RESEARCH - Anti-inflammatory effects of Z23 on LPS-induced inflammatory responses in RAW264.7 macrophages

June 4, 2009

J Ethnopharmacol. 2008 Dec 8;120(3):447-51. Epub 2008 Oct 2.

Anti-inflammatory effects of Z23 on LPS-induced inflammatory responses

in RAW264.7 macrophages.

Hu XD, Yang Y, Zhong XG, Zhang XH, Zhang YN, Zheng ZP, Zhou Y, Tang W,

Yang YF, Hu LH, Zuo JP.

Laboratory of Immunology and Virology, Shanghai University of

Traditional Chinese Medicine, Shanghai Institute of Materia Medica,

Chinese Academy of Sciences, Shanghai 201203, People's Republic of

China.

AIM OF THE STUDY: Fissistigma oldhamii (Hemsl.) Merr, a traditional

Chinese herb medicine, is used for treating rheumatoid arthritis in

China. In our previous study, an effective compound,

7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl) ethyl] propenamide

(Z23), from this herb has showed potent immunosuppressive effects both

in vitro and in vivo. However, its anti-inflammatory effect and

mechanism is still need to explore.

MATERIALS AND METHODS: We examined the in vitro effects of Z23 on the

production of nitric oxide (NO), prostaglandin E2 (PGE2) and cytokines

by lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages.

RESULTS: Z23 significantly decreased the production of PGE2, NO,

tumour necrosis factor alpha (TNFalpha) and IL6 production. Inducible

nitric oxide synthase (iNOS) and cyclooxygenase2 (COX2) gene

expression were also significantly reduced.

CONCLUSIONS: These results demonstrated that Z23 exerted an

anti-inflammatory effect through modulating the synthesis of several

mediators and cytokines involved in the inflammatory process. This

study provided evidence to understand the therapeutic effects of

Fissistigma oldhamii (Hemsl.) Merr and indicated that Z23 has the

potential for treatment of various inflammatory diseases where the

overproduction of NO, PGE2 and inflammatory cytokines has been shown

to play a role, e.g. rheumatoid arthritis.

PMID: 18952160

Not an MD

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