Oxidative Stress

January 10, 2002

> Hello Andy or anyone else:

>

> I read in a recent post where you mentioned oxidative stress caused

by

> the chelation process. How is this stress manifested in behavior?

As a worsening of the signs and symptoms of whatever the problem is.

E. g. autism, PDD, ADHD.

> I've been chelating for about 20 weeks. First half with 100mg DMSA

and

> 2nd half with 100 DMSA/25 ALA. My son is 65 lbs 10 yrs old and

ADHD.

> We've been doing the 3hr day 4 hr night 2.5 days on 4.5 days off.

>

> When we first added the ALA he did have some blisters in his mouth

and I

> believe the second round he also had a ring around his anus that was

red

> and soar. All these symptoms appeared in the first three phase 2

rounds

> and haven't seen them since.

>

> What I've noticed lately was Quinton having a really hard time

focussing

> in school such that he could get his work done in class so he would

have

> to bring it home plus do his homework. It would take him all night

long

> to get it done. I would have to sit with him in the room and help

him to

> get through it. He was also having small outburst of anger over

things

> that had not normally been bothering him.

>

> I've been taking a break to give him a rest for the last three

weeks.

If he improves during this break it indicates he got a little too much

stress during chelation and is recovering from it.

> I'm also going to have all the baseline tests run that we did before

we

> got started (Complete Met Panel, TSH, T3, T4, FT4, CBC, Cortisol,

Total

> T3, ACTH, and IGE). Is there anything else that I should check?

I can't think of anything unless there is a specific need for it.

> Thanks for you help and comments

>

> In Christ's love an

May 29, 2002

Hi Robbin, I thought that this might help with your research.

MY MOMS SIDE OF THE FAMILY

There was no arthritis on my moms side of the family.

MY DADS SIDE OF THE FAMILY There is a lot of RA on my dads side of the family. On my dads side, his dad(my grandpa) DID NOT have any arthritis. But all of my dads(my grandpa) sisters DID HAVE RA(every one of them). My dads mom(my grandma) she had RA(and diabetes) and all of her sisters had RA too (just like my grandpas sisters did) None of my dads sisters or brothers have arthritis but my dad does have some type and I'm not sure, all I know is they found that he has spurs in his back which I think would be osteo.And he also has TMJ. Nor my sister or brother has arthrits(yet) but I do. I have spinal stenosis,myofacial pain syndrome,tmj, and fibromyalgia.

BRIANNAS DADS SIDE OF THE FAMILY

On nas dads() side of the family- (s moms side)s mom, grandma,and aunt have osteo arthritis. And s great aunt and great grandfather had RA (on s moms side).

LESLIES DADS SIDE OF THE FAMILY

On leslies dads(nas grandpa) side of the family- s Third cousin has systemic JRA and we are not sure how old she was when she got it because nas dad never really asociated with that side of his family very much but s mom(briannas grandma) called a relative whom is very old and dosn't have much of a memory and all the lady new was that she had gotten it when she was really young and the little girl is now 11 years old. We didn't find this out until here recently when s mom was talking to a friend at work telling her about nas disease and stuff and her friend happened to know the little girls family and then they both realized that it was actually s 3rd cousin who had the systemic JRA.

and that is our family history on arthritis and I hope it will help.

