Bacteria from bile samples- epidemiology and susceptibility of the bacterial strains.

[Guest guest]

Transplant

Proc. 2007 Nov;39(9):2807-11.

Bacteria isolated from bile samples of liver

recipients in the early period after transplantation: epidemiology and

susceptibility of the bacterial strains.

Kawecki D, Chmura A, Pacholczyk M, Lagiewska B, Adadynski L, Wasiak D, Malkowski P, Sawicka-Grzelak A, Rokosz A, Szymanowska A, Swoboda-Kopec E, Wroblewska M, Rowinski W, Durlik M, Paczek L, Luczak M.

Department of General Surgery and Transplantation, Warsaw, Poland.

OBJECTIVE: We estimated the frequency and susceptibility to

antibacterial agents of bacterial isolates from bile samples obtained from 83

liver recipients in the early period after transplantation. PATIENTS

AND METHODS: We prospectively collected data on 83 adult patients undergoing orthotopic liver transplantation (OLT), including bile

samples taken during the first 30 days after OLT from adult liver recipients

suspected to have bile infections. The

isolation/identification of cultured bacteria was performed according to

standard microbiological procedures and commercially available tests. Susceptibility of the strains to antibacterial agents was

determined according to the Clinical and Laboratory Standards Institute (CLSI)

guidelines. RESULTS: Among 210 bile samples obtained

from 79 liver recipients, bacterial cultures were positive in 110 samples from

59 (75%) recipients yielding 156 bacterial strains. The

most commonly isolated species were as follows: gram-positive cocci (109 isolates) with dominance of coagulase-negative

staphylococci (52%) and enterococci (36%); and

gram-negative bacteria, 21 strains from the Enterobacteriaceae

family and 14 of non-fermenting rods. We identified

some multidrug-resistant (MDR) bacterial strains. In the first week after OLT, we investigated samples from

59 patients, yielding 36 bacterial strains. From the

second to the end of the fourth week after OLT, 120 bacterial strains were

isolated from 65 recipients. CONCLUSION: Gram-positive

bacteria comprised 68.5%. The dominance of MDR

gram-positive bacteria may be related to selection by perioperative

antibiotic prophylaxis.PMID: 18021992 [PubMed - in process]

Barb in Texas - Together in the Fight, Whatever it Takes!

Son Ken (33) UC 91 - PSC 99 - Tx 6/21 & 6/30/07 @ Baylor in Dallas