RE: DHT, Vit D and Ibuprofen for PC ?/Dave/Sam

[Guest guest]

I agree with Dave. What’s frustrating is wanting to understand so we can make better choices- so when thinking/reading discussing PCa we have these things plugged in so our natural instinct of what is right for our treatment/choices comes in and rings a bell. Many of us don’t have the focus or the time to breakdown information that is really technical and not for the layperson as Dave says. It just doesn’t become much use and becomes more frustrating. When I’m working with client’s in my field, the first thing I had to learn was the art of communication- being able to discuss things with them so they could understand. My clients were from all backgrounds and I’d be lost if they didn’t do the same for me in explaining/discussing their own knowledge base. I don’t have an answer, but I do think things may be able to be simplified more and/or after for instance saying DHT, there’s the meaning of it, in brackets. Yes, it does take more time, but what’s a little more time when one knows the information is understood. Also, maybe a summary of the writer’s take on what is written- the so- called “in other words’---- something like “ this is what I’ve summarized- don’t take it exactly.. since it’s my interpretation” As I said, no answers here, but a suggestion to put our heads together and find ways to communicate in ways where the information is something that can be used. What adds to the pain we feel, so often we come here to find ways to help save our lives and/or those of our loved ones. No one has the answer and as a great doc said, we walk through a minefield.. I’d say, most of us read eagerly looking for something new, some understanding.. something we can grasp. Thanks for understanding the intention of my email.grace From: ProstateCancerSupport [mailto:ProstateCancerSupport ] On Behalf Of Dave BeckerSent: Wednesday, December 22, 2010 8:06 AMTo: ProstateCancerSupport Subject: Re: DHT, Vit D and Ibuprofen for PC ? This is too technical for me (not a med student or chemist) to understand.What does it say to us laymen with PC? And I'm not sure what DHT is. Dave DHT, Vit D and Ibuprofen for PC ? This is an interesting study, five years old now and not much tofollow except a paper here or there agreeing that DHT can have anapoptotic effect on PC cells.Vit D is cheap, ibuprofen is cheap, DHT is cheap. Not worth running atrial on cheap stuff that cannot be patented. It will take a gutsypatient to get this treatment modality into the clinic.LnCap cells were grown in a control medium containing a). DHT, and .without. Both samples were treated with vitamin D and ibuprofen. " Simultaneous treatment of LNCaP cells grown without DHT with 10 nM1,25(OH)2D3 and 0.2 mM ibuprofen decreased cell growth by 42%(p<0.001, compared to the control cells). This effect was found to beadditive since each single drug reduced cell proliferation by only24%. " However, rather surprisingly, " On the other hand, highly significant synergistic cell growthinhibition (67%, p<0.001) was achieved by combined treatment of1,25(OH)2D3 and ibuprofen in DHT-stimulated LNCaP cells. This combinedtreatment was also found to be effective in decreasing the celltransition from G1- to S-phase (p<0.003) and effective in enhancingapoptosis. " " Ya pays yer money and ya takes yer chances " as Popeye used to say. Ithink he was right.Anticancer Res. 2005 Sep-Oct;25(5):3425-9.The combined treatment of 1,25-dihydroxyvitamin D3 and a non-steroidanti-inflammatory drug is highly effective in suppressing prostatecancer cell line (LNCaP) growth.Gavrilov V, Steiner M, Shany S.Department of Clinical Biochemistry, Faculty of Health Sciences,Ben-Gurion University of the Negev and Soroka Medical Center,Beer-Sheva 84105, Israel.AbstractBACKGROUND: The active metabolite of vitamin D3, 1,25(OH)2D3, is knownto possess anti-proliferative and pro-differentiative activities inprostate cancer (PCa) cells. However, its clinical use is limitedbecause of the risk of hypercalcemia. Concurrent administration oflower doses of 1,25(OH)2D3 together with other anticancer drugs mayhelp to overcome this obstacle and lead to an effective and tolerabletherapy. In the present in vitro study, we investigated the combinedanti-cancer effect of 1,25(OH)2D3 and ibuprofen, a well-knownnon-steroidal anti-inflammatory drug (NSAID) that is also recognizedfor its ability to reduce prostate cancer development.MATERIALS AND METHODS: An androgen-sensitive prostate cancer cell line(LNCaP), grown in medium containing androgen(5alpha-dihydrotestosterone (DHT)) or without it, was treated with1,25(OH)2D3 or ibuprofen alone or with a combination of both drugs.The effects of the treatments on LNCaP cell proliferation, cell cycleand apoptosis were evaluated by the thymidine incorporation method,the propidium iodide method and ELISA, respectively. The unpairedt-test was used for statistical analysis.RESULTS: Simultaneous treatment of LNCaP cells grown without DHT with10 nM 1,25(OH)2D3 and 0.2 mM ibuprofen decreased cell growth by 42%(p<0.001, compared to the control cells). This effect was found to beadditive since each single drug reduced cell proliferation by only24%. On the other hand, highly significant synergistic cell growthinhibition (67%, p<0.001) was achieved by combined treatment of1,25(OH)2D3 and ibuprofen in DHT-stimulated LNCaP cells. This combinedtreatment was also found to be effective in decreasing the celltransition from G1- to S-phase (p<0.003) and effective in enhancingapoptosis.CONCLUSION: Although both 1,25(OH)2D3 and ibuprofen demonstrate invitro anti-carcinogenic activities as a single drug treatment, thepresent results showed that the combined use of 1,25(OH)2D3 withibuprofen is superior to treatment with a single drug.PMID: 16101159 [PubMed - indexed for MEDLINE]--Sam.For your delectation and edification:-http://poetryfromtheprostrateyears.com/