Once again, antigen-specific IgG antibodies are immunoprote...

[Guest guest]

Forwarded from a colleague. Intriguing at many levels and showing how IgG

may be protective. So, if somebody tests IgG levels, and bases diet on them,

maybe they're avoiding protective foods.

The full PDF is available to those who sign on

_http://www.jacionline.org/article/S0091-6749(12)00440-X/abstract_

(http://www.jacionline.org/article/S0091-6749(12)00440-X/abstract)

Induction and suppression of allergic diarrhea and systemic anaphylaxis in

a murine model of food allergy

Top of Form

· _Zeynep Yesim Kucuk_

(http://www.jacionline.org/article/S0091-6749(12)00440-X/abstract) , MD

_ Strait_

(http://www.ja%20cionline.org/article/S0091-6749(12)00440-X/abstract) , MD

_Marat V.

Khodoun_ (http://www.jacionline.org/article/S0091-6749(12)00440-X/abstract) ,

PhD

_ Mahler_ (http://www.jac

ionline.org/article/S0091-6749(12)00440-X/abstract) , BA _Simon Hogan_

(http://ww%20w.jacionline.org/article/S0091-6749(12)00440-X/abstract) , PhD

_Fred D. Finkelman_

(http://www.jacionline.org/article/S0091-6749(12)00440-X/abstract) , MD

(mailto:ffinkelman@...)

Received 29 October 2011; received in revised form 16 February 2012;

accepted 8 March 2012. published online 02 April 2012.

The Journal of Allergy and Clinical Immunology

_Volume 129, Issue 5_

(http://www.jacionline.org/issues?issue_key=S0091-6749(11)X0020-9) , Pages

1343-1348, May 2012

Background

The clinical manifestations of food allergy include diarrhea and systemic

anaphylaxis (shock), which can occur together or by themselves in different

subjects. Although ingested food antigens need to be absorbed to induce

shock, it is not known whether they need to be absorbed to induce diarrhea.

Objective

We sought to identify mechanisms that determine whether food allergy

induces diarrhea versus shock and determine whether diarrhea requires

absorption

of ingested antigens.

Methods

These issues were studied in mice in active, passive, and hybrid

immunization models. The active model was used to determine the allergic

diarrhea

susceptibility of J chain– and polymeric immunoglobulin receptor–deficient

mice, which are unable to secrete IgA. The hybrid model was used to

determine whether intravenously administered antigen-specific IgG antibody,

which

is not secreted into the gut, can protect against allergic diarrhea, as well

as shock.

Results

Shock, but not diarrhea, was induced in naive mice by using intravenous

IgE anti-trinitrophenyl (TNP) antibody, followed by oral TNP-BSA, whereas

both were induced in mice presensitized with intraperitoneal ovalbumin/alum

plus oral ovalbumin. More TNP-BSA was required to induce shock than diarrhea

in presensitized mice, and intravenous IgG anti-TNP antibody, which is not

secreted into the gut, protected these mice against both diarrhea and

shock. Consistent with this, chicken ovalbumin–immunized J chain– and

polymeric

immunoglobulin receptor–deficient mice, which have high serum IgA levels

but little intestinal IgA, resisted diarrhea induction.

Conclusion

Intestinal immunity and oral antigen dose determine whether diarrhea,

systemic anaphylaxis, or both are induced, and ingested antigen must be

absorbed to induce either response.

Key words: _ IgA_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=IgA & res\

trictName.ymai=ymai) , _IgE_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=IgE & res\

trictName.ymai=ymai)

, _IgG_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=IgG & res\

trictName.ymai=ymai) , _J chain_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=J%20cha\

in & restrictName.ymai=ymai)

, _polymeric immunoglobulin receptor_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=polymer\

ic%20immunoglobulin%20receptor & r

estrictName.ymai=ymai)

Abbreviations used: _OVA_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=OVA & res\

trictName.ymai=ymai) , _Chicken ovalbumin_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=Chick

en%20ovalbumin & restrictName.ymai=ymai) , _pIgR_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=pIgR & re\

strictName.ymai=ymai) ,

_Polymeric immunoglobulin receptor_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=Polymer\

ic%20immunoglobulin%20receptor & rest

rictName.ymai=ymai) , _TNP_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=TNP & res\

trictName.ymai=ymai) , _Trinitrophenyl_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=Trinitr

ophenyl & restrictName.ymai=ymai) , _WT_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=WT & rest\

rictName.ymai=ymai) ,

_Wild-type_

(http://www.jacionline.org/search/quick?search_area=journal & search_text1=Wild-ty\

pe & restrictName.ymai=ymai)

Jan Patenaude, RD, CLT

Director of Medical Nutrition

Oxford Biomedical Technologies, Inc. (formerly Signet Diagnostic Corp.)

Telecommuting Nationwide

(Mountain Time)

Fax:

DineRight4@...

Certified LEAP Therapist (CLT) and specialist in inflammation caused by

non-IgE food sensitivity - which causes IBS, migraine, fibromyalgia,

arthritis and more. Co-author of the Certified LEAP Therapist Training Course

-inexpensive CPE for the RD and learn how to use the Mediator Release Test

(MRT)

and implement the LEAP Diet Protocol.

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