at aout 0.01 microns and extends smaller. Some say it begins at 0.001 microns. Either way it's extremely small. In context and in ranges by factors of 10 - Human hair is about 100 microns in diameter. - We can see as small as about 10 microns in ideal light (dust in a light shaft in a dark room). - Many irritants and allergens including mold spores are in the 10- 1 micron range. - HEPA filters are rated at 0.3 microns. - Nano begins around 0.01 microns but others say 0.001. - Air molecules, by comparison, are about 0.00001 microns. For those oriented more visually than by numbers, there's an amazing interactive illustration comparing sizes from the head of a pin to dust mites through pollen, bacteria and several viruses. http://www.cellsalive.com/howbig.htm Carl Grimes Healthy Habitats LLC ------ Carl and : We are losing sight of key elements. There is sufficient information in the literature (Reponen's group, Brasel, Gorny, etc). Activity in the indoor environment from humans causes the production of energy waves that are sufficient to fractionate the colonies of fungi and bacteria. This causes the releases of particulates into three size ranges: <1 micron; 1-2.5 microns and >2.5 microns. The <1 micron fractions is up to 500 times more concentrated than are the larger particles. The finer particulates are cell wall fragments of both fungi and bacteria. They are inhaled deep into the alveoli and do the following: 1) release their toxins into the blood stream and 2) nano size particle actually cross the alveolar membrane and enter the systemic circulation. Once again, emphasizes on spore counts by various members of this forum is towards spore counts. We need to turn our attention towards particulates of all sizes. The finer particulates are components of the dust that infiltrates all nooks and crannies. The particulates can also bind to fabrics (clothes, couches. carpets, beddings). I have found that the fine particles contain the mycotoxins, endotoxins, beta glucans, among other contaminants. Finally, there is sufficient information in the literature that shows inhaled nano particles are distributed throughout the body, including the brain in humans and animals. I refer all to the research of Dr. Lilian Garciduenas regarding the systemic distribution in children, young adults and canines. Re: [] Mold Tutorial from Images AN, I'll try to keep this short about bacteria but we have to sort out some concepts and definitions too complex for here. Simply put, bacteria have a physical size although they are too small to see. They are bigger than air molecules but smaller than dust. Because they are alive they eat and excrete waste. The waste is molecules. ---------- The following section of this message contains a file attachment prepared for transmission using the Internet MIME message format. If you are using Pegasus Mail, or any other MIME-compliant system, you should be able to save it or view it from within your mailer. If you cannot, please ask your system administrator for assistance. ---- File information ----------- File: DEFAULT.BMP Date: 15 Jun 2009, 23:10 Size: 358 bytes. Type: Unknown Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 Carl: Thank you. I believe our goal is to make sure that the people on this forum understand the complexity. For example, I was recently involved in a school regarding health problems. The schools CIH did air samples only and found no problems. I went in and collected various dust samples. PCR DNA revealed the historical presence of several species of toxic Aspergillus and Penicillium, Stachybotrys and Trichoderma viride among others. We are analyzing the dust for mycotoxins, endotoxins and glucans. The school officials are very chagrin. I cannot wait until they cry foul. I am now recommending additional investigation into hidden sources of the fungi as well as bacteria. All must be aware that school boards are self-insured for the most part. Re: [] Mold Tutorial from Images AN, I'll try to keep this short about bacteria but we have to sort out some concepts and definitions too complex for here. Simply put, bacteria have a physical size although they are too small to see. They are bigger than air molecules but smaller than dust. Because they are alive they eat and excrete waste. The waste is molecules. ---------- The following section of this message contains a file attachment prepared for transmission using the Internet MIME message format. If you are using Pegasus Mail, or any other MIME-compliant system, you should be able to save it or view it from within your mailer. If you cannot, please ask your system administrator for assistance. ---- File information ----------- File: DEFAULT.BMP Date: 15 Jun 2009, 23:10 Size: 358 bytes. Type: Unknown Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 I dont know what to think about useing nanoparticles as a use of drug delivery, I've read articles too that show they get into and stay in several parts of the body. those articles really opened my eyes about how tiny tiny particles get around in the body, mycotoxins are also sometimes refered to as nanoparticles. --- In , " Jack Thrasher, Ph.D. " <toxicologist1@...