RESEARCH - Assessment of the clinical significance of gelatinase activity in juvenile idiopathic arthritis

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Ann Rheum Dis. 2006 Apr;65(4):501-7. Epub 2005 Sep 8.

Assessment of the clinical significance of gelatinase activity in patients

with juvenile idiopathic arthritis using quantitative protein substrate

zymography.

Peake NJ, HE, Khawaja K, Cawston TE, Rowan AD.

Musculoskeletal Research Group, School of Clinical Medical Sciences, Medical

School Cookson Building, University of Newcastle upon Tyne, NE2 4HH, UK.

OBJECTIVE: To measure gelatinase activities in paired synovial fluid (SF)

and serum of patients with juvenile idiopathic arthritis (JIA), and to

assess how these activities relate to clinical and laboratory measures of

disease activity. METHODS: A quantitative protein substrate zymography

method was adapted and validated for use with serum and SF. Bands of

activity were measured by densitometry and correlated with standard

laboratory indicators of inflammation: erythrocyte sedimentation rate and

platelet count. RESULTS: Gelatinase activity was found consistently in

patients with JIA, with reproducible, quantified bands of activity

corresponding to pro-matrix metalloproteinase-9 (pro-MMP-9), including the

neutrophil associated lipocalin complex, and pro- and active forms of MMP-2.

Both active MMP-2 and pro-MMP-9 were higher in JIA serum than in controls,

though no differences were seen between patients grouped according to age,

disease duration, or JIA subtype. However, SF MMP-9 correlated significantly

with the laboratory indicators of inflammation, as did the relative level of

active MMP-2.

CONCLUSIONS: Both MMP-2 and MMP-9 gelatinolytic activities are raised during

active JIA and associated with inflammatory activity regardless of age and

disease duration, supporting a role for MMPs in the breakdown of joint

components from early in disease. These MMPs may be specific markers of

active joint destruction linked to inflammatory JIA, MMP-9 as a product of

infiltrating cells, and the activation of MMP-2 produced within the joint.

PMID: 16150790

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve & db=PubMed & list_uids=1\

6150790

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