Re: collagen and arthritis- websites

[Guest guest]

Thank you, Emma, for the articles on collagen.  I'm sure this will answer all

the questions that came up on that subject.  I hadn't known where the

information came from.  You have enlightened me.  Thank You so very much!

 

Yes, it was Dr. Trentham who first put me on A/P and through that protocol, I

made great strides.  I would not be here if it weren't for Minocin.  I urge

all to keep on searching and to follow A/P.  M/P is another variation of A/P.

There are other protocols that use antibiotics as their base.  Some people do

better on one or another variation, but the important thing is to keep those

offending organisms from taking over your body.  It may be the fight of your

life for the rest of your life.  Giving up is not an option.  The best to all

~~~~Dolores & Mike

From: Emma Sand <seneca.whitaker@...>

Subject: rheumatic collagen and arthritis- websites

rheumatic

Date: Thursday, March 19, 2009, 6:19 PM

Hi Everyone,

I saw the inquiries about collagen and arthritis and remembered the studies

that Trentham has done. He is an AP doc in Boston that many of you

know.

http://www.hyperimmuneegg.org/studies/StudyTrentham.htm

   Passive Transfer by Cells of Type II Collagen-Induced Arthritis in Rats

E. Trentham, Roselynn A. Dynesius, and R. Department of

Medicine, Harvard Medical School and B. Brigham Hospital, Boston,

Massachusetts 02115

[image: Small right arrow pointing to:] This article has been cited

by<http://www.pubmedcentral.nih.gov/tocrender.fcgi?artid=371773 & action=cited>oth\

er

articles in PMC.

Abstract

To investigate the role of immunologic hypersensitivity to collagen in the

causation of type II collagen-induced arthritis in rats, passive transfer

experiments were performed. Wistar/ rats used in these experiments were

demonstrated to be histocompatible by prolonged skin graft survival and

mixed lymphocyte cultures. Popliteal lymph node weight assays excluded a

potential for graft-vs.-host reactivity in this strain. 9 of 32 naive rats

developed arthritis after intravenous receipt of pooled spleen and lymph

node cells from donors that had been injected intradermally with type II

collagen emulsified in incomplete Freund's adjuvant. This passively

transferred synovitis was evident clinically as well as histologically. In

control cell transfer experiments involving a total of 97 recipients,

transfer of arthritis was shown to require viable cells sensitized to type

II collagen. These controls included 17 rats receiving cells from

unimmunized donors, 20 recipients of cells from donors injected with

incomplete Freund's adjuvant alone, and 24 recipients of cells from rats

injected with type I collagen in adjuvant. Deliberate addition of

solubilized type II collagen to unsensitized cells at the time of transfer

or injection of heat-killed sensitized cells also did not cause arthritis in

a total of 36 recipients. These latter two control groups indicate that

disease transfer was not the result of antigen carry-over. Intravenous

injection of sera from arthritic donors was incapable of passively

transferring clinical or histologic synovitis in 30 recipients. Thus, these

studies directly implicate immunologic sensitivity to the cartilage type of

collagen in the etiology of this autoimmune disease.

Full text

Full text is available as a scanned copy of the original print version. Get

a printable copy (PDF file) of the *complete

article*<http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=371773 & blobtype=p\

df>(2.2M),

or click on a page image below to browse page by page. Links to

PubMed are also available for *Selected

References*<http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=371773#ref\

erence-sec>

..

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Selected References

These references are in PubMed. This may not be the complete list of

references from this article.

   - Trentham DE, Townes AS, Kang AH. Autoimmunity to type II collagen an

   experimental model of arthritis. J Exp Med. 1977 Sep 1;146(3):857�868. [

   PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0zcCoGC8M3SsD\

_QomvOxCM8PfinKgRgUGcbrOm5xj & reftype=pubmed & artid=371773 & article-id=371773 & iid=1\

1039 & issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%\

7CPubMed%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/8941\

90>

   ]

   - Trentham DE, Townes AS, Kang AH, JR. Humoral and cellular