> wrote: > > Carl and : We are losing sight of key elements. There is sufficient information in the literature (Reponen's group, Brasel, Gorny, etc). Activity in the indoor environment from humans causes the production of energy waves that are sufficient to fractionate the colonies of fungi and bacteria. This causes the releases of particulates into three size ranges: <1 micron; 1-2.5 microns and >2.5 microns. The <1 micron fractions is up to 500 times more concentrated than are the larger particles. The finer particulates are cell wall fragments of both fungi and bacteria. They are inhaled deep into the alveoli and do the following: 1) release their toxins into the blood stream and 2) nano size particle actually cross the alveolar membrane and enter the systemic circulation. > > Once again, emphasizes on spore counts by various members of this forum is towards spore counts. We need to turn our attention towards particulates of all sizes. The finer particulates are components of the dust that infiltrates all nooks and crannies. The particulates can also bind to fabrics (clothes, couches. carpets, beddings). I have found that the fine particles contain the mycotoxins, endotoxins, beta glucans, among other contaminants. Finally, there is sufficient information in the literature that shows inhaled nano particles are distributed throughout the body, including the brain in humans and animals. I refer all to the research of Dr. Lilian Garciduenas regarding the systemic distribution in children, young adults and canines. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 thanks Jack, so it is possable that tiny mold fragments might get in the body and cause infection and maybe even colonization. maybe not probable but possable. --- In , " Jack Thrasher, Ph.D. " <toxicologist1@...> wrote: > > Carl and : We are losing sight of key elements. There is sufficient information in the literature (Reponen's group, Brasel, Gorny, etc). Activity in the indoor environment from humans causes the production of energy waves that are sufficient to fractionate the colonies of fungi and bacteria. This causes the releases of particulates into three size ranges: <1 micron; 1-2.5 microns and >2.5 microns. The <1 micron fractions is up to 500 times more concentrated than are the larger particles. The finer particulates are cell wall fragments of both fungi and bacteria. They are inhaled deep into the alveoli and do the following: 1) release their toxins into the blood stream and 2) nano size particle actually cross the alveolar membrane and enter the systemic circulation. > > Once again, emphasizes on spore counts by various members of this forum is towards spore counts. We need to turn our attention towards particulates of all sizes. The finer particulates are components of the dust that infiltrates all nooks and crannies. The particulates can also bind to fabrics (clothes, couches. carpets, beddings). I have found that the fine particles contain the mycotoxins, endotoxins, beta glucans, among other contaminants. Finally, there is sufficient information in the literature that shows inhaled nano particles are distributed throughout the body, including the brain in humans and animals. I refer all to the research of Dr. Lilian Garciduenas regarding the systemic distribution in children, young adults and canines. > > > Re: [] Mold Tutorial from Images > > AN, > > I'll try to keep this short about bacteria but we have to sort out > some concepts and definitions too complex for here. Simply put, > bacteria have a physical size although they are too small to see. > They are bigger than air molecules but smaller than dust. > Because they are alive they eat and excrete waste. The waste is > molecules. > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 Wow. Okay. Energy waves from simply existing create disturbance? Interesting about the most concentrations at the smallest sizes and the nano particles crossing into the blood. Is your web site complete with this information? Here again, this makes me want more investigation into vaginosis and other " female " issues. I will look up the doctor's work that you mention. I see why bacteria is key to your perspective. It's making a lot of sense now. On Jan 12, 2011, at 12:11 PM, " Jack Thrasher, Ph.D. " <toxicologist1@...> wrote: Carl and : We are losing sight of key elements. There is sufficient information in the literature (Reponen's group, Brasel, Gorny, etc). Activity in the indoor environment from humans causes the production of energy waves that are sufficient to fractionate the colonies of fungi and bacteria. This causes the releases of particulates into three size ranges: <1 micron; 1-2.5 microns and >2.5 microns. The <1 micron fractions is up to 500 times more concentrated than are the larger particles. The finer particulates are cell wall fragments of both fungi and bacteria. They are inhaled deep into the alveoli and do the following: 1) release their toxins into the blood stream and 2) nano size particle actually cross the alveolar membrane and enter the systemic circulation. Once again, emphasizes on spore counts by various members of this forum is towards spore counts. We need to turn our attention towards particulates of all sizes. The finer particulates are components of the dust