Guest guest Posted November 25, 2008 Report Share Posted November 25, 2008 Brain glutathione levels in patients with epilepsy measured by in vivo ^1 H-MRS * S. G. Mueller, MD, A. H. Trabesinger, PhD, P. Boesiger, PhD and H. G. Wieser, MD */Neurology/ 2001;57:1422-1427 http://www.neurology.org/cgi/content/full/57/8/1422 // Objective:/ /--- Glutathione in its reduced form (GSH) is^ the most important free radical scavenging compound in the mammalian^ nervous system that prevents membrane lipid peroxidation. It^ is suspected that epileptic seizures are accompanied by a massive^ production of reactive oxygen species, i.e., oxidative stress.^ //Methods://--- Using an ^1 H MRS technique developed at the authors'^ site, the authors measured glutathione levels in a volume of^ interest (VOI) of 25 x 25 x 25 mm placed in structurally normal-appearing^ tissue in the parietooccipital region of each hemispheres in^ patients with and without active epilepsy, and in a age-matched^ control group.^ //Results://--- The GSH/water ratio in patients with epilepsy^ was significantly reduced in the parietooccipital region of^ both hemispheres (1.6 ± 1.0 x 10^- ^5 ) compared to the GSH/water^ ratio in healthy controls (2.4 ± 1.1 x 10^- ^5 ). There was^ no significant difference between the hemisphere with epileptogenic^ focus and the hemisphere without epileptogenic focus. The GSH/water^ ratios of the patients without active epilepsy were not different^ from the GSH/water ratios of patients with active epilepsy.^ //Conclusion://--- The authors found evidence for a widespread^ impairment of the glutathione system in patients with epilepsy^ independent from seizure activity. http://www.neurology.org/cgi/content/full/57/8/1422 .. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted November 25, 2008 Report Share Posted November 25, 2008 Hi , Once again an amazing article! I am not scientist and was wondering if you could just briefly tell me how they measured the Glutathione. It said water ratio- did they take a specimen from the brain??? I am interested because my son has seizures and this is the only thing he tested low on out of all the DAN protocol tests. I did give him the cream and one IV but saw an INCREASE in seizures so I stopped. I wish this article went further and addressed whether we could replace glutathione effectively. Thank you Thank you thank you for all your posts!!!! -------------- Original message from binstock : -------------- Brain glutathione levels in patients with epilepsy measured by in vivo ^1 H-MRS * S. G. Mueller, MD, A. H. Trabesinger, PhD, P. Boesiger, PhD and H. G. Wieser, MD */Neurology/ 2001;57:1422-1427 http://www.neurology.org/cgi/content/full/57/8/1422 // Objective:/ /--- Glutathione in its reduced form (GSH) is^ the most important free radical scavenging compound in the mammalian^ nervous system that prevents membrane lipid peroxidation. It^ is suspected that epileptic seizures are accompanied by a massive^ production of reactive oxygen species, i.e., oxidative stress.^ //Methods://--- Using an ^1 H MRS technique developed at the authors'^ site, the authors measured glutathione levels in a volume of^ interest (VOI) of 25 x 25 x 25 mm placed in structurally normal-appearing^ tissue in the parietooccipital region of each hemispheres in^ patients with and without active epilepsy, and in a age-matched^ control group.^ //Results://--- The GSH/water ratio in patients with epilepsy^ was significantly reduced in the parietooccipital region of^ both hemispheres (1.6 ± 1.0 x 10^- ^5 ) compared to the GSH/water^ ratio in healthy controls (2.4 ± 1.1 x 10^- ^5 ). There was^ no significant difference between the hemisphere with epileptogenic^ focus and the hemisphere without epileptogenic focus. The GSH/water^ ratios of the patients without active epilepsy were not different^ from the GSH/water ratios of patients with active epilepsy.^ //Conclusion://--- The authors found evidence for a widespread^ impairment of the glutathione system in patients with epilepsy^ independent from seizure activity. http://www.neurology.org/cgi/content/full/57/8/1422 .. Quote Link to comment Share on other sites More sharing options...
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