Suppression of Na+/H+ exchanger isoform-3 in human inflammatory bowel disease: Lack of reversal by 5'-aminosalicylate treatment

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Suppression of Na+/H+ exchanger isoform-3 in human inflammatory bowel disease: Lack of reversal by 5'-aminosalicylate treatment

Authors: Iqbal Siddique a; Fuad Hasan a; Islam Khan b

Affiliations:

a Departments of Medicine,

b Biochemistry, Faculty of Medicine, Kuwait University, Kuwait

DOI: 10.1080/00365520802321253

Publication Frequency: 12 issues per year

Published in: Scandinavian Journal of Gastroenterology First Published on: 10 September 2008 Subjects: Gastroenterology; Gastrointestinal & Abdominal Surgery;

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Abstract

Background. Curcumin ameliorates colitis whether it reverses colitis-induced reduction in colonic contractility remains to be investigated.

Objectives. To investigate the effect of curcumin on colitis-induced reduction of carbachol-induced contraction in colon segments from rats treated with trinitrobenzenesulphonic acid.

Methods. Colitis was induced in rats by intra rectal administration of trinitrobenzenesulphonic acid and followed for 5 days. A group of animals which received trinitobenzene sulphonic acids was treated with curcumin (100 mg/Kg and 200 mg/kg body weight) 2 hrs prior to induction of colitis. The controls received phosphate buffered saline in a similar fashion. Markers of inflammation and contractility of colon were assayed using standard procedures.

Results. Induction of colitis was associated with increased myeloperoxidase activity and malondialdehyde levels, gross histological

changes characterized by infiltration of inflammatory cells. All these changes were prevented by treatment with curcumin (100mg/kg). Treatment with curcumin also reduced the histological scores from 3.34±0.40 to 1.75±0.30 confirming an anti-inflammatory effect of curcumin in this experimental model of colitis. Colonic reactivity to carbachol was decreased in colitis affecting the maximum response but not sensitivity. Treatment with curcumin had no effect on sensitivity of the colon to carbachol in any of the preparations. Curcumin however reversed the decrease in carbachol-induced contraction associated with trinitrobenzenesulphonic acid treatment. The same dose of curcumin had no effect on either the potency of or the maximum response to carbachol in control rats. Tissue expression of NF-kB was increased in colon segments from trinitrobenzenesulphonic acid -treated rats and this was inhibited in rats treated with curcumin.

Conclusions. Based on these findings it is concluded that curcumin prevented the reduction in carbachol-induced contraction in trinitrobenzenesulphonic acid -treated rats by modulating NF-kB signaling pathway.

Keywords: Crohn's colitis; IBD; MPO; Na+/H+ exchanger; sodium pump; ulcerative colitis