   sensitivity to collagen in type II collagen-induced arthritis in rats. J

   Clin Invest. 1978 Jan;61(1):89�96.

[PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0DaG0Ao7e5Z5fWh3PM\

2kc-_7Od1bNUMlyktSvtkyX & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & \

issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPub\

Med%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/618915>

   ]

   - DB. Quantitative studies on the mixed lymphocyte interaction in

   rats. I. Conditions and parameters of response. J Exp Med. 1967 Oct 1;126

   (4):625�654.

[PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=08ULkZaomzbXumIfMa\

X1Siu3z1VFDeXy_COLyHANe & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & \

issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPub\

Med%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/6055759>

   ]

   - BILLINGHAM RE, DEFENDIV, SILVERS WK, STEINMULLER D. Quantitative

   studies on the induction of tolerance of skin homografts and on runt

disease

   in neonatal rats. J Natl Cancer Inst. 1962 Feb;28:365�435.

[PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0tIvM6yVfvrThcRNfY\

LCRzikDJCVrL9FnzoUKSkCS & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & \

issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPub\

Med%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/13869199>

   ]

   - Ford WL, Burr W, Simonsen M. A lymph node weight assay for the

   graft-versus-host activity of rat lymphoid cells. Transplantation. 1970

   Sep;10(3):258�266.

[PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0HwRIEOxXf5AAsYdVJ\

tePJdbPkL0lK9loI3bOrkNE & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & \

issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPub\

Med%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/4394274>

   ]

   - GN, Jr, Linsenmayer TF, Newsome DA. Synthesis of type II collagen

   in vitro by embryonic chick neural retina tissue. Proc Natl Acad Sci U S

   A. 1976 Dec;73(12):4420�4423.

[PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0PEpJwmorpQdW13ecB\

zimCqAJ5FTW-EoFJr8KEkzp & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & \

issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPub\

Med%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/1069994>

   ]

   - Linsenmayer TF, GN, Jr, Hay ED. Synthesis of two collagen types

   by embryonic chick corneal epithelium in vitro. Proc Natl Acad Sci U S A.

   1977 Jan;74(1):39�43.

[PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0JQ2r0vBTvoGJnVvQu\

M-76FrEo3j5draR4MEwYQgL & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & \

issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPub\

Med%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/264692>

   ]

   - EJ. Isolation and characterization of a collagen from chick

   cartilage containing three identical alpha chains. Biochemistry. 1971 Apr

   27;10(9):1652�1659.

[PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0hUV21sL3TSLKCSQbL\

LCcP8tph8j6JqkVayG6aQlB & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & \

issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPub\

Med%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/4325605>

   ]

   - Hahn E, Timpl R, EJ. The production of specific antibodies to

   native collagens with the chain compositions, (alpha1(I))3, (alpha1(II))3,

   and (alpha1(I))2alpha 2. J Immunol. 1974 Jul;113(1):421�423.

[PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0xMJ8Mpvam9jMh2Op7\

zpnooWWaTfc3RDyrsBj5QzN & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & \

issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPub\

Med%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/4832314>

   ]

   - Tung KS, Leong C, McCarty T. Pathogenesis of experimental allergic

   orchitis. III. T lymphocyte requirement in local adoptive transfer by

   peritoneal exudate cells. J Immunol. 1977 May;118(5):1774�1779.

[PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0ramIj-Dunf3ldwNPJ\

JCMcwfdbGHpubL-7DlujQQt & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & \

issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPub\

Med%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/300759>

   ]

   - Tung KS, Unanue ER, Dixon FJ. Pathogenesis of experimental allergic

   orchitis. II. The role of antibody. J Immunol. 1971

Jun;106(6):1463�1472.

   [PubMed<http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=04EaHp_Wl0NY\

sRoO3ryA8-3X823_e5CARvCv3pkUl & reftype=pubmed & artid=371773 & article-id=371773 & iid=\

