Oldie on Culturelle & why it might irritate PANDAS

[Guest guest]

Now for some people, this may not be so bad.  Sometimes stimulation is good -

depending on what type of illness.  I think it depends on someone's immune

panel. 

But for PANDAS, OCD, anxiety... whoa.  I think TNFa is already associated with

those, and some of the other inflammatory cytokines/chemokines/interleukins.

This is only a small collection of them.

  

Culturelle is manufactured in gelatin capsules

providing 20 billion colony forming units (CFUs) per

capsule of Lactobacillus GG.

J Leukoc Biol. 2003 Sep; 74(3): 395-402.

Lactobacilli and streptococci induce inflammatory

chemokine production in human macrophages that

stimulates Th1 cell chemotaxis.

Veckman V, Miettinen M, Matikainen S, Lande R,

Giacomini E, Coccia EM, Julkunen I.

Department of Microbiology, National Public Health

Institute, Helsinki,

Finland. ville.veckman@...

Macrophages have a central role in innate-immune

responses to bacteria.

In the present work, we show that infection of human

macrophages with

Gram-positive pathogenic Streptococcus pyogenes or

nonpathogenic Lactobacillus rhamnosus GG enhances mRNA

expression of inflammatory chemokine ligands

CCL2/monocyte chemoattractant protein-1 (MCP-1),

CCL3/macrophage-inflammatory protein-1alpha

(MIP-1alpha), CCL5/regulated on activation, normal T

expressed and secreted, CCL7/MCP-3, CCL19/MIP-3beta,

and CCL20/MIP-3alpha and CXC chemokine igands

CXCL8/interleukin (IL)-8, CXCL9/monokine induced by

interferon-gamma (IFN-gamma), and CXCL10/IFN-inducible

protein 10. Bacteria-induced CCL2, CCL7, CXCL9, and

CXCL10 mRNA expression was partially dependent on

ongoing protein synthesis.

The expression of these chemokines and of CCL19 was

dependent on bacteria-induced IFN-alpha/beta

production. CCL19 and CCL20 mRNA expression was

up-regulated by IL-1beta or tumor necrosis factor

alpha

(TNF-alpha), and in addition, IFN-alpha together with

TNF-alpha further enhanced CCL19 gene expression.

Synergy between IFN-alpha and TNF-alpha was also seen

for CXCL9 and CXCL10 mRNA expression.

Bacteria-stimulated macrophage supernatants induced

the migration of T helper cell type 1 (Th1) cells,

suggesting that in human macrophages, these bacteria

can stimulate efficient inflammatory chemokine gene

expression including those that recruit Th1 cells to

the site of inflammation. Furthermore, L.

rhamnosus-induced Th1 chemokine production could in

part explain the proposed antiallergenic properties of

this bacterium.

PMID: 12949243 [PubMed - indexed for MEDLINE]

J Food Prot. 2003 Mar; 66(3): 466-72.  Links

Interactions of lactic acid bacteria with human

intestinal epithelial cells: effects on cytokine

production.

Wallace TD, Bradley S, Buckley ND, Green- JM.

Department of Biology, Acadia University, Wolfville,

Nova Scotia,

Canada B0P 1X0.

As a participant in the mucosal immune response, the

intestinal epithelial cell must respond to a variety

of stimuli, including lactic acid bacteria (LAB)

consumed in the diet. The objective of this study was

to compare the abilities of several strains of LAB to

modulate cytokine secretion by human intestinal

epithelial cell (IEC) line HT-29. Certain strains of

Lactobacillus rhamnosus, Lactobacillus delbrueckii,

and Lactobacillus acidophilus suppressed the

production of the chemokine RANTES by stimulated HT-29

IEC, although the magnitude of this suppression varied

depending on the nature of the bacterial growth

medium. Similarly, specific strains showed growth

condition-dependent suppression of HT-29 interleukin-8

(IL-8) production. Strain-dependent effects were also

seen for the suppression of tumor necrosis factor

alpha (TNF-alpha) and transforming growth factor beta

(TGF-beta) production. The binding of several of these

bacterial strains to the HT-29 cell line was also

examined. Different strains were found to have

differing abilities to interact with IEC, with L.

rhamnosus R0011 being the strain that generally had

the most extensive effects on HT-29 cytokine

production and also bound to HT-29 IEC most

effectively. Modulation of IEC cytokine production has

the potential to profoundly affect the mucosal

microenvironment, influencing the immune response to

pathogens and other ingested antigens.

