Re: DHT, Vit D and Ibuprofen for PC ?

[Guest guest]

This is too technical for me (not a med student or chemist) to understand.

What does it say to us laymen with PC? And I'm not sure what DHT is.

Dave

DHT, Vit D and Ibuprofen for PC ?

This is an interesting study, five years old now and not much tofollow except a paper here or there agreeing that DHT can have anapoptotic effect on PC cells.Vit D is cheap, ibuprofen is cheap, DHT is cheap. Not worth running atrial on cheap stuff that cannot be patented. It will take a gutsypatient to get this treatment modality into the clinic.LnCap cells were grown in a control medium containing a). DHT, and .without. Both samples were treated with vitamin D and ibuprofen."Simultaneous treatment of LNCaP cells grown without DHT with 10 nM1,25(OH)2D3 and 0.2 mM ibuprofen decreased cell growth by 42%(p<0.001, compared to the control cells). This effect was found to beadditive since each single drug reduced cell proliferation by only24%."However, rather surprisingly,"On the other hand, highly significant synergistic cell growthinhibition (67%, p<0.001) was achieved by combined treatment of1,25(OH)2D3 and ibuprofen in DHT-stimulated LNCaP cells. This combinedtreatment was also found to be effective in decreasing the celltransition from G1- to S-phase (p<0.003) and effective in enhancingapoptosis. ""Ya pays yer money and ya takes yer chances" as Popeye used to say. Ithink he was right.Anticancer Res. 2005 Sep-Oct;25(5):3425-9.The combined treatment of 1,25-dihydroxyvitamin D3 and a non-steroidanti-inflammatory drug is highly effective in suppressing prostatecancer cell line (LNCaP) growth.Gavrilov V, Steiner M, Shany S.Department of Clinical Biochemistry, Faculty of Health Sciences,Ben-Gurion University of the Negev and Soroka Medical Center,Beer-Sheva 84105, Israel.AbstractBACKGROUND: The active metabolite of vitamin D3, 1,25(OH)2D3, is knownto possess anti-proliferative and pro-differentiative activities inprostate cancer (PCa) cells. However, its clinical use is limitedbecause of the risk of hypercalcemia. Concurrent administration oflower doses of 1,25(OH)2D3 together with other anticancer drugs mayhelp to overcome this obstacle and lead to an effective and tolerabletherapy. In the present in vitro study, we investigated the combinedanti-cancer effect of 1,25(OH)2D3 and ibuprofen, a well-knownnon-steroidal anti-inflammatory drug (NSAID) that is also recognizedfor its ability to reduce prostate cancer development.MATERIALS AND METHODS: An androgen-sensitive prostate cancer cell line(LNCaP), grown in medium containing androgen(5alpha-dihydrotestosterone (DHT)) or without it, was treated with1,25(OH)2D3 or ibuprofen alone or with a combination of both drugs.The effects of the treatments on LNCaP cell proliferation, cell cycleand apoptosis were evaluated by the thymidine incorporation method,the propidium iodide method and ELISA, respectively. The unpairedt-test was used for statistical analysis.RESULTS: Simultaneous treatment of LNCaP cells grown without DHT with10 nM 1,25(OH)2D3 and 0.2 mM ibuprofen decreased cell growth by 42%(p<0.001, compared to the control cells). This effect was found to beadditive since each single drug reduced cell proliferation by only24%. On the other hand, highly significant synergistic cell growthinhibition (67%, p<0.001) was achieved by combined treatment of1,25(OH)2D3 and ibuprofen in DHT-stimulated LNCaP cells. This combinedtreatment was also found to be effective in decreasing the celltransition from G1- to S-phase (p<0.003) and effective in enhancingapoptosis.CONCLUSION: Although both 1,25(OH)2D3 and ibuprofen demonstrate invitro anti-carcinogenic activities as a single drug treatment, thepresent results showed that the combined use of 1,25(OH)2D3 withibuprofen is superior to treatment with a single drug.PMID: 16101159 [PubMed - indexed for MEDLINE]--Sam.For your delectation and edification:-http://poetryfromtheprostrateyears.com/

[Guest guest]

>

> Subject: Re: DHT, Vit D and Ibuprofen for PC ?

> To: ProstateCancerSupport

> Date: Wednesday, December 22, 2010, 1:40 PM

> On 12/22/10, Dave Becker inquired:

>

> > This is too technical for me (not a med student or

> chemist) to

> > understand.

> > What does it say to us laymen with PC? And I'm not

> sure what DHT is.

One key phrase to point out with this:

" in vitro models of androgen-sensitive and

androgen-insensitive human prostate cancer cells "

Real world testing in actual human beings rather than in test tubes might have

different results. The vitamin and the pill part are at least mostly harmless.

[Guest guest]

Chuck Maack wrote:

....

> I doubt we will see much follow-up activity in this regard

> until such time as appropriate trials are developed, and I

> certainly wouldn’t trust just any physician to administer

> either high dose testosterone or DHT other than those

> identified in the prior two papers or otherwise known to have

> special expertise.

Hello Chuck,

That makes a lot of sense to me.

Most practicing doctors aren't research scientists. They follow

the advice that the majority of experts recommend. That's what

we all do in our our jobs. But if a doctor is going to go

against conventional wisdom and prescribe treatments that are

thought by many to do harm, he better be a person who is deeply

knowledgeable about what he's doing, not an average Joe who just

wants to try something different.

For myself, I don't understand hormone therapy well enough to

give any advice to anyone about it beyond " maybe you should ask

your doctor about ... "

Hello Sam,

Are you taking DHT treatments? If not, are you considering it?

Do you have a doctor that you trust to work with you on it?

Alan