Jona mom of na 2 poly

Robbin40@... wrote: Duncan ok nutrients is very important if you look at girls as example you can tell those who eat more meat than veggies girls who eat meat more will develop faster than those who eat mostly veggies proven to me by the kids around this house my daughter isnt a meat eater all the time and she will eat chicken more than any other meat but she loves veggies my neighbor who is a month younger than my daughter developed very early and she didnt like it at all then my daughter felt left out because all the girls had developed before her and she was the oldest you can also use tell tell signs in boys also from what i researched on this is that the FDA approves the beef to have steroid injections caompare to a boy and girl raised on a farm where theres no steroid injection and you will see the difference its often to late to understand i use to give my daughter a vitamin everyday later stopped because of the ingredients i make sure that she eats enough of natural vitamins through foods the MSG i researched again when she started having severe headaches thinking she was allergic to some other type of foods come to find out the foods that had MSG was the ones that she reacted to along with a pseudo tumor celebri now she doesnt just look at the picture and say she wants this she actually READS it and if it has MSG she will not even get it lolol that meant giving up her favorite food mac and cheese the powder cheese has MSG in it bummer for her but she has understood my point on how the food process goes pesticides also is bad Duncan so you see why i did the research and still do research on things if its in the gene line where did it run or how am i the one carrier or is it my hubby or is it a grand parent thing i have seen the parkinsons disease travel and its every generation and the color of hair that person had whom got it at least thats the way it did in the family what could be triggers? chromos line could it be the X Y or the X X thing? i know in this family which is strange that my 3 sisters were allergic to the MMR shot and then my daughter was allergic to MMR shot didnt know this until later about my sisters also being allergic tracing things is fun but also fustrating this is why i do family research also and go looking for cause of death and family history epilepssy my dad had which can jump a generation but so far me nor my sister has that and since she doesnt have kids and i do neither of my kids got it but its a line stemming there there needs to be a parent survey to which these questions could be answered by parents of JRA kids all over the world to at least get a base of things possible to take the chromo test from both parents and the child to see if theres a gene positive the rhuemy doctors could start this i am not sure what you are familar with but i have alot of experience in southern foods some foods your only to eat at certain times of the year poke salad this plant will poison you if not picked the right way from the top you can only eat the first 3 leaves top down then theres kudzu i have even read some on indian ways and religous beliefs this still amazes me i will read the foods of the earth theres something about the persimon tree fruit cranberry juice is great for many things also apple juice is good also and so is prune juice to detox the body you have to drink only liquified stuff well sorry for this being long i have to get melissa bathed talk later Robbin

October 31, 2003

Sue,

I think that so far the direct evidence of oxidative stress in CFS

is for red blood cells, blood plasma, skeletal muscles and liver. I

think there is probably oxidative stress in other tissues as well,

such as in the nervous system, but I don't think direct measurements

have been made there. I guess brain biopsies are not too popular....

(;-)

Rich

> Hi Rich,

>

> >...in CFS there is a condition of oxidative stress...

>

> Is this just in red blood cells or every cell in the body or just

certain

> groups of cells in particular tissues?

>

> Sue B.

> Upstate New York

December 18, 2003

You'll have to check your medical websites for the exact definition of this.

The body recovers from it, but you have to give it rest.

You don't want to get too much mineral depletion. Kids get TIRED and cranky

from chelating (usually on the last day or the next) so the body needs some

time to recoup. Remember, you are pulling mercury through the brain and

body--that's work! Some people say it feels like having a mild flu.

On the positive side, many kids go through this, and after each cycle begin

to " come back " to their parents. I've been on this list for over three

years and have heard many wonderful stories of recovery. So chelation is

something that needs to be done carefully (not in a hurry) and safely, but

often brings great benefit. Read the files of this list for more

information.

Barb

[ ] Oxidative Stress

> What Oxidative Stress is?

>

> If I do not take enough supplement on the chelation cycle, what would

> happen to the body?

>

> =======================================================

>

December 24, 2003

Basically it is rancidity. It is what makes dried fruit turn all kinds

of icky colors if you don't use sulfite on it.

Andy

> What Oxidative Stress is?

>

> If I do not take enough supplement on the chelation cycle, what would

> happen to the body?

September 19, 2006

I wonder how much various forms of mercury in one's body can affect the levels of these markers. Aasa http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve & db=PubMed & list_uids=6226254 & dopt=Abstract http://molpharm.aspetjournals.org/cgi/content/abstract/19/3/470 http://pharmrev.aspetjournals.org/cgi/reprint/52/1/113.pdf#search=%22thromboxane%20ethyl%20mercury%22 http://www.medscape.com/medline/abstract/2495003max walker

<max.walker@...> wrote: D Penn Researchers Find Link Between Autism and Abnormal Blood-Vessel Function and Oxidative

Stress New Findings Could Help Explain Pathology of Autistic Syndrome (Philadelphia, PA) - Researchers at the University of Pennsylvania School of Medicine discovered that children with autism showed signs of abnormal blood-vessel function and damaging levels of oxidative stress compared to healthy children. The children with autism possessed levels of biochemicals that indicate the presence of constricted blood

vessels via the endothelium (the cells that line vessels) with a higher tendency to form clots (through cells called platelets). By exploring the relationship between oxidative stress and blood-vessel function in autistic patients, investigators hope to find new therapeutic options for this syndrome. The researchers, led by Domenico Pratico, MD, Associate Professor of Pharmacology, published their findings in the August issue of the Archives of Neurology. According to the Autism Society of America, the reported number of autism cases is increasing 10 to 17 percent per