PMID: 12636302 [PubMed - indexed for MEDLINE]

:  Salminen MK, Rautelin H, Tynkkynen S, Poussa T,

Saxelin M, Valtonen

V,

Jarvinen A.

Lactobacillus bacteremia, clinical significance, and

patient outcome, with special focus on probiotic L.

rhamnosus GG.

Clin Infect Dis. 2004 Jan 1; 38(1): 62-9. Epub 2003

Dec 04.

PMID: 14679449 [PubMed - in process]

Scand J Infect Dis. 2003; 35(6-7): 404-8.

Six cases of Lactobacillus bacteraemia: identification

of organisms and antibiotic susceptibility and

therapy.

Arpi M, Vancanneyt M, Swings J, Leisner JJ.

Arhus University Hospital, Skejby, Arhus, Denmark.

Six cases of bacteraemia in hospitalized patients, 5

with a depressed immune status, were caused by

lactobacilli. Sodium dodecyl

sulfate-polyacrylamide gel electrophoresis of

whole-cell proteins and API 50 CH carbohydrate

patterns assigned the causative agents to the species

Lactobacillus rhamnosus, Lactobacillus curvatus,

Lactobacillus delbrueckii subsp. lactis and

Lactobacillus paracasei subsp. paracasei.

Publication Types:

Case Reports

PMID: 12953954 [PubMed - indexed for MEDLINE]  Notario

R, Leardini N, Borda N, Gambande T, Cerutti H. Related

Articles, Links

[Hepatic abscess and bacteremia due to Lactobacillus

rhamnosus]

Rev Argent Microbiol. 2003 Apr-Jun; 35(2): 100-1.

Review. Spanish.

PMID: 12920992 [PubMed - indexed for MEDLINE]

[Guest guest]

I am too sleep deprived to sift through the medical explanation. In lay

man's terms is Culturelle bad for our kids? Or does it depend on the kid? We

ran out of the kydohphilis brand and have been giving Culturelle for the

past week with no negative reactions.

Thanks,

Martha

On Tue, Sep 28, 2010 at 7:42 PM, <thecolemans4@...>wrote:

>

>

> Now for some people, this may not be so bad. Sometimes stimulation is good

> -

> depending on what type of illness. I think it depends on someone's immune

> panel.

> But for PANDAS, OCD, anxiety... whoa. I think TNFa is already associated

> with

> those, and some of the other inflammatory

> cytokines/chemokines/interleukins.

> This is only a small collection of them.

>

>

> Culturelle is manufactured in gelatin capsules

> providing 20 billion colony forming units (CFUs) per

> capsule of Lactobacillus GG.

>

> J Leukoc Biol. 2003 Sep; 74(3): 395-402.

>

> Lactobacilli and streptococci induce inflammatory

> chemokine production in human macrophages that

> stimulates Th1 cell chemotaxis.

>

> Veckman V, Miettinen M, Matikainen S, Lande R,

> Giacomini E, Coccia EM, Julkunen I.

>

> Department of Microbiology, National Public Health

> Institute, Helsinki,

> Finland. ville.veckman@... <ville.veckman%40ktl.fi>

>

> Macrophages have a central role in innate-immune

> responses to bacteria.

> In the present work, we show that infection of human

> macrophages with

> Gram-positive pathogenic Streptococcus pyogenes or

> nonpathogenic Lactobacillus rhamnosus GG enhances mRNA

> expression of inflammatory chemokine ligands

> CCL2/monocyte chemoattractant protein-1 (MCP-1),

> CCL3/macrophage-inflammatory protein-1alpha

> (MIP-1alpha), CCL5/regulated on activation, normal T

> expressed and secreted, CCL7/MCP-3, CCL19/MIP-3beta,

> and CCL20/MIP-3alpha and CXC chemokine igands

> CXCL8/interleukin (IL)-8, CXCL9/monokine induced by

> interferon-gamma (IFN-gamma), and CXCL10/IFN-inducible

> protein 10. Bacteria-induced CCL2, CCL7, CXCL9, and

> CXCL10 mRNA expression was partially dependent on

> ongoing protein synthesis.

>

> The expression of these chemokines and of CCL19 was

> dependent on bacteria-induced IFN-alpha/beta

> production. CCL19 and CCL20 mRNA expression was

> up-regulated by IL-1beta or tumor necrosis factor

> alpha

> (TNF-alpha), and in addition, IFN-alpha together with

> TNF-alpha further enhanced CCL19 gene expression.