that infiltrates all nooks and crannies. The particulates can also bind to fabrics (clothes, couches. carpets, beddings). I have found that the fine particles contain the mycotoxins, endotoxins, beta glucans, among other contaminants. Finally, there is sufficient information in the literature that shows inhaled nano particles are distributed throughout the body, including the brain in humans and animals. I refer all to the research of Dr. Lilian Garciduenas regarding the systemic distribution in children, young adults and canines. Re: [] Mold Tutorial from Images AN, I'll try to keep this short about bacteria but we have to sort out some concepts and definitions too complex for here. Simply put, bacteria have a physical size although they are too small to see. They are bigger than air molecules but smaller than dust. Because they are alive they eat and excrete waste. The waste is molecules. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 It is on my web site under complexity of biocontaminants. Re: [] Mold Tutorial from Images Wow. Okay. Energy waves from simply existing create disturbance? Interesting about the most concentrations at the smallest sizes and the nano particles crossing into the blood. Is your web site complete with this information? Here again, this makes me want more investigation into vaginosis and other " female " issues. I will look up the doctor's work that you mention. I see why bacteria is key to your perspective. It's making a lot of sense now. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 Fragments are what is referred to as fragments of dead organisms, not mold or bacterial spores. One needs to have the introduction of larger spores to get colonization or infection. I must point out that the spores of the Actinomycetes are usually one or less micron in size. These can cause an infection as well as the mold spores. You are attempting to read too much into what I wrote. [] Re: Mold Tutorial from Images thanks Jack, so it is possable that tiny mold fragments might get in the body and cause infection and maybe even colonization. maybe not probable but possable. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 Research on animals show that drug laden nanoparrticles may cause organ damage not associated with the target tissues. You can look this up by doing a Google Scholar search [] Re: Mold Tutorial from Images I dont know what to think about useing nanoparticles as a use of drug delivery, I've read articles too that show they get into and stay in several parts of the body. those articles really opened my eyes about how tiny tiny particles get around in the body, mycotoxins are also sometimes refered to as nanoparticles. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 It also becomes obvious how significant the smaller particles are when you see 'dust' in the light in an otherwise dark room like Carl mentioned and how it appears to just be " suspended " in the air, not going anywhere, at least not fast, and realize that those are LARGE particles by contrast to bacteria, viruses and " toxic dust " of the tiniest sizes, and how those must stay suspended in air for very long time ONCE DISTURBED and become airborne. I had people removing carpet and carpet padding for me and I kept asking them to please not tear it up and fling the stuff around but every time I checked on them was the case. After they left I had terrible migraine and so I never had them back and removed the remainder of the carpet myself, w box cutter, cutting squares out and putting them in garbage bag, some every day before garbage pick up. It took me forever but getting rid of things like that can cause a problem if you don't do it carefully...trying to find someone to listen to and understand the danger of flinging dirt inside the home is not easy, and why often professionals will discuss the pros and cons of whether it is worth 'disturbing' an area to remove a contaminant or enclose or encapsulte it if possible...something I didn't understand at one time. > > Wow. Okay. Energy waves from simply existing create disturbance? Interesting about the most concentrations at the smallest sizes and the nano particles crossing into the blood. Is your web site complete with this information? Here again, this makes me want more investigation into vaginosis and other " female " issues. I will look up the doctor's work that you mention. > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted January 12, 2011 Report Share Posted January 12, 2011 ok, that clears that up. thanks. this went back to the question I had asked Carl about mold fragments. posts have been a little confusing lately. --- In , " Jack Thrasher, Ph.D. " <toxicologist1@...> wrote: > > Fragments are what is referred to as fragments of dead organisms, not mold or bacterial spores. One needs to have the introduction of larger spores to get colonization or infection. I must point out that the spores of the Actinomycetes are usually one or less micron in size. These can cause an infection as well as the mold spores. > > You are attempting to read too much into what I wrote. > > Quote Link to comment Share on other sites More sharing options...
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