Re: RESEARCH - Overlapping humoral autoimmunity links rheumatic fever and the antiphospholipid syndrome

November 15, 2006

Yikes, were do you find this stuff and what is

Central

Nervous System abnormalities mean???

Thanks for the info!

Joy

<Matsumura_Clan@...> wrote:

Rheumatology (Oxford). 2006 Jul;45(7):833-41. Epub 2006 May 16.

Overlapping humoral autoimmunity links rheumatic fever and the

antiphospholipid syndrome.

Research Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer

52621, Israel.

OBJECTIVE: Rheumatic fever (RF) and the antiphospholipid syndrome (APS) are

autoimmune diseases that share similar cardiac and neurological pathologies.

We assessed the presence of shared epitopes between M protein,

N-acetyl-beta-D-glucosamine (GlcNAc) and beta2 glycoprotein-I (beta2GPI),

the pathogenic molecules engaged in these autoimmune conditions. METHODS:

Sera from the APS patients were affinity-purified on beta2GPI and

beta2GPI-related peptide columns. Sera from RF patients were

affinity-purified on protein G column. The beta2GPI and M protein-related

peptides were prepared by conventional solid-phase peptide synthesis. The

enzyme-linked immunosorbent assay direct binding and inhibition studies were

performed on the RF and APS sera for the presence, and cross-reactivity, of

antibodies against beta2GPI, beta2GPI-related peptides, streptococcal M

protein, M-derived peptides and GlcNAc. RESULTS: Antibodies (Abs) to

beta2GPI were found in 24.4% of 90 RF patients. Antibodies against various

beta2GPI-related peptides were found in 1.1-36.7% of the patients. The

immunoglobulin G sera from RF patients possessed significant anti-beta2GPI

activity, while sera from APS patients contained a considerable

anti-streptococcal M protein as well as anti-GlcNAc activity. Furthermore,

affinity-purified anti-beta2GPI and anti-beta2GPI-related peptide Abs from

APS patients cross-reacted with streptococcal M protein and M5 peptide,

while beta2GPI and beta2GPI-related peptides inhibited anti-streptococcal M

protein activity from RF patients. The results were confirmed by immunoblot

analyses. The beta2GPI also inhibited anti-GlcNAc activity from APS patients

with chorea.

CONCLUSIONS: The results of our study, showing a considerable overlap of

humoral immunity in RF and APS, support a hypothesis that common pathogenic

mechanisms underlie the development of cardiac valve lesions and Central

Nervous System abnormalities in both diseases.

PMID: 16705050

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed & cmd=Retrieve & dopt=Abstra\

ctPlus & list_uids=16705050

Not an MD

I'll tell you where to go!

Mayo Clinic in Rochester

http://www.mayoclinic.org/rochester

s Hopkins Medicine

http://www.hopkinsmedicine.org

Joy

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

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November 16, 2006

Joy,

I was looking around for a strong link between rheumatic fever and

rheumatoid arthritis, but couldn't find one.

In my searches, I found this article. I posted it because antiphospholipid

antibodies and syndrome affect some of us in the group.

Some people with rheumatic fever (RF) have an associated movement disorder

(chorea - usually temporary) and heart valve damage (may be permanent). Some

people with antiphospholipid syndrome (APS) also experience chorea and have

heart valve involvement. This study found some interesting commonalities

between RF and APS.

Not an MD

I'll tell you where to go!

Mayo Clinic in Rochester

http://www.mayoclinic.org/rochester

s Hopkins Medicine

http://www.hopkinsmedicine.org

Re: [ ] RESEARCH - Overlapping humoral autoimmunity links

rheumatic fever and the antiphospholipid syndrome

> Yikes, were do you find this stuff and what is

> Central

> Nervous System abnormalities mean???

> Thanks for the info!

> Joy

>

> <Matsumura_Clan@...> wrote:

> Rheumatology (Oxford). 2006 Jul;45(7):833-41. Epub 2006 May 16.

>

> Overlapping humoral autoimmunity links rheumatic fever and the

> antiphospholipid syndrome.

>

> Research Center for Autoimmune Diseases, Sheba Medical Center,

> Tel-Hashomer

> 52621, Israel.

>

> OBJECTIVE: Rheumatic fever (RF) and the antiphospholipid syndrome (APS)

> are

> autoimmune diseases that share similar cardiac and neurological

> pathologies.

> We assessed the presence of shared epitopes between M protein,

> N-acetyl-beta-D-glucosamine (GlcNAc) and beta2 glycoprotein-I (beta2GPI),

> the pathogenic molecules engaged in these autoimmune conditions. METHODS:

> Sera from the APS patients were affinity-purified on beta2GPI and

> beta2GPI-related peptide columns. Sera from RF patients were

> affinity-purified on protein G column. The beta2GPI and M protein-related

> peptides were prepared by conventional solid-phase peptide synthesis. The

> enzyme-linked immunosorbent assay direct binding and inhibition studies

> were

> performed on the RF and APS sera for the presence, and cross-reactivity,

> of

> antibodies against beta2GPI, beta2GPI-related peptides, streptococcal M

> protein, M-derived peptides and GlcNAc. RESULTS: Antibodies (Abs) to

> beta2GPI were found in 24.4% of 90 RF patients. Antibodies against various

> beta2GPI-related peptides were found in 1.1-36.7% of the patients. The

> immunoglobulin G sera from RF patients possessed significant anti-beta2GPI

> activity, while sera from APS patients contained a considerable

> anti-streptococcal M protein as well as anti-GlcNAc activity. Furthermore,

> affinity-purified anti-beta2GPI and anti-beta2GPI-related peptide Abs from

> APS patients cross-reacted with streptococcal M protein and M5 peptide,

> while beta2GPI and beta2GPI-related peptides inhibited anti-streptococcal

> M

> protein activity from RF patients. The results were confirmed by

> immunoblot

> analyses. The beta2GPI also inhibited anti-GlcNAc activity from APS

> patients

> with chorea.

>

> CONCLUSIONS: The results of our study, showing a considerable overlap of

> humoral immunity in RF and APS, support a hypothesis that common

> pathogenic

> mechanisms underlie the development of cardiac valve lesions and Central

> Nervous System abnormalities in both diseases.

>

> PMID: 16705050

>

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed & cmd=Retrieve & dopt=Abstra\

ctPlus & list_uids=16705050

November 16, 2006

Thanks again . I see what you are saying.