Deep sequencing reveals viral vaccine contaminants

[Guest guest]

Deep sequencing reveals viral vaccine contaminants

The sequence analysis revealed the expected vaccine strains in each

preparation, and in three cases, other unexpected viral sequences. The

retrovirus avian leukosis virus was found in the measles vaccine, but at a

very low level (700 nucleotides from 4 sequence reads). A virus similar to

simian retrovirus was identified in Rotateq (276 nucleotides from 1 sequence

read). Significant levels of porcine cirovirus 1 were found in Rotarix. The

entire viral genome sequence was deduced from 6344 sequence reads,

comprising over 40% of the reads done for that vaccine.

The avian leukosis virus sequences found in the measles vaccine are in

intact virions, as DNA treatment of the vaccines did not prevent their

detection by polymerase chain reaction (PCR). However simian retrovirus DNA

was not detected by PCR after DNAse treatment of Rotateq. Therefore the

viral DNA detected in Rotateq vaccine most likely originates from host DNA

present in the vaccine preparation. The cells used to produce Rotateq are

Vero cells ­ African green monkey kidney cells. A defective form of simian

retrovirus DNA, called proviral DNA, is integrated into Vero cell DNA....

http://www.forums.aboutmecfs.org/showthread.php?3753-Mouse-retrovirus-presen

t-in-cell-lines-used-for-production-of-biologicals & p=66385 & posted=1#post6638

5

[Guest guest]

Natasa,

 

Again, I am so glad you are back. Please correct me if I am wrong but is not the

African Green Monkey used in other vaccinations such as Hep B? Could viruses be

transmitted in other vaccines that are not live? I have to go back to my book,

the River, but I think the author touched on this subject where vaccines that

were suppose to be not activated actually transmitted live viruses.

 

Thank you,

 

Jill

From: Neno/Natasa <neno@...>

Subject: Deep sequencing reveals viral vaccine contaminants

" " < >

Date: Friday, April 9, 2010, 5:25 AM

 

Deep sequencing reveals viral vaccine contaminants

The sequence analysis revealed the expected vaccine strains in each

preparation, and in three cases, other unexpected viral sequences. The

retrovirus avian leukosis virus was found in the measles vaccine, but at a

very low level (700 nucleotides from 4 sequence reads). A virus similar to

simian retrovirus was identified in Rotateq (276 nucleotides from 1 sequence

read). Significant levels of porcine cirovirus 1 were found in Rotarix. The

entire viral genome sequence was deduced from 6344 sequence reads,

comprising over 40% of the reads done for that vaccine.

The avian leukosis virus sequences found in the measles vaccine are in

intact virions, as DNA treatment of the vaccines did not prevent their

detection by polymerase chain reaction (PCR). However simian retrovirus DNA

was not detected by PCR after DNAse treatment of Rotateq. Therefore the

viral DNA detected in Rotateq vaccine most likely originates from host DNA

present in the vaccine preparation. The cells used to produce Rotateq are

Vero cells ­ African green monkey kidney cells. A defective form of simian

retrovirus DNA, called proviral DNA, is integrated into Vero cell DNA....

http://www.forums. aboutmecfs. org/showthread. php?3753- Mouse-retrovirus

-presen

t-in-cell-lines- used-for- production- of-biologicals & p=66385 & posted=

1#post6638

5

[Guest guest]

>

>

> I don’t think it matters weather the vaccine is live or not

>

>

>

> Natasa,

>  

> Again, I am so glad you are back. Please correct me if I am wrong but is not

> the African Green Monkey used in other vaccinations such as Hep B? Could

> viruses be transmitted in other vaccines that are not live? I have to go back

> to my book, the River, but I think the author touched on this subject where

> vaccines that were suppose to be not activated actually transmitted live

> viruses.

>  

> Thank you,

>  

> Jill

>

>

>

> From: Neno/Natasa <neno@...

> <mailto:neno%40dalmaholidays.co.uk> >

> Subject: Deep sequencing reveals viral vaccine contaminants

> " <mailto:%40> "

> < <mailto:%40> >

> Date: Friday, April 9, 2010, 5:25 AM

>

>  

>

> Deep sequencing reveals viral vaccine contaminants

>

> The sequence analysis revealed the expected vaccine strains in each

> preparation, and in three cases, other unexpected viral sequences. The

> retrovirus avian leukosis virus was found in the measles vaccine, but at a

> very low level (700 nucleotides from 4 sequence reads). A virus similar to

> simian retrovirus was identified in Rotateq (276 nucleotides from 1 sequence

> read). Significant levels of porcine cirovirus 1 were found in Rotarix. The

> entire viral genome sequence was deduced from 6344 sequence reads,

> comprising over 40% of the reads done for that vaccine.

>

> The avian leukosis virus sequences found in the measles vaccine are in

> intact virions, as DNA treatment of the vaccines did not prevent their

> detection by polymerase chain reaction (PCR). However simian retrovirus DNA

> was not detected by PCR after DNAse treatment of Rotateq. Therefore the

> viral DNA detected in Rotateq vaccine most likely originates from host DNA

> present in the vaccine preparation. The cells used to produce Rotateq are

> Vero cells ­ African green monkey kidney cells. A defective form of simian

> retrovirus DNA, called proviral DNA, is integrated into Vero cell DNA....

>

> http://www.forums. aboutmecfs. org/showthread. php?3753- Mouse-retrovirus

> -presen

> t-in-cell-lines- used-for- production- of-biologicals & p=66385 & posted=

> 1#post6638

> 5

>

>