Guest guest Posted May 24, 2006 Report Share Posted May 24, 2006 I wanted to add something regarding raw milk, though... previous post w/ Cheryl's comments in quotes. Just keep in mind... I believe these are regarding large dairy farms. The other side of the story from pro-raw milk people is that the cows bred for super-milk production and given hormones and feed designed to increase their milk supply (those enormous teats) are first, unhealthy and fed poorly, and the high amount of milk production creates chronic mastitis, and their milk can be so full of puss that they require constant antibiotics, which STILL does not even keep the infections at bay. (Of course, those antiotics cross over into the milk, and then they kill our good flora, too, don't they??) There are many ways to obtain higher nutrition and better enzyme content without giving our kids dairy, though, and that's the area I'm looking into for myself and then my kids. I would, however, consider butters and yogurts and things for myself. Here's the repost: " Since a recent link mentioned raw milk I thought I'd post a few current abstracts on the subject plus a few on BLV. " J Dairy Sci. 2004 Sep;87(9):2822-2830. Prevalence of Salmonellae, Listeria monocytogenes, and Fecal Coliforms in Bulk Tank Milk on US Dairies. Van Kessel JS, Karns JS, Gorski L, McCluskey BJ, Perdue ML. USDA-ARS, Beltsville, MD 20705. The objective of this study was to determine the prevalence of Salmonella, Listeria monocytogenes, and fecal coliforms in bulk tank milk in the United States. As part of the NAHMS Dairy 2002 survey, 861 bulk tank milk samples were collected from farms in 21 states. Milk was directly plated on selective agars for direct bacterial enumeration and was enriched in selective broths to increase detection sensitivity. Somatic cell counts (SCC) and standard plate counts (SPC) were also determined. Coliforms were detected in 95% (818 of 860) of the samples, and the average SCC was 295,000 cells/mL. Twenty-two samples (2.6%) were culture-positive for Salmonella, and 9 serotypes were identified: Montevideo (n = 7), Newport (n = 4), Muenster (n = 2), Meleagridis (n = 2), Cerro (n = 2), 44:Z36 (Z38) (n = 2), Dublin (n = 1), Anatum (n = 1), and 9, 12:nonmo-tile (n = 1). Listeria monocytogenes was isolated from 56 (6.5%) samples, and serotyping of these isolates yielded 5 serotypes (1/2a, 1/2b, 3b, 4b, and 4c). Of the L. monocytogenes isolates, 93% were serotypes 1/2a, 1/2b, and 4b, the most common human clinical isolates. Regional differences in L. monocytogenes and Salmonella prevalence were observed, but more studies are needed to determine the validity of these differences. There were no apparent relationships between SCC or SPC and incidence of Salmonella or L. monocytogenes. Although the prevalence of L. monocytogenes and Salmonella was low, these pathogens represent a potential risk to consumers of raw milk and raw milk products. PMID: 15375040 [PubMed - as supplied by publisher] : Appl Environ Microbiol. 2004 Sep;70(9):5644-50. Related Articles, Links Raw cow milk bacterial population shifts attributable to refrigeration. Lafarge V, Ogier JC, Girard V, Maladen V, Leveau JY, Gruss A, Delacroix-Buchet A. Unite Composition et Hygiene des Produits Laitiers, Agence Francaise de Securite Sanitaire des Aliments, 39-41 rue du 11 novembre 1918, 94700 Maisons Alfort, France. v.lafarge@... We monitored the dynamic changes in the bacterial population in milk associated with refrigeration. Direct analyses of DNA by using temporal temperature gel electrophoresis (TTGE) and denaturing gradient gel electrophoresis (DGGE) allowed us to make accurate species assignments for bacteria with low-GC-content (low-GC%) (<55%) and medium- or high-GC% (>55%) genomes, respectively. We examined raw milk samples before and after 24-h conservation at 4 degrees C. Bacterial identification was facilitated by comparison with an extensive bacterial reference database ( approximately 150 species) that we established with DNA fragments of pure bacterial strains. Cloning and sequencing of fragments missing from the database were used to achieve complete species identification. Considerable evolution of bacterial populations occurred during conservation at 4 degrees C. TTGE and DGGE are shown to be a powerful tool for identifying the main bacterial species of the raw milk samples and for monitoring changes in bacterial populations during conservation at 4 degrees C. The emergence of psychrotrophic bacteria such as Listeria spp. or Aeromonas hydrophila is demonstrated. PMID: 15345453 [PubMed - in process] Annu Rev Microbiol. 2004 Jun 2 [Epub ahead of print] Related Articles, Links e's Disease, Inflammatory Bowel Disease, and Mycobacterium paratuberculosis. Chacon O, Bermudez LE, Barletta RG. Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Lincoln, NE 68588; Seccion de Bacteriologia Corporacion para Investigaciones Biologicas (CIB), Carrera, Medellin, Colombia ochacon@..., Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331 luiz.bermudez@..., Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Lincoln, NE 68588-0905 rbarletta@... e's disease is a chronic diarrhea affecting all ruminants. Mycobacterium avium subsp. paratuberculosis (MAP), a slow-growing mycobacteria, is the etiologic agent. There is also a concern that MAP might be a causative agent of some cases of inflammatory bowel disease in human beings, especially Crohn's disease. Food products including pasteurized bovine milk have been suggested as potential sources of human infection. This review addresses microbial factors that may contribute to its pathogenicity. In addition, the experimental evidence defining MAP as the cause of e's disease and, the issues and controversies surrounding its potential pathogenic role in human beings are discussed. Expected online publication date for the Annual Review of Microbiology Volume 58 is September 8, 2004. Please see http://www.annualreviews.org/catalog/pub_dates.asp for revised estimates. PMID: 15355186 [PubMed - as supplied by publisher] Prev Vet Med. 2003 Dec 12;61(4):249-62. Related Articles, Links Association between bovine-leukosis virus seroprevalence and herd-level productivity on US dairy farms. Ott SL, R, Wells SJ. USDA, Centers for Epidemiology and Animal Health, 2150 Centre Avenue, Building B, Mail Stop 2E5, Fort , CO 80526-8117, USA. stephen.l.ott@... Bovine-leukosis virus (BLV; also termed 'bovine-leukemia virus') is a retrovirus that primarily affects lymphoid tissue of dairy and beef cattle. Our objective was to investigate the association between BLV infection and annual value of production (AVP) on dairy herds within the United States, as part of the USDA National Animal Health Monitoring System's 1996 dairy study. 1006 herds (in 20 states) with at least 30 dairy cows were interviewed during 1996. The agar-gel immunodiffusion test was used to detect serum antibodies to BLV. 10-40 cows from each herd were tested and each tested cow was classified as negative or positive based on results of a single test. A multivariable regression model was used with the 976 herds with complete data for analysis. When compared to herds with no test-positive cows, herds with test-positive cows produced 218 kg per cow (i.e. 3%) less milk. The average reduction in AVP was $59 per cow for test-positive herds relative to test-negative herds. For the dairy industry as a whole, BLV seropositivity was associated with loss to producers of $285 million and $240 million for consumers. Most of this $525 million industry loss was due to reduced milk production in test-positive herds. PMID: 14623410 [PubMed - indexed for MEDLINE] AIDS Res Hum Retroviruses. 2003 Dec;19(12):1105-13. Humans have antibodies reactive with Bovine leukemia virus. Buehring GC, Philpott SM, Choi KY. School of Public Health, University of California, Berkeley, California 94720, USA. buehring@... Bovine leukemia virus (BLV) is an oncogenic retrovirus that commonly infects cattle and causes B cell leukosis in 1-5% of infected cattle. BLV-infected cells are present in marketed beef and dairy products. In the decade after the discovery of BLV in 1969, studies using agar gel immunodiffusion and complement fixation assays failed to find antibodies to BLV in human sera. This led to the prevailing opinion that exposure of humans to BLV and/or the potential for infection are not significant and therefore the virus is not a public health hazard. We reexamined this issue using more sensitive immunological techniques available today. Using immunoblotting to test the sera of 257 humans for antibodies of four isotypes (IgG1, IgM, IgA, and IgG4) to the BLV capsid antigen (p24), we detected at least one antibody isotype reactive with BLV in 74% of the human sera tested. The specificity of the reactivity was strongly suggested by competition studies and by ruling out cross-reacting antibodies to other chronic human viruses. Our results suggest that antibodies reactive with the BLV capsid antigen may serve as a biomarker for exposure to BLV and this exposure may be widespread. The results do not necessarily mean that humans are actually infected with BLV; the antibodies could be a response to heat-denatured BLV antigens consumed in food. They do, however, suggest that further studies in this area could be important. PMID: 14709247 [PubMed - indexed for MEDLINE] __________________________________________________ Quote Link to comment Share on other sites More sharing options...
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