Hepatitis B vaccine induces apoptotic death in Hepa1-6 cells.

[Guest guest]

http://www.ncbi.nlm.nih.gov/pubmed/22249285/

Apoptosis.

2012 May;17(5):516-27. doi: 10.1007/s10-1.

Hepatitis B vaccine induces apoptotic death in Hepa1-6

cells.

Hamza H,

Cao J,

Li X,

Li C,

Zhu M,

Zhao S.

SourceKey Lab of Agricultural Animal Genetics, Breeding,

and Reproduction of Ministry of Education, College of Animal Science and

Technology, Huazhong Agricultural University, Wuhan, People's Republic of

China. Heyam68_hamza@...

AbstractVaccines can have adverse side-effects, and these

are predominantly associated with the inclusion of chemical additives

such as aluminum hydroxide adjuvant. The objective of this study was to

establish an in vitro model system amenable to mechanistic investigations

of cytotoxicity induced by hepatitis B vaccine, and to investigate the

mechanisms of vaccine-induced cell death. The mouse liver hepatoma cell

line Hepa1-6 was treated with two doses of adjuvanted (aluminium

hydroxide) hepatitis B vaccine (0.5 and 1 g protein per ml) and

cell integrity was measured after 24, 48 and 72 h. Hepatitis B vaccine

exposure increased cell apoptosis as detected by flow cytometry and TUNEL

assay. Vaccine exposure was accompanied by significant increases in the

levels of activated caspase 3, a key effector caspase in the apoptosis

cascade. Early transcriptional events were detected by qRT-PCR. We report

that hepatitis B vaccine exposure resulted in significant upregulation of

the key genes encoding caspase 7, caspase 9, Inhibitor caspase-activated

DNase (ICAD), Rho-associated coiled-coil containing protein kinase 1

(ROCK-1), and Apoptotic protease activating factor 1 (Apaf-1).

Upregulation of cleaved caspase 3,7 were detected by western blot in

addition to Apaf-1 and caspase 9 expressions argues that cell death takes

place via the intrinsic apoptotic pathway in which release of cytochrome

c from the mitochondria triggers the assembly of a caspase activation

complex. We conclude that exposure of Hepa1-6 cells to a low dose of

adjuvanted hepatitis B vaccine leads to loss of mitochondrial integrity,

apoptosis induction, and cell death, apoptosis effect was observed also

in C2C12 mouse myoblast cell line after treated with low dose of vaccine

(0.3, 0.1, 0.05 g/ml). In addition In vivo apoptotic effect of

hepatitis B vaccine was observed in mouse liver.PMID:

22249285 [PubMed - indexed for MEDLINE]

Sheri Nakken, former R.N., MA, Hahnemannian

Homeopath

Vaccination Information & Choice Network, Washington State, USA

Vaccines -

http://vaccinationdangers.wordpress.com/ Homeopathy

http://homeopathycures.wordpress.com

Vaccine Dangers, Childhood Disease Classes & Homeopathy

Online/email courses - next classes start August 2