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adjuvant aluminium hydroxide: Hepatitis B vaccine induces apoptotic death in hepatic cells

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Hepatitis B vaccine induces apoptotic death in Hepa1-6 cells.

<http://www.ncbi.nlm.nih.gov/pubmed/22249285>

Hamza H, Cao J, Li X, Li C, Zhu M, Zhao S.

Apoptosis. 2012 Jan 17. [Epub ahead of print]

Vaccines can have adverse side-effects, and these are predominantly

associated with the inclusion of chemical additives such as aluminum

hydroxide adjuvant. The objective of this study was to establish an in

vitro model system amenable to mechanistic investigations of

cytotoxicity induced by hepatitis B vaccine, and to investigate the

mechanisms of vaccine-induced cell death. The mouse liver hepatoma cell

line Hepa1-6 was treated with two doses of adjuvanted (aluminium

hydroxide) hepatitis B vaccine (0.5 and 1 ?g protein per ml) and cell

integrity was measured after 24, 48 and 72 h. Hepatitis B vaccine

exposure increased cell apoptosis as detected by flow cytometry and

TUNEL assay. Vaccine exposure was accompanied by significant increases

in the levels of activated caspase 3, a key effector caspase in the

apoptosis cascade. Early transcriptional events were detected by

qRT-PCR. We report that hepatitis B vaccine exposure resulted in

significant upregulation of the key genes encoding caspase 7, caspase 9,

Inhibitor caspase-activated DNase (ICAD), Rho-associated coiled-coil

containing protein kinase 1 (ROCK-1), and Apoptotic protease activating

factor 1 (Apaf-1). Upregulation of cleaved caspase 3,7 were detected by

western blot in addition to Apaf-1 and caspase 9 expressions argues that

cell death takes place via the intrinsic apoptotic pathway in which

release of cytochrome c from the mitochondria triggers the assembly of a

caspase activation complex. We conclude that exposure of Hepa1-6 cells

to a low dose of adjuvanted hepatitis B vaccine leads to loss of

mitochondrial integrity, apoptosis induction, and cell death, apoptosis

effect was observed also in C2C12 mouse myoblast cell line after treated

with low dose of vaccine (0.3, 0.1, 0.05 ?g/ml). In addition In vivo

apoptotic effect of hepatitis B vaccine was observed in mouse liver.

- - - -

New Study: Hepatitis B Vaccine Damages The Liver

http://www.thehealthyhomeeconomist.com/new-study-hepatitis-b-vaccine-damages-the\

-liver/

When Jodi Ferris unknowingly unleashed the wrath of Social Services at

Penn State Hershey Medical Center by questioning the necessity of the

Hepatitis B vaccine for her newborn daughter, little did she know just

how big a can of worms she was opening.

A study reported in the journal Apoptosis just weeks ago indicates that

this controversial vaccine normally injected into newborns within hours

of birth induces liver damage primarily due to the presence of the toxic

vaccine adjuvant aluminum hydroxide....

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