The c.1-260C>T Promoter Variant of CD14 but Not the c.896A>G (p.D299G) Variant of Toll-Like Receptor 4 (TLR4) Genes Is Associated with Inflammatory Bowel Disease

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Vol. 76, No. 3-4, 2007

Original Paper

The c.1-260C>T Promoter Variant of CD14 but Not the c.896A>G (p.D299G) Variant of Toll-Like Receptor 4 (TLR4) Genes Is Associated with Inflammatory Bowel Disease C. Baumgarta, Carsten Büninga, Lars Geerdtsa, Hartmut H. Schmidta, Janine Genschela, Fiedlera, Enno Gentza, Tomas Molnarb, Ferenc Nagyb, Janos Lonovicsb, Herbert Lochsa, Bertram Wiedenmanna, Renate Nickela, Heiko Witta, Axel DignassaaDivision of Gastroenterology and Hepatology, Department of Medicine, Charité Medical School, Humboldt University of Berlin, Berlin, Germany;b1st Department of Medicine, Faculty of Medicine, University of Szeged, Szeged, Hungary

Address of Corresponding Author

Digestion 2007;76:196-202 (DOI: 000112646)

Key Words

Pattern recognition receptor Toll-like receptor Crohn's disease Ulcerative colitis Single-nucleotide variant (polymorphism) Dendritic cells Macrophages Antigen-presenting cells Antigen presentation Innate immunity IBD

Abstract

Background: Inflammatory bowel disease (IBD) results from an aberrant immune response to the indigenous intestinal flora in genetically susceptible hosts. Therefore, the study of candidate genes involved in host pathogen interactions is of key interest.

Methods: In this two-center, retrospective German and Hungarian cohort study, patients with Crohn's disease (CD) (n = 379; German n = 235, Hungarian n = 144) and ulcerative colitis (UC) (n = 263; German n = 145, Hungarian n = 118) and healthy controls (n = 605; German n = 403, Hungarian n = 202) were genotyped for the presence of the CD14 c.1-260C>T promoter variant and the TLR4 c.896A>G (p.D299G) variant by melting curve analysis using fluorescence resonance energy transfer probes. Data were stratified according to the presence of NOD2 (CARD15) mutations and a detailed genotype-phenotype analysis was performed.

Results: In the German cohort the CD14 single-nucleotide polymorphism was associated with UC, but not CD (UC p = 0.016 vs. CD p = 0.190), while the opposite was found in the Hungarian cohort (UC p = 0.083 vs. CD p = 0.019). No association of IBD with the TLR4 single-nucleotide polymorphism was found in either cohort (UC p = 0.430, CD p = 0.783 vs. UC p = 0.745, CD p = 0.383).

Conclusion: IBD appears to be associated with the CD14 c.1-260C>T promoter variant in Germans and Hungarians, but not with the TLR4 c.896A>G (p.D299G) variant.

Copyright © 2007 S. Karger AG, Basel

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