RESEARCH - Identification of proteins with increased expression in RA synovial tissues

[Guest guest]

J Rheumatol. 2009 Apr 15.

Identification of Proteins with Increased Expression in Rheumatoid

Arthritis Synovial Tissues.

Chang X, Cui Y, Zong M, Zhao Y, Yan X, Chen Y, Han J.

From the Key Laboratory for Bio-Drugs, Ministry of Health, Research

Center for Medicinal Biotechnology, Shandong Academy of Medical

Sciences, Shandong; and the Orthopedic Surgery Center, Shandong

Qianfoshan Hospital, Shandong, China.

OBJECTIVE: A proteomic approach was applied to discover novel

rheumatoid arthritis (RA)-specific proteins by comparing the

expression profiles of synovial membranes from patients with RA,

osteoarthritis (OA), and ankylosing spondylitis (AS).

METHODS: Synovial tissues were collected from patients with RA (n =

10), OA (n = 10), or AS (n = 6), and healthy controls matched for age

and sex. Proteins were separated by 2-dimensional polyacrylamide gel

electrophoresis, and the proteins with significantly increased

expression in the RA samples were subject to matrix-assisted laser

adsorption-ionization time-of-flight spectrometry. Results were

verified using Western blot and immunohistochemistry. Levels of the

candidate proteins were measured within plasma and synovial fluids

from the RA patients (n = 30), who had disease duration of 3-7 years,

using ELISA. Levels were also measured within plasma from unmedicated

RA patients (n = 41), who had disease duration of 1-6 months.

RESULTS: Compared with the OA and AS tissue samples, the proteins

Ig-kappa light-chain C region, PRDX4, SOD2, TPI, and TXNDC5 were found

with increased expression in synovial tissues of RA patients. PRDX4,

SOD2, TPI, and TXNDC5 had 2-fold or more increase in expression in

some of the early RA plasma samples (58.55%, 31.7%, 26.8%, and 36.6%,

respectively) as compared with the early OA samples and control

samples. TXNDC5 had 2-fold or more increase in expression in 53.3% of

blood samples and 73.3% of synovial fluid samples from patients with

long disease duration of RA as compared with samples from OA and AS

patients.

CONCLUSION: Functional classification indicated that these identified

proteins were related with cell differentiation, glycol metabolism,

immunoactivation, and endogenous antioxidant reaction.

PMID: 19369474

http://www.ncbi.nlm.nih.gov/pubmed/19369474

Not an MD