> Synergy between IFN-alpha and TNF-alpha was also seen

> for CXCL9 and CXCL10 mRNA expression.

>

> Bacteria-stimulated macrophage supernatants induced

> the migration of T helper cell type 1 (Th1) cells,

> suggesting that in human macrophages, these bacteria

> can stimulate efficient inflammatory chemokine gene

> expression including those that recruit Th1 cells to

> the site of inflammation. Furthermore, L.

> rhamnosus-induced Th1 chemokine production could in

> part explain the proposed antiallergenic properties of

> this bacterium.

>

> PMID: 12949243 [PubMed - indexed for MEDLINE]

>

> J Food Prot. 2003 Mar; 66(3): 466-72. Links

>

> Interactions of lactic acid bacteria with human

> intestinal epithelial cells: effects on cytokine

> production.

>

> Wallace TD, Bradley S, Buckley ND, Green- JM.

>

> Department of Biology, Acadia University, Wolfville,

> Nova Scotia,

> Canada B0P 1X0.

>

> As a participant in the mucosal immune response, the

> intestinal epithelial cell must respond to a variety

> of stimuli, including lactic acid bacteria (LAB)

> consumed in the diet. The objective of this study was

> to compare the abilities of several strains of LAB to

> modulate cytokine secretion by human intestinal

> epithelial cell (IEC) line HT-29. Certain strains of

> Lactobacillus rhamnosus, Lactobacillus delbrueckii,

> and Lactobacillus acidophilus suppressed the

> production of the chemokine RANTES by stimulated HT-29

> IEC, although the magnitude of this suppression varied

> depending on the nature of the bacterial growth

> medium. Similarly, specific strains showed growth

> condition-dependent suppression of HT-29 interleukin-8

> (IL-8) production. Strain-dependent effects were also

> seen for the suppression of tumor necrosis factor

> alpha (TNF-alpha) and transforming growth factor beta

> (TGF-beta) production. The binding of several of these

> bacterial strains to the HT-29 cell line was also

> examined. Different strains were found to have

> differing abilities to interact with IEC, with L.

> rhamnosus R0011 being the strain that generally had

> the most extensive effects on HT-29 cytokine

> production and also bound to HT-29 IEC most

> effectively. Modulation of IEC cytokine production has

> the potential to profoundly affect the mucosal

> microenvironment, influencing the immune response to

> pathogens and other ingested antigens.

>

> PMID: 12636302 [PubMed - indexed for MEDLINE]

>

> : Salminen MK, Rautelin H, Tynkkynen S, Poussa T,

> Saxelin M, Valtonen

> V,

> Jarvinen A.

>

> Lactobacillus bacteremia, clinical significance, and

> patient outcome, with special focus on probiotic L.

> rhamnosus GG.

> Clin Infect Dis. 2004 Jan 1; 38(1): 62-9. Epub 2003

> Dec 04.

> PMID: 14679449 [PubMed - in process]

>

> Scand J Infect Dis. 2003; 35(6-7): 404-8.

>

> Six cases of Lactobacillus bacteraemia: identification

> of organisms and antibiotic susceptibility and

> therapy.

>

> Arpi M, Vancanneyt M, Swings J, Leisner JJ.

> Arhus University Hospital, Skejby, Arhus, Denmark.

>

> Six cases of bacteraemia in hospitalized patients, 5

> with a depressed immune status, were caused by

> lactobacilli. Sodium dodecyl

> sulfate-polyacrylamide gel electrophoresis of

> whole-cell proteins and API 50 CH carbohydrate

> patterns assigned the causative agents to the species

> Lactobacillus rhamnosus, Lactobacillus curvatus,

> Lactobacillus delbrueckii subsp. lactis and

> Lactobacillus paracasei subsp. paracasei.

>

> Publication Types:

> Case Reports

>

> PMID: 12953954 [PubMed - indexed for MEDLINE] Notario

> R, Leardini N, Borda N, Gambande T, Cerutti H. Related

> Articles, Links

> [Hepatic abscess and bacteremia due to Lactobacillus

> rhamnosus]

> Rev Argent Microbiol. 2003 Apr-Jun; 35(2): 100-1.

> Review. Spanish.

> PMID: 12920992 [PubMed - indexed for MEDLINE]

>

>

[Guest guest]

I think I need help understanding this as well. Wouldn't this mean that

MOST probiotics are bad for PANDAS kids since the majority have streptocci

and all that I have seen have some form of lactobacilli? Or is that not the

same as lactobacillus? Not a good thing since these kids are usually on

long term antibiotics.