year in the United States. Autism, an early onset neurological disorder, is characterized by impaired social interactions, limited verbal and nonverbal communication, and repetitive and restricted behavioral patterns. Patients with autism can differ in the severity and scope of their symptoms, suggesting that multiple factors contribute to explaining the disorder's symptoms. Previous studies at other institutions have shown that autistic patients have reduced cerebral blood flow, presumably due to constricted blood vessels in the brain, versus healthy controls. Urinary samples of autistic children who were similar in age and healthy controls were provided by the Pfeiffer Treatment Center (www.hriptc.org/), where patients were diagnosed with autism disorder and

evaluated. Patients were excluded from analysis if they had ever received anti-oxidant treatments or medicine with any known anti-oxidant effect; if they suffered from chronic illnesses, such as depression, psychosis, or inflammatory disorders; and/or if they were sick at the time of the sample collection. These strict criteria resulted in the small sample size in this preliminary study: 26 children with autism and 12 healthy controls. Pratico's team measured isoprostane, a biomarker for oxidative stress; thromboxane, an index of platelet activation; and prostacyclin, a measure of blood vessel activation in the samples. "This study represents the first observation that the rates of thromboxane and

prostacyclin synthesis are both not only significantly increased in autism, but are closely correlated with the rate of oxidative stress," says Pratico. Compared with controls, children with autism had significantly higher urinary levels of isoprostane, thromboxone, and prostacyclin. Oxidative stress is the result of an excessive formation of chemically unstable byproducts, called free radicals, within the cell. Under normal conditions, the cell is able to destroy the free radicals. However, when excessive free radicals accumulate, these molecules mount an attack against the cell in search of chemical stability. "During oxidative stress, it is as if the free radicals have only one leg,"

explains Pratico. "They are searching for the second leg in order to keep from falling. Unfortunately, the ability of the excessive free radicals to reestablish their chemical equilibrium comes always with a price for the organ -- irreversible cellular and organ damage." Free radicals can damage cell membranes, proteins, and genes by oxidation -- the same chemical reaction that causes iron to rust. Pratico and colleagues measured levels of isoprostane, the chemical byproduct of free radicals attacking fat cells and found that patients with autism possess nearly double the level of oxidative stress than that measured in healthy controls. The samples from autistic patients also revealed a

biochemical imbalance in the patients' blood vessels, resulting in high levels of thromboxane â€“ an indicator of platelet activity â€“ and prostacyclin, an indicator of constricting endothelial cells. During normal function, thromboxane and prostacyclin work together to maintain the integrity of vessels. In response to different kinds of stress, platelets release thromboxane, which causes vessels to contract. The endothelium responds to elevated levels of thromboxane by releasing prostacyclin. This event counterbalances the effect on vessels, inducing dilation of the vessel and, in turn, more blood flow. Autism is a complex neurological disorder and oxidative imbalance is one feature of the autistic syndrome. Several lines of evidence support the hypothesis that oxidative imbalance may also play a role in this disease: autism is

characterized by an impaired anti-oxidant defense system, higher free-radical production, and improvement of behavioral symptoms after taking anti-oxidants. "In general, it is known that abnormalities in blood vessels can be clinically reflected by an abnormal blood flow," says Pratico. "In this regard, it is interesting that earlier neuroimaging studies of autistic children have demonstrated a reduced amount of blood reaching the brain. Shedding more light on the relationship of oxidative stress and blood-vessel health to the pathology of autism could lead to improvements in therapy." ###Study co-authors are Yuemang Yao from Penn; J. Walsh, Pfeiffer

Treatment Center (Warrenville, IL); and Woody R. McGinnis, Oxidative Stress in Autism Initiative (Ashland,OR). The research was supported in part by the Pfeiffer Treatment Center.This release can also be seen at: www.uphs.upenn.edu/news.

September 19, 2006

He could probably walk across the hall and visit his colleage Dr

Offit to figure out where the oxidative stress is coming from ....

>

> D

> Penn Researchers Find Link Between Autism and Abnormal

>

> Blood-Vessel Function and Oxidative Stress

>

> New Findings Could Help Explain Pathology of Autistic Syndrome

>

> (Philadelphia, PA) - Researchers at the University of Pennsylvania

School of Medicine discovered that children with autism showed signs

of abnormal blood-vessel function and damaging levels of oxidative

stress compared to healthy children. The children with autism

possessed levels of biochemicals that indicate the presence of

constricted blood vessels via the endothelium (the cells that line

vessels) with a higher tendency to form clots (through cells called

platelets).