11039 & issue-id=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez\

%7CPubMed%7CRecord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/493\

2774>

   ]

  ------------------------------

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[Guest guest]

Hi Dolores!

You saw Dr. Trentham? What was he like? I'm very curious about him because

I've read all of his studies. He's getting somewhat close to retirement age,

I would think. I've thought about looking him up, when I'm in Boston for

graduate school, and seeing if he would give me any advice or wisdom. He is

the only AP doc I can think of who is in academia, and I hope to make it

there too!

I'm excited for Spring. I should be within six months of having normal labs.

It will be the first time in six years! It's so much fun to rediscover life

again. It's so amazing to feel " normal " again most of the time, and to be

interested again in the same things as everybody else! I'm finally enjoying

things again. Hope you're having a good time too!

Emma

2009/3/19 mike rosner <martysfolks2004@...>

> Thank you, Emma, for the articles on collagen. I'm sure this will

> answer all the questions that came up on that subject. I hadn't known where

> the information came from. You have enlightened me. Thank You so very

> much!

>

> Yes, it was Dr. Trentham who first put me on A/P and through that protocol,

> I made great strides. I would not be here if it weren't for Minocin. I

> urge all to keep on searching and to follow A/P. M/P is another variation

> of A/P. There are other protocols that use antibiotics as their base. Some

> people do better on one or another variation, but the important thing is to

> keep those offending organisms from taking over your body. It may be the

> fight of your life for the rest of your life. Giving up is not an option.

> The best to all ~~~~Dolores & Mike

>

>

>

> From: Emma Sand <seneca.whitaker@... <seneca.whitaker%40gmail.com>>

> Subject: rheumatic collagen and arthritis- websites

> rheumatic <rheumatic%40>

> Date: Thursday, March 19, 2009, 6:19 PM

>

>

> Hi Everyone,

>

> I saw the inquiries about collagen and arthritis and remembered the studies

> that Trentham has done. He is an AP doc in Boston that many of you

> know.

>

> http://www.hyperimmuneegg.org/studies/StudyTrentham.htm

>

> Passive Transfer by Cells of Type II Collagen-Induced Arthritis in Rats

> E. Trentham, Roselynn A. Dynesius, and R. Department of

> Medicine, Harvard Medical School and B. Brigham Hospital, Boston,

> Massachusetts 02115

> [image: Small right arrow pointing to:] This article has been cited

> by<

> http://www.pubmedcentral.nih.gov/tocrender.fcgi?artid=371773 & action=cited

> >other

>

> articles in PMC.

> Abstract

> To investigate the role of immunologic hypersensitivity to collagen in the

> causation of type II collagen-induced arthritis in rats, passive transfer

> experiments were performed. Wistar/ rats used in these experiments

> were

> demonstrated to be histocompatible by prolonged skin graft survival and

> mixed lymphocyte cultures. Popliteal lymph node weight assays excluded a

> potential for graft-vs.-host reactivity in this strain. 9 of 32 naive rats

> developed arthritis after intravenous receipt of pooled spleen and lymph

> node cells from donors that had been injected intradermally with type II

> collagen emulsified in incomplete Freund's adjuvant. This passively

> transferred synovitis was evident clinically as well as histologically. In

> control cell transfer experiments involving a total of 97 recipients,

> transfer of arthritis was shown to require viable cells sensitized to type

> II collagen. These controls included 17 rats receiving cells from

> unimmunized donors, 20 recipients of cells from donors injected with

> incomplete Freund's adjuvant alone, and 24 recipients of cells from rats

> injected with type I collagen in adjuvant. Deliberate addition of

> solubilized type II collagen to unsensitized cells at the time of transfer

> or injection of heat-killed sensitized cells also did not cause arthritis

> in

> a total of 36 recipients. These latter two control groups indicate that

> disease transfer was not the result of antigen carry-over. Intravenous

> injection of sera from arthritic donors was incapable of passively

> transferring clinical or histologic synovitis in 30 recipients. Thus, these

> studies directly implicate immunologic sensitivity to the cartilage type of

