Re: Dr. G's opinion of Gaba?

[Guest guest]

He took our son off of it.  Its a non medical supplement which he does not

approve of. 

From: Gage Vanlandingham <gager292@...>

Subject: Dr. G's opinion of Gaba?

Date: Monday, March 7, 2011, 11:03 AM

 

Does anyone know if Dr. G or Dr. opposes the use of Gaba to help calm

the kids?

 

Petra

[Guest guest]

Thanks!

From: Gage Vanlandingham <gager292@...>

Subject: Dr. G's opinion of Gaba?

Date: Monday, March 7, 2011, 11:03 AM

 

Does anyone know if Dr. G or Dr. opposes the use of Gaba to help calm

the kids?

 

Petra

[Guest guest]

J Neurochem. 2000 Jun;74(6):2445-54.

GABA(A)-mediated toxicity of hippocampal neurons in vitro.

Lukasiuk K, Pitkänen A.

Epilepsy Research Laboratory, A. I. Virtanen Institute for Molecular Sciences,

University of Kuopio, Finland.

Abstract

In the present study, we examined whether the elevation of GABA by

gamma-vinyl-GABA protects cultured rat fetal hippocampal neurons against

toxicity induced by a 20-min incubation with 100 microM L-glutamate. Neither a

24-h pretreatment nor posttreatment with gamma-vinyl-GABA (100 microM) had any

neuroprotective effects, as determined by counting microtubule-associated

protein-2 positive cells and lactate dehydrogenase assay 24 h after the

glutamate treatment. Unexpectedly, gamma-vinyl-GABA alone induced a 20% loss of

microtubule-associated protein-2-positive cells in a culture that was grown in

medium containing 25 mM KCl. The toxic effect of gamma-vinyl-GABA was mimicked

by a 24-h treatment with GABA (100 microM) and the GABA(A) receptor agonist,

muscimol (10 microM), but not the GABA( receptor agonist, baclofen (10

microM). The GABA(A) receptor antagonist, bicuculline (10 microM), protected

against gamma-vinyl-GABA and GABA-evoked toxicity. Neither gamma-vinyl-GABA nor

GABA was toxic in culture medium containing 15 mM KCl. These data indicate that,

under depolarizing conditions, an increased GABA level is toxic for a

subpopulation of developing hippocampal neurons in vitro. The effect is GABA(A)

receptor-mediated. These data provide a new view for understanding

neurodegenerative processes, and raise a question of the safety of therapies

aimed at increasing GABA concentration following brain insults, especially in

immature brains.

PMID: 10820205 [PubMed - indexed for MEDLINE]

>

> Does anyone know if Dr. G or Dr. opposes the use of Gaba to help calm

the kids?

>  

> Petra

>

>

>

>

>

[Guest guest]

Thanks for the info!

Petra

From: ElyseG <elyse-g@...>

Subject: Re: Dr. G's opinion of Gaba?

Date: Wednesday, March 9, 2011, 2:23 AM

 

J Neurochem. 2000 Jun;74(6):2445-54.

GABA(A)-mediated toxicity of hippocampal neurons in vitro.

Lukasiuk K, Pitkänen A.

Epilepsy Research Laboratory, A. I. Virtanen Institute for Molecular Sciences,

University of Kuopio, Finland.

Abstract

In the present study, we examined whether the elevation of GABA by

gamma-vinyl-GABA protects cultured rat fetal hippocampal neurons against

toxicity induced by a 20-min incubation with 100 microM L-glutamate. Neither a

24-h pretreatment nor posttreatment with gamma-vinyl-GABA (100 microM) had any

neuroprotective effects, as determined by counting microtubule-associated

protein-2 positive cells and lactate dehydrogenase assay 24 h after the

glutamate treatment. Unexpectedly, gamma-vinyl-GABA alone induced a 20% loss of

microtubule-associated protein-2-positive cells in a culture that was grown in

medium containing 25 mM KCl. The toxic effect of gamma-vinyl-GABA was mimicked

by a 24-h treatment with GABA (100 microM) and the GABA(A) receptor agonist,

muscimol (10 microM), but not the GABA( receptor agonist, baclofen (10

microM). The GABA(A) receptor antagonist, bicuculline (10 microM), protected

against gamma-vinyl-GABA and GABA-evoked toxicity. Neither

gamma-vinyl-GABA nor GABA was toxic in culture medium containing 15 mM KCl.

These data indicate that, under depolarizing conditions, an increased GABA level

is toxic for a subpopulation of developing hippocampal neurons in vitro. The

effect is GABA(A) receptor-mediated. These data provide a new view for

understanding neurodegenerative processes, and raise a question of the safety of

therapies aimed at increasing GABA concentration following brain insults,

especially in immature brains.

PMID: 10820205 [PubMed - indexed for MEDLINE]

>

> Does anyone know if Dr. G or Dr. opposes the use of Gaba to help calm

the kids?

>  

> Petra

>

>

>

>

>