Guest guest Posted December 11, 2008 Report Share Posted December 11, 2008 Arthritis Research & Therapy 2008, 10:R135doi:10.1186/ar2553 Research article Collagen-specific T-cell repertoire in blood and synovial fluid varies with disease activity in early rheumatoid arthritis Francesco Ria , Romina Penitente* , De Santis* , Chiara Nicolò , e Di Sante , Massimiliano Orsini , Dario Arzani , Fattorossi , Alessandra Battaglia and Gian Franco Ferraccioli Abstract (provisional) Introduction Type II collagen is a DR4/DR1 restricted target of self-reactive T cells sustaining rheumatoid arthritis. The aim of the study was to analyze the T cell receptor repertoire at onset and in different phases of rheumatoid arthritis. Methods We used the CDR3 BV-BJ spectratyping to study the response to human collagen peptide 261-273 in 12 DR4+ rheumatoid arthritis patients (6 at the onset and 6 during the course of the disease) and in 5 healthy DR4+ relatives. Results Results show that the collagen-specific T cell repertoire is quite restricted at the onset of disease, involving approximately 10 rearrangements. Within the studied collagen-specific rearrangements, nearly 75% is shared among patients. While the size of the repertoire used by controls is comparable to that of patients, it is characterized by different T cell receptors. Part of the antigen-specific T cell repertoire is spontaneously enriched in the synovial fluid. The specific T cell repertoire in the periphery was modulated by the therapies and decreased with the remission of the disease. Failure of immunoscope to detect this repertoire was not due to suppression of collagen-driven proliferation in vitro by CD4+ CD25+ T cells. Clinical relapse of the disease was associated to the appearance of the original collagen-specific T cells. Conclusions The collagen-specific T cell receptor repertoire in peripheral blood and synovial fluid is restricted to a limited number of rearrangements in rheumatoid arthritis. The majority of the repertoire is shared between patients with early rheumatoid arthritis and it is modulated by therapy. http://arthritis-research.com/content/10/6/R135/abstract full article: http://arthritis-research.com/content/pdf/ar2553.pdf Not an MD Quote Link to comment Share on other sites More sharing options...
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