> collagen in the etiology of this autoimmune disease.

> Full text

> Full text is available as a scanned copy of the original print version. Get

> a printable copy (PDF file) of the *complete

> article*<

> http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=371773 & blobtype=pdf

> >(2.2M),

> or click on a page image below to browse page by page. Links to

> PubMed are also available for *Selected

> References*<

> http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=371773#reference-sec

> >

> .

> [image: icon of scanned page

> 359]<

> http://www.pubmedcentral.nih.gov/pagerender.fcgi?artid=371773 & pageindex=1>

> 359<

> http://www.pubmedcentral.nih.gov/pagerender.fcgi?artid=371773 & pageindex=1>

> [image: icon of scanned page

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> 360<

> http://www.pubmedcentral.nih.gov/pagerender.fcgi?artid=371773 & pageindex=2>

> [image: icon of scanned page

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> http://www.pubmedcentral.nih.gov/pagerender.fcgi?artid=371773 & pageindex=3>

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>

> Images in this article

> [image: Image]

> Image

> on p.362<

>

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> >

> [image: Image]

> Image

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>

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> >

> Click on the image to see a larger version.

> Selected References

> These references are in PubMed. This may not be the complete list of

> references from this article.

>

> - Trentham DE, Townes AS, Kang AH. Autoimmunity to type II collagen an

> experimental model of arthritis. J Exp Med. 1977 Sep 1;146(3):857�868. [

> PubMed<

>

http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0zcCoGC8M3SsD_QomvOxCM8Pfi\

nKgRgUGcbrOm5xj & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & issue-id\

=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPubMed%7CRe\

cord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/894190

> >

> ]

> - Trentham DE, Townes AS, Kang AH, JR. Humoral and cellular

> sensitivity to collagen in type II collagen-induced arthritis in rats. J

> Clin Invest. 1978 Jan;61(1):89�96.

> [PubMed<

>

http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0DaG0Ao7e5Z5fWh3PM2kc-_7Od\

1bNUMlyktSvtkyX & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & issue-id\

=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPubMed%7CRe\

cord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/618915

> >

> ]

> - DB. Quantitative studies on the mixed lymphocyte interaction in

> rats. I. Conditions and parameters of response. J Exp Med. 1967 Oct

> 1;126

> (4):625�654.

> [PubMed<

>

http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=08ULkZaomzbXumIfMaX1Siu3z1\

VFDeXy_COLyHANe & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & issue-id\

=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPubMed%7CRe\

cord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/6055759

> >

> ]

> - BILLINGHAM RE, DEFENDIV, SILVERS WK, STEINMULLER D. Quantitative

> studies on the induction of tolerance of skin homografts and on runt

> disease

> in neonatal rats. J Natl Cancer Inst. 1962 Feb;28:365�435.

> [PubMed<

>

http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0tIvM6yVfvrThcRNfYLCRzikDJ\

CVrL9FnzoUKSkCS & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & issue-id\

=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPubMed%7CRe\

cord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/13869199

> >

> ]

> - Ford WL, Burr W, Simonsen M. A lymph node weight assay for the

> graft-versus-host activity of rat lymphoid cells. Transplantation. 1970

> Sep;10(3):258�266.

> [PubMed<

>

http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0HwRIEOxXf5AAsYdVJtePJdbPk\

L0lK9loI3bOrkNE & reftype=pubmed & artid=371773 & article-id=371773 & iid=11039 & issue-id\

=11039 & jid=120 & journal-id=120 & FROM=Article%7CCitationRef & TO=Entrez%7CPubMed%7CRe\

cord & rendering-type=normal & & http://www.ncbi.nlm.nih.gov/pubmed/4394274

> >

> ]

> - GN, Jr, Linsenmayer TF, Newsome DA. Synthesis of type II

> collagen

> in vitro by embryonic chick neural retina tissue. Proc Natl Acad Sci U S

> A. 1976 Dec;73(12):4420�4423.

> [PubMed<

>

http://www.pubmedcentral.nih.gov/redirect3.cgi? & & auth=0PEpJwmorpQdW13ecBzimCqAJ5\

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>

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