RESEARCH - New autoantigens in RA: screening 8268 protein arrays with RA patients' sera

[Guest guest]

Ann Rheum Dis. Published Online First: 28 October 2008.

doi:10.1136/ard.2008.096917

Copyright © 2008 BMJ Publishing Group Ltd & European League Against

Rheumatism

-------------------------------------------------------------------------------

Concise Report

New autoantigens in rheumatoid arthritis: screening 8268 protein

arrays with RA patients' sera

Isabelle Auger 1*, Nathalie Balandraud 2, Justyna Rak 1, Nathalie

Lambert 1, Marielle 1 and Roudier 2

1 INSERM UMR 639, France

2 INSERM UMR 639 and APHM, France

Abstract

Objective: To identify new IgG autoantibodies in rheumatoid arthritis

patients' sera.

Methods: We tested serum samples from 19 rheumatoid arthritis patients

with given HLA-DR genotypes, from 7 spondylarthropathy patients, 2

lupus patients, 4 systemic sclerosis patients and 10 healthy

individuals on 8268 human protein arrays.

Results: We identified four antigens (PAD4, PKC beta 1, PIP4K2C and

BRAF) that were recognized almost uniquely by sera from patients with

RA on protein arrays. Using purified proteins, we confirmed that PAD4

and BRAF are recognized almost uniquely by RA patients.

Conclusion: We identified PAD4 and BRAF as RA specific autoantigens.

http://ard.bmj.com/cgi/content/abstract/ard.2008.096917v1?papetoc

Not an MD