Grape seed extract reduces oxidative stress & fibrosis in experimental biliary obstruction.

June 19, 2007

J Gastroenterol Hepatol. 2007 Jun;22(6):885-92.

Grape seed extract reduces oxidative

stress and fibrosis in experimental biliary obstruction.

Dulundu E, Ozel Y, Topaloglu U, Toklu H, Ercan F, Gedik N, Sener G.

Department of Fifth Surgery, Haydarpasa

Numune Education and Research Hospital, Istanbul, Turkey.

Background and Aim: The aim of this study was to assess the protective effect

of grape seed extract (GSE) against oxidative liver injury and fibrosis induced

by biliary obstruction in rats. Methods: Wistar albino rats were divided into four groups; control

©, GSE-treated, bile duct ligated (BDL), and BDL

and GSE-treated (BDL + GSE) groups. GSE was

administered at a dose of 50 mg/kg a day orally for 28 days. Serum

aspartate aminotransferase

(AST), alanine aminotransferase

(ALT) and lactate dehydrogenase (LDH) levels were determined

to assess liver function and tissue damage, respectively. Tumor

necrosis factor-alpha (TNF-alpha) and antioxidant capacity (AOC) were assayed

in plasma samples. Liver tissues were taken for

determination of the hepatic malondialdehyde (MDA)

and glutathione (GSH) levels, myeloperoxidase (MPO)

activity and collagen content. Production of reactive

oxidants was monitored by chemiluminescence (CL)

assay. Results: Serum AST, ALT, LDH and plasma

TNF-alpha were elevated in the BDL group as compared to the control group and

were significantly decreased with GSE treatment. Plasma

AOC and hepatic GSH level, depressed by BDL, was elevated back to the control

level in the GSE-treated BDL group. Increases in

tissue MDA level, MPO activity and collagen content due to BDL were also

attenuated by GSE treatment. Furthermore, luminol and lucigenin CL values

in the BDL group increased dramatically compared to the control and were

reduced by GSE treatment. Discussion: These results

suggest that GSE protects the liver from oxidative damage following bile duct ligation in rats. This effect

possibly involves the inhibition of neutrophil

infiltration and lipid peroxidation; thus,

restoration of oxidant and antioxidant status in the tissue. PMID: 17565645 [PubMed

- in process]

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