>

> By exploring the relationship between oxidative stress and blood-

vessel function in autistic patients, investigators hope to find new

therapeutic options for this syndrome. The researchers, led by

Domenico Pratico, MD, Associate Professor of Pharmacology, published

their findings in the August issue of the Archives of Neurology.

>

> According to the Autism Society of America, the reported number of

autism cases is increasing 10 to 17 percent per year in the United

States. Autism, an early onset neurological disorder, is

characterized by impaired social interactions, limited verbal and

nonverbal communication, and repetitive and restricted behavioral

patterns. Patients with autism can differ in the severity and scope

of their symptoms, suggesting that multiple factors contribute to

explaining the disorder's symptoms. Previous studies at other

institutions have shown that autistic patients have reduced cerebral

blood flow, presumably due to constricted blood vessels in the

brain, versus healthy controls.

>

> Urinary samples of autistic children who were similar in age and

healthy controls were provided by the Pfeiffer Treatment Center

(www.hriptc.org/), where patients were diagnosed with autism

disorder and evaluated. Patients were excluded from analysis if they

had ever received anti-oxidant treatments or medicine with any known

anti-oxidant effect; if they suffered from chronic illnesses, such

as depression, psychosis, or inflammatory disorders; and/or if they

were sick at the time of the sample collection. These strict

criteria resulted in the small sample size in this preliminary

study: 26 children with autism and 12 healthy controls.

>

> Pratico's team measured isoprostane, a biomarker for oxidative

stress; thromboxane, an index of platelet activation; and

prostacyclin, a measure of blood vessel activation in the

samples. " This study represents the first observation that the rates

of thromboxane and prostacyclin synthesis are both not only

significantly increased in autism, but are closely correlated with

the rate of oxidative stress, " says Pratico. Compared with controls,

children with autism had significantly higher urinary levels of

isoprostane, thromboxone, and prostacyclin.

>

> Oxidative stress is the result of an excessive formation of

chemically unstable byproducts, called free radicals, within the

cell. Under normal conditions, the cell is able to destroy the free

radicals. However, when excessive free radicals accumulate, these

molecules mount an attack against the cell in search of chemical

stability.

>

> " During oxidative stress, it is as if the free radicals have only

one leg, " explains Pratico. " They are searching for the second leg

in order to keep from falling. Unfortunately, the ability of the

excessive free radicals to reestablish their chemical equilibrium

comes always with a price for the organ -- irreversible cellular and

organ damage. " Free radicals can damage cell membranes, proteins,

and genes by oxidation -- the same chemical reaction that causes

iron to rust.

>

> Pratico and colleagues measured levels of isoprostane, the

chemical byproduct of free radicals attacking fat cells and found

that patients with autism possess nearly double the level of

oxidative stress than that measured in healthy controls.

>

> The samples from autistic patients also revealed a biochemical

imbalance in the patients' blood vessels, resulting in high levels

of thromboxane â? " an indicator of platelet activity â? " and

prostacyclin, an indicator of constricting endothelial cells. During

normal function, thromboxane and prostacyclin work together to

maintain the integrity of vessels. In response to different kinds of

stress, platelets release thromboxane, which causes vessels to

contract. The endothelium responds to elevated levels of thromboxane

by releasing prostacyclin. This event counterbalances the effect on

vessels, inducing dilation of the vessel and, in turn, more blood

flow.

>

> Autism is a complex neurological disorder and oxidative imbalance

is one feature of the autistic syndrome. Several lines of evidence

support the hypothesis that oxidative imbalance may also play a role

in this disease: autism is characterized by an impaired anti-oxidant

defense system, higher free-radical production, and improvement of

behavioral symptoms after taking anti-oxidants.

>

> " In general, it is known that abnormalities in blood vessels can

be clinically reflected by an abnormal blood flow, " says

Pratico. " In this regard, it is interesting that earlier

neuroimaging studies of autistic children have demonstrated a

reduced amount of blood reaching the brain. Shedding more light on

the relationship of oxidative stress and blood-vessel health to the

pathology of autism could lead to improvements in therapy. "

>

> ###

> Study co-authors are Yuemang Yao from Penn; J. Walsh,

Pfeiffer Treatment Center (Warrenville, IL); and Woody R. McGinnis,

Oxidative Stress in Autism Initiative (Ashland,OR). The research was

supported in part by the Pfeiffer Treatment Center.

> This release can also be seen at: www.uphs.upenn.edu/news.

>

> -------------------------------------------------------------------

-------------

>

September 20, 2006

Note: forwarded message attached.