Re: Re: RESEARCH - Cigarette smoke condensate upregulates the gene and protein expression of proinflammatory cytokines in human fibroblast-like synoviocyte line

[Guest guest]

I " think " it means its alright to smoke even if you have RA?

Stan

Not an MD

-------------- Original message --------------

From: " Mimi " <mimi212@...>

Thank you, . I knew if anyone would find the article linking

smoking and RA, you would...........Doreen

>

> J Interferon Cytokine Res. 2008 Aug;28(8):509-21.

>

>

> Cigarette smoke condensate upregulates the gene and protein

> expressionof proinflammatory cytokines in human fibroblast-like

> synoviocyte line.

>

>

> Shizu M, Itoh Y, Sunahara R, Chujo S, Hayashi H, Ide Y, Takii T,

> Koshiko M, Chung SW, Hayakawa K, Miyazawa K, Hirose K, Onozaki K.

> Department of Molecular Health Sciences, Graduate School of

> Pharmaceutical Sciences, Nagoya City University, Mizuho, Nagoya,

> Japan.

>

>

> Rheumatoid arthritis (RA) is characterized by proliferation of

> synoviocytes that produce proinflammatory cytokines, which are

> implicated in the pathogenesis of RA. When human fibroblast-like

> synoviocytes line MH7A was treated with cigarette smoke condensate

> (CSC), either mainstream or sidestream, expression levels of

> interleukin (IL)-1alpha, IL-1beta, IL-6, IL-8, and CYP1A1 mRNA were

> upregulated in both time- and dose-dependent manners. The

> upregulatory effects of CSC on these cytokines were not

> significantly inhibited by alpha-naphthoflavone, an aryl

> hydrocarbon receptor (AhR) antagonist, suggesting that the effects

> of CSC were independent of AhR. Cycloheximide treatment indicated

> that the augmenting effect of CSC on IL-1alpha, IL-1beta and IL-8,

> but not IL-6 and CYP1A1, mRNA expression requires de novo protein

> synthesis. CSC also induced cytokines at protein levels and further

> augmented the effects of tumor necrosis factor alpha on induction

> of these cytokines at both mRNA and protein levels.

>

> These results support the epidemiological studies indicating a

> strong association between heavy cigarette smoking and pathogenesis

> of RA.

>

> PMID: 18729741

>

> http://www.ncbi.nlm.nih.gov/pubmed/18729741

>

>

> Not an MD

>

[Guest guest]

So basically they are saying that smoking makes the Ra worse, if that's the

case I need

To quit smoking mine is bad enough with out something making it worse

-- [ ] Re: RESEARCH - Cigarette smoke condensate upregulates

the gene and protein expression of proinflammatory cytokines in human

fibroblast-like synoviocyte line

Thank you, . I knew if anyone would find the article linking

smoking and RA, you would...........Doreen

>

> J Interferon Cytokine Res. 2008 Aug;28(8):509-21.

>

>

> Cigarette smoke condensate upregulates the gene and protein

> expressionof proinflammatory cytokines in human fibroblast-like

> synoviocyte line.

>

>

> Shizu M, Itoh Y, Sunahara R, Chujo S, Hayashi H, Ide Y, Takii T,

> Koshiko M, Chung SW, Hayakawa K, Miyazawa K, Hirose K, Onozaki K.

> Department of Molecular Health Sciences, Graduate School of

> Pharmaceutical Sciences, Nagoya City University, Mizuho, Nagoya,

> Japan.

>

>

> Rheumatoid arthritis (RA) is characterized by proliferation of

> synoviocytes that produce proinflammatory cytokines, which are

> implicated in the pathogenesis of RA. When human fibroblast-like

> synoviocytes line MH7A was treated with cigarette smoke condensate

> (CSC), either mainstream or sidestream, expression levels of

> interleukin (IL)-1alpha, IL-1beta, IL-6, IL-8, and CYP1A1 mRNA were

> upregulated in both time- and dose-dependent manners. The

> upregulatory effects of CSC on these cytokines were not

> significantly inhibited by alpha-naphthoflavone, an aryl

> hydrocarbon receptor (AhR) antagonist, suggesting that the effects

> of CSC were independent of AhR. Cycloheximide treatment indicated

> that the augmenting effect of CSC on IL-1alpha, IL-1beta and IL-8,

> but not IL-6 and CYP1A1, mRNA expression requires de novo protein

> synthesis. CSC also induced cytokines at protein levels and further

> augmented the effects of tumor necrosis factor alpha on induction

> of these cytokines at both mRNA and protein levels.

>

> These results support the epidemiological studies indicating a

> strong association between heavy cigarette smoking and pathogenesis

> of RA.

>

> PMID: 18729741

>

> http://www.ncbi.nlm.nih.gov/pubmed/18729741

>

>

> Not an MD

>