Hepatitis B vaccine induces apoptotic death, loss of mitochondrial integrity

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Hepatitis B vaccine induces apoptotic death in Hepa1-6 cells.

<http://www.ncbi.nlm.nih.gov/pubmed/22249285>

Hamza H, Cao J, Li X, Li C, Zhu M, Zhao S.

Apoptosis. 2012 May;17(5):516-27.

Vaccines can have adverse side-effects, and these are predominantly

associated with the inclusion of chemical additives such as aluminum

hydroxide adjuvant. The objective of this study was to establish an in

vitro model system amenable to mechanistic investigations of

cytotoxicity induced by hepatitis B vaccine, and to investigate the

mechanisms of vaccine-induced cell death. The mouse liver hepatoma cell

line Hepa1-6 was treated with two doses of adjuvanted (aluminium

hydroxide) hepatitis B vaccine (0.5 and 1 ?g protein per ml) and cell

integrity was measured after 24, 48 and 72 h.... We conclude that

exposure of Hepa1-6 cells to a low dose of adjuvanted hepatitis B

vaccine leads to loss of mitochondrial integrity, apoptosis induction,

and cell death, apoptosis effect was observed also in C2C12 mouse

myoblast cell line after treated with low dose of vaccine (0.3, 0.1,

0.05 ?g/ml). In addition In vivo apoptotic effect of hepatitis B vaccine

was observed in mouse liver.